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43 DNA polymerase [ Escherichia phage T4 ]

Gene ID: 1258685, updated on 18-Aug-2023

Summary

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Gene symbol
43
Gene description
DNA polymerase
Locus tag
T4p047
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia phage T4 (nat-host: Escherichia coli)
Lineage
Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Straboviridae; Tevenvirinae; Tequatrovirus
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Genomic context

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Sequence:
NC_000866.4 (27197..29893, complement)

NC_000866.4Genomic Context describing neighboring genes Neighboring gene immunity to superinfection Neighboring gene iduronate sulfatase Neighboring gene translation repressor Neighboring gene clamp loader of DNA polymerase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000866.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. Li V, et al. J Mol Biol, 2010 Jul 16. PMID 20493878, Free PMC Article
  2. Different behaviors in vivo of mutations in the beta hairpin loop of the DNA polymerases of the closely related phages T4 and RB69. Trzemecka A, et al. J Mol Biol, 2009 Jun 26. PMID 19409904
  3. Interaction between the T4 helicase loading protein (gp59) and the DNA polymerase (gp43): unlocking of the gp59-gp43-DNA complex to initiate assembly of a fully functional replisome. Xi J, et al. Biochemistry, 2005 May 31. PMID 15909989
  4. Structural and biochemical investigation of the role in proofreading of a beta hairpin loop found in the exonuclease domain of a replicative DNA polymerase of the B family. Hogg M, et al. J Biol Chem, 2007 Jan 12. PMID 17098747
  5. Bacteriophage T4 genome. Miller ES, et al. Microbiol Mol Biol Rev, 2003 Mar. PMID 12626685, Free PMC Article

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Amino acid substitutions that confer decreased ability to replicate DNA under low-dGTP conditions or drug sensitivity were identified in the new motif, which suggests that the motif functions in regulating the stability of polymerase complexes.
    Title: Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase.
  2. The authors propose that their beta loops facilitate strand separation, while the residues that form the loop have low structural constraints.
    Title: Different behaviors in vivo of mutations in the beta hairpin loop of the DNA polymerases of the closely related phages T4 and RB69.
  3. Generation of a gp43-gp59-DNA ternary complex is absolutely required for recombination-dependent replication in the bacteriophage T4 system.
    Title: Interaction between the T4 helicase loading protein (gp59) and the DNA polymerase (gp43): unlocking of the gp59-gp43-DNA complex to initiate assembly of a fully functional replisome.
  4. the primer strand is not stably bound within the exonuclease active site in the absence of the beta hairpin loop
    Title: Structural and biochemical investigation of the role in proofreading of a beta hairpin loop found in the exonuclease domain of a replicative DNA polymerase of the B family.
Submit: New GeneRIF Correction

General gene information

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Homology

General protein information

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Preferred Names
DNA polymerase
NP_049662.1
  • possesses DNA synthesis, 3' to 5' exonuclease (ssDNA), dNTP binding, and DNA binding activities; belongs to the DNA polymerase type-B family

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000866.4 Reference assembly

    Range
    27197..29893 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_049662.1 DNA polymerase [Escherichia phage T4]

    See identical proteins and their annotated locations for NP_049662.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P04415
    UniProtKB/TrEMBL
    A0A023ZWF8, A0A097J6Z9, A0A097J7S0, A0A7S9SVB6, D9IE79
    Conserved Domains (1) summary
    PHA02528
    Location:1 → 898
    43; DNA polymerase; Provisional
Nucleotide Protein
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