Bub3 BUB3 mitotic checkpoint protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Bub3provided by MGI
Official Full Name: BUB3 mitotic checkpoint proteinprovided by MGI
Primary source: MGI:MGI:1343463
See related: Ensembl:ENSMUSG00000066979 AllianceGenome:MGI:1343463
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: This gene encodes a component of the mitotic spindle assembly complex. The encoded protein is involved in the regulation of chromosome segregation during oocyte meiosis. Disruption of this gene results in the accumulation of mitotic errors and is lethal in the embryonic stage. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 62.1), limb E14.5 (RPKM 56.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F3; 7 74.36 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (131162081..131173625)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (131560367..131571895)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that Zfp207 binds to Bub3 to form a complex and maintains its protein level in oocytes, and that overexpression of Bub3 is able to partially rescue the occurrence of aneuploid eggs in Zfp207-depleted oocytes.
Title: Zfp207 is a Bub3 binding protein regulating meiotic chromosome alignment in mouse oocytes.
as a member of the spindle assembly checkpoint, Bub3 is required for regulation of both meiosis I and II, and is potentially involved in kinetochore-microtubule attachment in mammalian oocytes
Title: Bub3 is a spindle assembly checkpoint protein regulating chromosome segregation during mouse oocyte meiosis.
Cenpa, Cenpb, and Bub3, but not Cenpc, interacted with PARP-1
Title: Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated.
Haploinsufficiency of Bub3 causes a slight increase in chromosome instability but is not clearly associated with a noticeable rise in the probability of tumor formation.
Title: Increased chromosome instability but not cancer predisposition in haploinsufficient Bub3 mice.
subpopulation of dynein, containing the DYNLT3 light chain, may be important for chromosome congression, in addition to having a role in the transport of checkpoint proteins from the kinetochore to the spindle pole
Title: The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, Bub3.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C78067 (e-PCR)
- UniSTS:208215

RH126898 (e-PCR)
- UniSTS:208216

RH136290 (e-PCR)
- UniSTS:210283

PMC316933P1 (e-PCR)
- UniSTS:273062

AU021329 (e-PCR)
- UniSTS:165561

RH126302 (e-PCR)
- UniSTS:163010

AU043350 (e-PCR)
- UniSTS:159704

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in attachment of spindle microtubules to kinetochore	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chromosome segregation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	NAS Non-traceable Author Statement more info	PubMed
involved_in protein localization to kinetochore	ISO Inferred from Sequence Orthology more info
involved_in regulation of chromosome segregation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of bub1-bub3 complex	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	ISO Inferred from Sequence Orthology more info
part_of mitotic checkpoint complex	IBA Inferred from Biological aspect of Ancestor more info
part_of mitotic checkpoint complex	ISO Inferred from Sequence Orthology more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mitotic checkpoint protein BUB3

Names: WD repeat type I transmembrane protein A72.5; budding uninhibited by benzimidazoles 3 homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001317350.2 → NP_001304279.1 mitotic checkpoint protein BUB3 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (a).

Source sequence(s)

AC159994

Consensus CDS

CCDS40160.1

UniProtKB/Swiss-Prot

P97397, Q6ZWM5, Q9WVA3

Conserved Domains (2) summary

sd00039
Location:17 → 54

7WD40; WD40 repeat [structural motif]

cl29593
Location:14 → 296

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_001411927.1 → NP_001398856.1 mitotic checkpoint protein BUB3 isoform b

Status: VALIDATED

Source sequence(s)

AC159994
NM_009774.5 → NP_033904.2 mitotic checkpoint protein BUB3 isoform a

See identical proteins and their annotated locations for NP_033904.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same isoform (a).

Source sequence(s)

AC159994

Consensus CDS

CCDS40160.1

UniProtKB/Swiss-Prot

P97397, Q6ZWM5, Q9WVA3

Related

ENSMUSP00000081547.6, ENSMUST00000084502.7

Conserved Domains (2) summary

sd00039
Location:17 → 54

7WD40; WD40 repeat [structural motif]

cl29593
Location:14 → 296

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

RNA

NR_177983.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC159994

Related

ENSMUST00000207736.2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

131162081..131173625

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006507271.5 → XP_006507334.1 mitotic checkpoint protein BUB3 isoform X1

Conserved Domains (2) summary

sd00039
Location:31 → 65

7WD40; WD40 repeat [structural motif]

cl29593
Location:2 → 186

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...