ORF61 ubiquitin E3 ligase ICP0 [Human alphaherpesvirus 3 (Varicella-zoster virus)] - Gene

Summary

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Gene symbol: ORF61
Gene description: ubiquitin E3 ligase ICP0
Locus tag: HHV3_gp62
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Human alphaherpesvirus 3 (strain: Dumas, acronym: HHV-3, acronym: VZV, nat-host: Homo sapiens)
Lineage: Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus
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Genomic context

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Sequence:: NC_001348.1 (103001..104485, complement)

NC_001348.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001348.1

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A spliced latency-associated VZV transcript maps antisense to the ORF61.
Title: A spliced latency-associated VZV transcript maps antisense to the viral transactivator gene 61.
Activation of H2AX and ATM in varicella-zoster virus infected cells is associated with expression of VZV ORF61 and ORF63.
Title: Activation of H2AX and ATM in varicella-zoster virus (VZV)-infected cells is associated with expression of specific VZV genes.
While not needed to inhibit host cell defenses, disruption of the domain comprised of residues 250 to 320 severely impairs VZV growth, transactivation of the immediate early 63 and glycoprotein E genes, and the pathogenesis of VZV skin infection in vivo.
Title: Identification of a hydrophobic domain in varicella-zoster virus ORF61 necessary for ORF61 self-interaction, viral replication, and skin pathogenesis.
Varicella-zoster virus ORF61 is an inhibitor of NF-kappaB during infection of human dendritic cells.
Title: Varicella-zoster virus inhibition of the NF-κB pathway during infection of human dendritic cells: role for open reading frame 61 as a modulator of NF-κB activity.
ORF61 is a SUMO-binding protein and binds to SUMO1 through conserved SUMO-interacting motifs. These motifs are essential for nuclear body disruption in VZV-infected cells but are not required for VZV replication in vitro.
Title: Disruption of PML nuclear bodies is mediated by ORF61 SUMO-interacting motifs and required for varicella-zoster virus pathogenesis in skin.
These results convincingly demonstrate that varicella-zoster virus ORF61 down-modulates the IRF3-mediated IFN-beta pathway by degradation of activated human IRF3 via direct interaction.
Title: Varicella-zoster virus immediate-early protein ORF61 abrogates the IRF3-mediated innate immune response through degradation of activated IRF3.
The authors demonstrate that disruption of the ORF61p RING finger domain by amino acid substitution (Cys19Gly) alters ORF61p intranuclear distribution and abolishes ORF61p-mediated dispersion of Sp100-containing nuclear bodies.
Title: The RING finger domain of Varicella-Zoster virus ORF61p has E3 ubiquitin ligase activity that is essential for efficient autoubiquitination and dispersion of Sp100-containing nuclear bodies.
ORF61p specifically lacks the immune regulatory activities of ICP0, It decreases Sp100 with no effect on PML.
Title: Complementation of a herpes simplex virus ICP0 null mutant by varicella-zoster virus ORF61p.
Sp1 family proteins are important for ORF61 expression and ORF61 contributes to VZV virus replication and pathogenesis.
Title: Regulation of the ORF61 promoter and ORF61 functions in varicella-zoster virus replication and pathogenesis.
data point towards an important role of ORF61 in the fine-regulation of activation of the MAPK pathways and their downstream targets to optimize the availability of cellular factors involved in VZV gene expression
Title: ORF61 protein of Varicella-zoster virus influences JNK/SAPK and p38/MAPK phosphorylation.

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General gene information

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Homology

Orthologs from OrthoDB

regulatory

Loc: c103006-103001 regulatory_class = polyA_signal_sequence

General protein information

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Preferred Names: ubiquitin E3 ligase ICP0

NP_040183.1

VOG classification

Description:

VOGd0141 [T] * Ring finger domain viral protein, (putative) E3 ubiquitin ligase, some members are transcriptional regulators.
RNA Replication, Transcription and Modification

NCBI Reference Sequences (RefSeq)

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Genome Annotation

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Reference assembly

Genomic

NC_001348.1 Reference assembly

Range

103001..104485 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_040183.1 ubiquitin E3 ligase ICP0 [Human alphaherpesvirus 3]

See identical proteins and their annotated locations for NP_040183.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P09309

UniProtKB/TrEMBL

G9IWI5, Q6QCJ5, Q71S71, Q77NN8

Conserved Domains (3) summary

COG5243
Location:15 → 172

HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]

cd00162
Location:19 → 57

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

pfam00097
Location:19 → 57

zf-C3HC4; Zinc finger, C3HC4 type (RING finger)