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Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 [ Rattus norvegicus (Norway rat) ]

Gene ID: 117276, updated on 11-Apr-2024

Summary

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Official Symbol
Pfkfb3provided by RGD
Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3provided by RGD
Primary source
RGD:619776
See related
Ensembl:ENSRNOG00000018911 AllianceGenome:RGD:619776
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary
Predicted to enable 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Involved in brain development and fructose 2,6-bisphosphate metabolic process. Predicted to be located in nucleoplasm. Predicted to be active in cytosol. Orthologous to human PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Muscle (RPKM 207.9), Adrenal (RPKM 100.3) and 8 other tissues See more
Orthologs
human mouse all
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Genomic context

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Location:
17q12.2
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 17 NC_086035.1 (71893320..71974526)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 17 NC_051352.1 (66983629..67064702)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 17 NC_005116.4 (70632778..70713736)

Chromosome 17 - NC_086035.1Genomic Context describing neighboring genes Neighboring gene interleukin 2 receptor subunit alpha Neighboring gene proteasome 26S subunit, ATPase 6, pseudogene 1 Neighboring gene RNA binding motif protein 17 Neighboring gene U6 spliceosomal RNA Neighboring gene uncharacterized LOC120097756 Neighboring gene uncharacterized LOC134482599 Neighboring gene protein kinase C, theta Neighboring gene uncharacterized LOC134482595

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. [Sleep deprivation aggravates cognitive impairment in septic rats by activating neuronal glycolytic isoenzyme PFKFB3]. Liu Y, et al. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue, 2023 Mar. PMID 36916342
  2. Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy. Zhu Z, et al. Mol Med Rep, 2021 Nov. PMID 34490476, Free PMC Article
  3. Excitotoxic stimulus stabilizes PFKFB3 causing pentose-phosphate pathway to glycolysis switch and neurodegeneration. Rodriguez-Rodriguez P, et al. Cell Death Differ, 2012 Oct. PMID 22421967, Free PMC Article
  4. Characterization of a new liver- and kidney-specific pfkfb3 isozyme that is downregulated by cell proliferation and dedifferentiation. Duran J, et al. Biochem Biophys Res Commun, 2008 Mar 21. PMID 18191036
  5. 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase expression in rat brain during development. Goren N, et al. Brain Res Mol Brain Res, 2000 Jan 10. PMID 10648897

