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T7p22 DNA primase/helicase [ Escherichia phage T7 ]

Gene ID: 1261046, updated on 18-Aug-2023

Summary

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Gene symbol
T7p22
Gene description
DNA primase/helicase
Locus tag
T7p22
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia phage T7 (nat-host: Escherichia coli)
Lineage
Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Autographiviridae; Studiervirinae; Teseptimavirus
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Genomic context

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Sequence:
NC_001604.1 (11565..13265)

NC_001604.1Genomic Context describing neighboring genes Neighboring gene endonuclease Neighboring gene endonuclease Neighboring gene amidase Neighboring gene HNH endonuclease Neighboring gene hypothetical protein Neighboring gene DNA primase/helicase Neighboring gene hypothetical protein Neighboring gene hypothetical protein Neighboring gene hypothetical protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001604.1

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Zinc-binding domain of the bacteriophage T7 DNA primase modulates binding to the DNA template. Lee SJ, et al. J Biol Chem, 2012 Nov 9. PMID 23024359, Free PMC Article
  2. The roles of tryptophans in primer synthesis by the DNA primase of bacteriophage T7. Zhang H, et al. J Biol Chem, 2012 Jul 6. PMID 22605336, Free PMC Article
  3. An in trans interaction at the interface of the helicase and primase domains of the hexameric gene 4 protein of bacteriophage T7 modulates their activities. Zhu B, et al. J Biol Chem, 2009 Aug 28. PMID 19574219, Free PMC Article
  4. DNA recognition by the DNA primase of bacteriophage T7: a structure-function study of the zinc-binding domain. Akabayov B, et al. Biochemistry, 2009 Mar 3. PMID 19206208, Free PMC Article
  5. Acidic residues in the nucleotide-binding site of the bacteriophage T7 DNA primase. Lee SJ, et al. J Biol Chem, 2005 Jul 22. PMID 15917241

See all (20) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. structural model of a bacteriophage T7 DNA helicase-DNA polymerase complex
    Title: An interaction between DNA polymerase and helicase is essential for the high processivity of the bacteriophage T7 replisome.
  2. Zinc-binding domain of the bacteriophage T7 DNA primase modulates binding to the DNA template
    Title: Zinc-binding domain of the bacteriophage T7 DNA primase modulates binding to the DNA template.
  3. Data show that tryptophans Trp-42, -97, and -147 play different but essential roles in T7 DNA primase.
    Title: The roles of tryptophans in primer synthesis by the DNA primase of bacteriophage T7.
  4. The ligation state of the zinc ion was examined by X-ray absorption spectroscopy and biochemical analysis of genetically altered primases, was analysed.
    Title: DNA recognition by the DNA primase of bacteriophage T7: a structure-function study of the zinc-binding domain.
  5. The switch in contacts between Asp(263) and Tyr(411), Lys(408), or Arg(404) in helix E of the adjacent subunit results in conformational changes that modulate helicase and primase activity.
    Title: An in trans interaction at the interface of the helicase and primase domains of the hexameric gene 4 protein of bacteriophage T7 modulates their activities.
  6. Escherichia coli thioredoxin, the processivity factor, binds with bacteriophage T7 DNA polymerase and helicase
    Title: Interactions of Escherichia coli thioredoxin, the processivity factor, with bacteriophage T7 DNA polymerase and helicase.
  7. thioredoxin-binding domain of T7 DNA polymerase is the site of interaction of the phage-encoded helicase/primase (gp4) and ssDNA binding protein (gp2.5)
    Title: A unique loop in T7 DNA polymerase mediates the binding of helicase-primase, DNA binding protein, and processivity factor.
  8. residues Glu-157, Glu-159, Asp-161, Asp-207, Asp-209, and Asp-237 are essential for binding affinity for ATP
    Title: Acidic residues in the nucleotide-binding site of the bacteriophage T7 DNA primase.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

regulatory

  • Loc: 12671 regulatory_class = promoter

General protein information

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Preferred Names
DNA primase/helicase
NP_041975.1
  • gene 4A, primase/helicase [14,15]

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001604.1 Reference assembly

    Range
    11565..13265
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_041975.1 DNA primase/helicase [Escherichia phage T7]

    See identical proteins and their annotated locations for NP_041975.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P03692
    UniProtKB/TrEMBL
    A0A2P0ZKN1, Q6WY43, Q6WYJ7, Q6WYQ2
    Conserved Domains (3) summary
    smart00493
    Location:151 → 225
    TOPRIM; topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins
    cd19483
    Location:308 → 537
    RecA-like_Gp4D_helicase; RecA-like domain of Escherichia coli bacteriophage T7 Gp4D helicase
    cl21601
    Location:12 → 41
    zf-CHC2; CHC2 zinc finger
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P03692.1

Gene LinkOut

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Research Materials