Plcz1 phospholipase C, zeta 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Plcz1provided by MGI
Official Full Name: phospholipase C, zeta 1provided by MGI
Primary source: MGI:MGI:2150308
See related: Ensembl:ENSMUSG00000030230 AllianceGenome:MGI:2150308
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PLCzeta; 1700041H07Rik
Summary: Enables phosphatidylinositol phospholipase C activity. Involved in positive regulation of cytosolic calcium ion concentration involved in egg activation. Acts upstream of or within calcium ion transport and egg activation. Located in nucleolus; nucleoplasm; and pronucleus. Is expressed in several structures, including alimentary system; heart; integumental system; limb; and nervous system. Human ortholog(s) of this gene implicated in spermatogenic failure 17. Orthologous to human PLCZ1 (phospholipase C zeta 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward testis adult (RPKM 21.0) See more
Orthologs: human all
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Genomic context

Location:: 6 G2; 6 69.77 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (139933118..139987188, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (139987396..140041488, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Plcz1 Deficiency Decreased Fertility in Male Mice Which Is Associated with Sperm Quality Decline and Abnormal Cytoskeleton in Epididymis.
Title: Plcz1 Deficiency Decreased Fertility in Male Mice Which Is Associated with Sperm Quality Decline and Abnormal Cytoskeleton in Epididymis.
Advancing male age differentially alters levels and localization patterns of PLCzeta in sperm and testes from different mouse strains.
Title: Advancing male age differentially alters levels and localization patterns of PLCzeta in sperm and testes from different mouse strains.
Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model.
Title: Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model.
PLCzeta1 is a sperm-borne oocyte activation factor that ensures oocyte activation steps for monospermic fertilization.
Title: Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice.
analysis of the EF-hand domain in targeting sperm phospholipase Czeta to membrane phosphatidylinositol 4,5-bisphosphate
Title: Essential Role of the EF-hand Domain in Targeting Sperm Phospholipase Cζ to Membrane Phosphatidylinositol 4,5-Bisphosphate (PIP2).
Human PLCzeta exhibits superior fertilization potency over mouse PLCzeta in triggering the Ca(2+) oscillations required for mammalian oocyte activation.
Title: Human PLCζ exhibits superior fertilization potency over mouse PLCζ in triggering the Ca(2+) oscillations required for mammalian oocyte activation.
these results suggest that both PLCzeta- and sperm-mediated Ca2+ release preferentially use an intracellular membranous source of PI(4,5)P2, in contrast to PLCdelta1, which targets PI(4,5)P2 in the plasma membrane.
Title: PLCζ causes Ca(2+) oscillations in mouse eggs by targeting intracellular and not plasma membrane PI(4,5)P(2).
Data suggest that positively charged residues within the XY linker play an important role in the PLCzeta interaction with PtdIns(4,5)P(2), a crucial step in generating the Ca(2+) activation signal that is essential for fertilization in mammals.
Title: Phospholipase Cζ binding to PtdIns(4,5)P2 requires the XY-linker region.
Murine PLCZ1 and bovine PLCZ1 possess species-specific differences in activity and suggest potential differences in the mode of action of the protein between the two species.
Title: Species-specific differences in the activity and nuclear localization of murine and bovine phospholipase C zeta 1.
Sequestration of PLCzeta into the PN participates in termination of Ca2+ oscillations at the interphase in the mouse 1-cell embryo.
Title: Relationship between nuclear sequestration of PLCzeta and termination of PLCzeta-induced Ca2+ oscillations in mouse eggs.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (8) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Plcz1 (e-PCR), detects polymorphism
- UniSTS:507157