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. [Sleep deprivation aggravates cognitive impairment in septic rats by activating neuronal glycolytic isoenzyme PFKFB3].
    Title: [Sleep deprivation aggravates cognitive impairment in septic rats by activating neuronal glycolytic isoenzyme PFKFB3].
  2. Silencing of PFKFB3 protects podocytes against high glucoseinduced injury by inducing autophagy.
    Title: Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy.
  3. kappa-opioid receptor stimulation alleviates rat vascular smooth muscle cell calcification via PFKFB3-lactate signaling.
    Title: κ-opioid receptor stimulation alleviates rat vascular smooth muscle cell calcification via PFKFB3-lactate signaling.
  4. 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase Suppresses Neuronal Apoptosis by Increasing Glycolysis and ""cyclin-dependent kinase 1-Mediated Phosphorylation of p27 After Traumatic Spinal Cord Injury in Rats.
    Title: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase Suppresses Neuronal Apoptosis by Increasing Glycolysis and "cyclin-dependent kinase 1-Mediated Phosphorylation of p27 After Traumatic Spinal Cord Injury in Rats.
  5. Overexpression of the miR-26a or si-PFKFB3 provides an elevation in cell proliferation. Our study suggests that the miR-26a RNA alleviates lower extremity IRI-induced VEC injury in rats through the activation of the AMPK(AMPK alpha1) pathway by inhibiting PFKFB3.
    Title: Effect of microRNA-26a on vascular endothelial cell injury caused by lower extremity ischemia-reperfusion injury through the AMPK pathway by targeting PFKFB3.
  6. Increased PFKFB3 expression, but not increased PFKFB3 Ser461 phosphorylation, plays a role in increasing glycolysis in mitogen-stimulated thymocytes.
    Title: Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.
  7. astrocyte proliferation can be effectively suppressed by down-regulation of PFKFB3 through control of glycolytic flux, which is downstream of Cdh1
    Title: PFKFB3-mediated glycolysis is involved in reactive astrocyte proliferation after oxygen-glucose deprivation/reperfusion and is regulated by Cdh1.
  8. The PFK2 expression along with LDH-4 were observed to be increased ~2-fold (P < 0.001) in 0.5 mM ammonia treated brain slices.
    Title: Moderate grade hyperammonemia activates lactate dehydrogenase-4 and 6-phosphofructo-2-kinase to support increased lactate turnover in the brain slices.
  9. The expression of PFKFB3 lacking the APC(Cdh1)-targeting KEN motif, promoted accumulation of PFKFB3 and its release from the nucleus to the cytosol through an excess Cdh1-inhibitable process.
    Title: Excitotoxic stimulus stabilizes PFKFB3 causing pentose-phosphate pathway to glycolysis switch and neurodegeneration.
  10. The maximum expression of Pfkfb3 was detected before puberty, while that of Pfkfb4 was observed in adulthood during rat sperm maturation.
    Title: Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.
Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables 6-phosphofructo-2-kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 6-phosphofructo-2-kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables 6-phosphofructo-2-kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables 6-phosphofructo-2-kinase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity TAS
Traceable Author Statement
more info
 PubMed 
Process Evidence Code Pubs
involved_in carbohydrate phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in fructose 2,6-bisphosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 2,6-bisphosphate metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in fructose 2,6-bisphosphate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in fructose metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of glycolytic process TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Names
6PF-2-K/Fru-2,6-P2ASE brain-type isozyme
6PF-2-K/Fru-2,6-P2ase 3
PFK/FBPase 3
RB2K
NP_476476.1
XP_006254254.1
XP_006254255.1
XP_006254258.1
XP_006254259.1
XP_006254260.1
XP_017455933.1
XP_063132102.1
XP_063132103.1
XP_063132104.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_057135.1 → NP_476476.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3

    See identical proteins and their annotated locations for NP_476476.1

    Status: PROVISIONAL

    Source sequence(s)
    D87240
    UniProtKB/Swiss-Prot
    O35096, O35552, O35553, O35554, O35555, O35556, O35557, Q9QWQ5, Q9QWQ6
    UniProtKB/TrEMBL
    A6JLS9, A6JLT1
    Related
    ENSRNOP00000062965.1, ENSRNOT00000068354.4
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 435
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086035.1 Reference GRCr8

    Range
    71893320..71974526
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017600444.3 → XP_017455933.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X9

    UniProtKB/TrEMBL
    A6JLT1

  2. XM_063276032.1 → XP_063132102.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X3

    UniProtKB/TrEMBL
    A0A8I6AUG9

  3. XM_063276034.1 → XP_063132104.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X8

  4. XM_063276033.1 → XP_063132103.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X7

  5. XM_006254193.4 → XP_006254255.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X2

    See identical proteins and their annotated locations for XP_006254255.1

    UniProtKB/TrEMBL
    A6JLS7, A6JLT1
    Related
    ENSRNOP00000025700.4
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 435
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  6. XM_006254192.4 → XP_006254254.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X1

    See identical proteins and their annotated locations for XP_006254254.1

    UniProtKB/TrEMBL
    A6JLS4, A6JLT1
    Related
    ENSRNOP00000040928.4
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 435
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  7. XM_006254198.4 → XP_006254260.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X6

    See identical proteins and their annotated locations for XP_006254260.1

    UniProtKB/TrEMBL
    A6JLS8, A6JLT2
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 435
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  8. XM_006254197.4 → XP_006254259.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X5

    See identical proteins and their annotated locations for XP_006254259.1

    UniProtKB/TrEMBL
    A6JLS5, A6JLS6
    Related
    ENSRNOP00000096463.1
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 435
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  9. XM_006254196.4 → XP_006254258.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X4

    See identical proteins and their annotated locations for XP_006254258.1

    UniProtKB/TrEMBL
    A6JLT0, A6JLT2
    Related
    ENSRNOP00000025731.5
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 435
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O35552.1 GenPept UniProtKB/Swiss-Prot:O35552

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