Ass1 argininosuccinate synthetase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ass1provided by MGI
Official Full Name: argininosuccinate synthetase 1provided by MGI
Primary source: MGI:MGI:88090
See related: Ensembl:ENSMUSG00000076441 AllianceGenome:MGI:88090
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ASS; fold; Ass-1
Summary: Predicted to enable several functions, including argininosuccinate synthase activity; identical protein binding activity; and toxic substance binding activity. Involved in circadian rhythm. Located in mitochondrion. Is expressed in several structures, including alimentary system; central nervous system; integumental system; metanephros; and sensory organ. Used to study citrullinemia. Human ortholog(s) of this gene implicated in citrullinemia; classic citrullinemia; hepatocellular carcinoma; and melanoma. Orthologous to human ASS1 (argininosuccinate synthase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in liver adult (RPKM 1308.6), kidney adult (RPKM 838.7) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 B; 2 21.81 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (31360282..31410682)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (31470270..31520670)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Citrulline depletion by ASS1 is required for proinflammatory macrophage activation and immune responses.
Title: Citrulline depletion by ASS1 is required for proinflammatory macrophage activation and immune responses.
Epithelial argininosuccinate synthetase is dispensable for intestinal regeneration and tumorigenesis.
Title: Epithelial argininosuccinate synthetase is dispensable for intestinal regeneration and tumorigenesis.
findings uncover a new function of p53 in the regulation of Akt signaling and reveal how p53, ASS1, and Akt are interrelated to each other.
Title: Argininosuccinate synthase 1 is an intrinsic Akt repressor transactivated by p53.
ASS1 acetylation by CLOCK exhibits circadian oscillation in human cells and mouse liver, possibly caused by rhythmic interaction between CLOCK and ASS1, leading to the circadian regulation of ASS1 and ureagenesis.
Title: CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.
In diabetic mice, ablation of Ass resulted in diminished endothelium-derived nitric oxide-mediated vascular relaxation responses.
Title: Endothelial arginine resynthesis contributes to the maintenance of vasomotor function in male diabetic mice.
cAMP-induced Ass1 expression is important in controlling the magnitude of decidualization through regulating L-Arg level.
Title: Cyclic adenosine monophosphate-induced argininosuccinate synthase 1 expression is essential during mouse decidualization.
may contribute to liver injury by enhancing nitrosative stress
Title: Ethanol plus the Jo2 Fas agonistic antibody-induced liver injury is attenuated in mice with partial ablation of argininosuccinate synthase.
We demonstrate that the transgenic mouse system reported here has the merit of sensitivity and direct visualization advantage, and is ideal for annotating temporal and spatial expression profiles and the regulation mode of the ASS gene.
Title: A transgenic approach to study argininosuccinate synthetase gene expression.
Thus, extracellular arginine fuels rapid NO production in activated macrophages, and citrulline recycling via Ass1 and Asl is a fail-safe system that sustains optimum NO production.
Title: Sustained generation of nitric oxide and control of mycobacterial infection requires argininosuccinate synthase 1.
Partial argininosuccinate synthase ablation protects only in acute ethanol-induced liver injury by decreasing nitrosative stress but not in a more chronic scenario where oxidative stress and impaired fatty acid beta-oxidation are key events.
Title: Argininosuccinate synthase conditions the response to acute and chronic ethanol-induced liver injury in mice.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (2) 1 citation
Targeted (4) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

SHGC-152309 (e-PCR)
- UniSTS:177470

Ass1 (e-PCR), detects polymorphism
- UniSTS:506742

RH136340 (e-PCR)
- UniSTS:210330

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables amino acid binding	ISO Inferred from Sequence Orthology more info
enables argininosuccinate synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables argininosuccinate synthase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables toxic substance binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within amino acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in arginine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arginine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in argininosuccinate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in argininosuccinate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in aspartate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in cellular response to laminar fluid shear stress	ISO Inferred from Sequence Orthology more info
involved_in circadian rhythm	IDA Inferred from Direct Assay more info	PubMed
involved_in circadian rhythm	ISO Inferred from Sequence Orthology more info
involved_in citrulline metabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of leukocyte cell-cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in response to glucocorticoid	ISO Inferred from Sequence Orthology more info
involved_in response to mycotoxin	ISO Inferred from Sequence Orthology more info
involved_in response to zinc ion	ISO Inferred from Sequence Orthology more info
involved_in urea cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in urea cycle	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell body fiber	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in myelin sheath	HDA more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in perikaryon	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: argininosuccinate synthase

Names: arginosuccinate synthetase 1; citrulline--aspartate ligase

NP_031520.1

EC 6.3.4.5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.