Plcz1 (e-PCR), detects polymorphism
- UniSTS:546954

34.MMHAP18FLD4.seq (e-PCR)
- UniSTS:124074

34.MMHAP87FRD10.seq (e-PCR)
- UniSTS:124114

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol phospholipase C activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol phospholipase C activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol phospholipase C activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol-3-phosphate binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol-4,5-bisphosphate binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol-5-phosphate binding	ISO Inferred from Sequence Orthology more info
enables phospholipase C activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoric diester hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within calcium ion transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within egg activation	IDA Inferred from Direct Assay more info	PubMed
involved_in egg activation	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cytosolic calcium ion concentration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cytosolic calcium ion concentration involved in egg activation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cytosolic calcium ion concentration involved in egg activation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cytosolic calcium ion concentration involved in egg activation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within single fertilization	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in pronucleus	IDA Inferred from Direct Assay more info	PubMed
located_in sperm head	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Names: PLC-zeta-1; phosphoinositide phospholipase C-zeta-1; phospholipase C zeta

NP_473407.2

EC 3.1.4.11

XP_006506962.1

EC 3.1.4.11

XP_006506963.1

EC 3.1.4.11

XP_006506964.1

EC 3.1.4.11

XP_006506965.1

EC 3.1.4.11

XP_006506967.1

EC 3.1.4.11

XP_017176832.1

EC 3.1.4.11

XP_030110971.1

EC 3.1.4.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_054066.4 → NP_473407.2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

See identical proteins and their annotated locations for NP_473407.2

Status: PROVISIONAL

Source sequence(s)

AC158625

Consensus CDS

CCDS20671.1

UniProtKB/Swiss-Prot

Q3KPE5, Q8K4D7

Related

ENSMUSP00000032356.7, ENSMUST00000032356.13

Conserved Domains (4) summary

cd00275
Location:519 → 644

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

PLN02952
Location:79 → 642

PLN02952; phosphoinositide phospholipase C

pfam09279
Location:80 → 162

EF-hand_like; Phosphoinositide-specific phospholipase C, efhand-like

cl14615
Location:162 → 489

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

139933118..139987188 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006506899.5 → XP_006506962.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1

Conserved Domains (3) summary

PLN02952
Location:79 → 614

PLN02952; phosphoinositide phospholipase C

pfam13499
Location:21 → 73

EF-hand_7; EF-hand domain pair

cl14615
Location:162 → 489

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily
XM_017321343.3 → XP_017176832.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X4

UniProtKB/TrEMBL

Q3KPE4

Conserved Domains (4) summary

cd00275
Location:409 → 527

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd15898
Location:7 → 40

EFh_PI-PLC; EF-hand motif [structural motif]

cl28895
Location:2 → 40

EFh_PI-PLC; EF-hand motif found in eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) isozymes

cl14615
Location:52 → 379

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily
XM_006506902.5 → XP_006506965.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X4

UniProtKB/TrEMBL

Q3KPE4

Conserved Domains (4) summary

cd00275
Location:409 → 527

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd15898
Location:7 → 40

EFh_PI-PLC; EF-hand motif [structural motif]

cl28895
Location:2 → 40

EFh_PI-PLC; EF-hand motif found in eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) isozymes

cl14615
Location:52 → 379

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily
XM_006506904.5 → XP_006506967.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X6

Conserved Domains (2) summary

PLN02952
Location:1 → 347

PLN02952; phosphoinositide phospholipase C

cl14615
Location:1 → 222

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily
XM_006506900.5 → XP_006506963.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2

Conserved Domains (3) summary

PLN02952
Location:79 → 614

PLN02952; phosphoinositide phospholipase C

pfam13499
Location:21 → 73

EF-hand_7; EF-hand domain pair

cl14615
Location:162 → 489

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily
XM_006506901.5 → XP_006506964.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X3

UniProtKB/TrEMBL

Q3KPE4

Conserved Domains (3) summary

PLN02952
Location:79 → 619

PLN02952; phosphoinositide phospholipase C

pfam13499
Location:25 → 73

EF-hand_7; EF-hand domain pair

cl14615
Location:162 → 489

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily
XM_030255111.2 → XP_030110971.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X5

UniProtKB/TrEMBL

Q3KPE4

Conserved Domains (2) summary

PLN02222
Location:7 → 532

PLN02222; phosphoinositide phospholipase C 2

cl14615
Location:52 → 379

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily