Limk2 LIM domain kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Limk2provided by MGI
Official Full Name: LIM domain kinase 2provided by MGI
Primary source: MGI:MGI:1197517
See related: Ensembl:ENSMUSG00000020451 AllianceGenome:MGI:1197517
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: whe; Limk-2
Summary: Enables protein kinase activity. Acts upstream of or within several processes, including cornea development in camera-type eye; protein phosphorylation; and spermatogenesis. Located in cis-Golgi network and nucleus. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system gland; and sensory organ. Orthologous to human LIMK2 (LIM domain kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in placenta adult (RPKM 23.2), colon adult (RPKM 19.9) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 A1; 11 2.34 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (3293297..3359238, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (3343297..3409276, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LIMK2 promotes melanoma tumor growth and metastasis through G3BP1-ESM1 pathway-mediated apoptosis inhibition.
Title: LIMK2 promotes melanoma tumor growth and metastasis through G3BP1-ESM1 pathway-mediated apoptosis inhibition.
NGPF2 triggers synaptic scaling up through ALK-LIMK-cofilin-mediated mechanisms.
Title: NGPF2 triggers synaptic scaling up through ALK-LIMK-cofilin-mediated mechanisms.
0.5Gy Xray irradiation induces reorganization of cytoskeleton and differentiation of osteoblasts.
Title: 0.5‑Gy X‑ray irradiation induces reorganization of cytoskeleton and differentiation of osteoblasts.
Metabolites of intestinal microflora upregulate miR-192-5p to suppress proliferation of colon cancer cells via RhoA-ROCK-LIMK2 pathway.
Title: Metabolites of intestinal microflora upregulate miR-192-5p to suppress proliferation of colon cancer cells via RhoA-ROCK-LIMK2 pathway.
LIM kinase 2 (LIMK2) may play an essential role in platelet function.
Title: LIM kinase 2 (LIMK2) may play an essential role in platelet function.
Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function.
Title: Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function.
LIMK1 KO and LIMK2 KO mice were used to investigate the role of LIMK signaling in the cerebral cortical development.
Title: LIMK1 and LIMK2 regulate cortical development through affecting neural progenitor cell proliferation and migration.
LIMK1/2 is an important regulator for cofilin phosphorylation and actin assembly during mouse early embryo development.
Title: Involvement of LIMK1/2 in actin assembly during mouse embryo development.
The small GTPase RhoA regulated LIMK1/2-cofilin to modulate cytoskeletal dynamics during mouse oocyte meiosis.
Title: The small GTPase RhoA regulates the LIMK1/2-cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis.
results demonstrate an important role for LIMKs in central sensitization and the development of chronic pain
Title: Transient inhibition of LIMKs significantly attenuated central sensitization and delayed the development of chronic pain.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (1)
Gene trapped (2)
Targeted (3) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D9Mit34.1 (e-PCR), detects polymorphism
- UniSTS:131903

RH125426 (e-PCR)
- UniSTS:162248

RH127162 (e-PCR)
- UniSTS:208633

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in astral microtubule organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cornea development in camera-type eye	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of vesicle localization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within head development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cilium assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein localization to nucleus	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cis-Golgi network	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: LIM domain kinase 2

Names: LIM kinase 2; LIM motif-containing protein kinase 2

NP_001029202.1

EC 2.7.11.1

NP_001348552.1

EC 2.7.11.1

NP_001389789.1

EC 2.7.11.1

NP_001389790.1

EC 2.7.11.1

NP_001389791.1

EC 2.7.11.1

NP_001389792.1

EC 2.7.11.1

NP_001389793.1

EC 2.7.11.1

NP_034848.1

EC 2.7.11.1

NP_774958.1

EC 2.7.11.1

XP_006514610.1

EC 2.7.11.1

XP_006514615.1

EC 2.7.11.1

XP_030101468.1

EC 2.7.11.1

XP_030101469.1

EC 2.7.11.1

XP_036012255.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001034030.2 → NP_001029202.1 LIM domain kinase 2 isoform c

See identical proteins and their annotated locations for NP_001029202.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks several 5' exons, but has an alternate 5' terminal exon, which results in a downstream translation start codon, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a.

Source sequence(s)

AB012291, AK034900

Consensus CDS

CCDS24360.1

UniProtKB/Swiss-Prot

O54785

UniProtKB/TrEMBL

Q8BS94

Related

ENSMUSP00000105656.3, ENSMUST00000110029.9

Conserved Domains (2) summary

cd14222
Location:150 → 421

STKc_LIMK2; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 2

pfam00595
Location:1 → 49

PDZ; PDZ domain (Also known as DHR or GLGF)
NM_001361623.1 → NP_001348552.1 LIM domain kinase 2 isoform d

Status: VALIDATED

Source sequence(s)

AK034900, AL713875, BY289120, CA316117, CV784400

Consensus CDS

CCDS88122.1

UniProtKB/TrEMBL

Q5NC03

Related

ENSMUSP00000099163.4, ENSMUST00000101640.10

Conserved Domains (4) summary

cd09465
Location:46 → 104

LIM2_LIMK2; The second LIM domain of LIMK2 (LIM domain Kinase 2)

cd14222
Location:316 → 593

STKc_LIMK2; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 2

pfam00595
Location:131 → 215

PDZ; PDZ domain (Also known as DHR or GLGF)

cl02475
Location:15 → 44

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
NM_001402860.1 → NP_001389789.1 LIM domain kinase 2 isoform 3

Status: VALIDATED

Source sequence(s)

AL671968, AL713875
NM_001402861.1 → NP_001389790.1 LIM domain kinase 2 isoform f

Status: VALIDATED

Source sequence(s)

AL671968, AL713875
NM_001402862.1 → NP_001389791.1 LIM domain kinase 2 isoform g

Status: VALIDATED

Source sequence(s)

AL671968, AL713875
NM_001402863.1 → NP_001389792.1 LIM domain kinase 2 isoform h

Status: VALIDATED

Source sequence(s)

AL671968, AL713875
NM_001402864.1 → NP_001389793.1 LIM domain kinase 2 isoform c

Status: VALIDATED

Source sequence(s)

AL671968, AL713875
NM_010718.4 → NP_034848.1 LIM domain kinase 2 isoform a

See identical proteins and their annotated locations for NP_034848.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (a).

Source sequence(s)

AK034900, AK146099, BY253265

Consensus CDS

CCDS24358.1

UniProtKB/Swiss-Prot

O54776, O54785, O55238, Q9QUL4

Related

ENSMUSP00000099162.4, ENSMUST00000101638.4

Conserved Domains (5) summary

cd09465
Location:67 → 125

LIM2_LIMK2; The second LIM domain of LIMK2 (LIM domain Kinase 2)

smart00221
Location:334 → 601

STYKc; Protein kinase; unclassified specificity

cd14222
Location:337 → 608

STKc_LIMK2; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 2

pfam00595
Location:152 → 236

PDZ; PDZ domain (Also known as DHR or GLGF)

cl02475
Location:12 → 65

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
NM_173053.2 → NP_774958.1 LIM domain kinase 2 isoform b

See identical proteins and their annotated locations for NP_774958.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks two 5' exons, but has an alternate 5' terminal exon which includes an alternate translation start codon, compared to variant 1. The encoded isoform (b) has a shorter and distinct N-terminus, compared to isoform a.

Source sequence(s)

AK034900, AK146099, BY289120, CV784400

Consensus CDS

CCDS24359.1

UniProtKB/Swiss-Prot

O54785

UniProtKB/TrEMBL

Q8BS94

Related

ENSMUSP00000099165.4, ENSMUST00000101642.10

Conserved Domains (5) summary

cd09465
Location:46 → 104

LIM2_LIMK2; The second LIM domain of LIMK2 (LIM domain Kinase 2)

smart00221
Location:313 → 580

STYKc; Protein kinase; unclassified specificity

cd14222
Location:316 → 587

STKc_LIMK2; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 2

pfam00595
Location:131 → 215

PDZ; PDZ domain (Also known as DHR or GLGF)

cl02475
Location:15 → 44

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

3293297..3359238 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030245609.2 → XP_030101469.1 LIM domain kinase 2 isoform X6

Related

ENSMUST00000134576.8

Conserved Domains (3) summary

cd14222
Location:259 → 536

STKc_LIMK2; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 2

cl02475
Location:1 → 47

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

pfam00595
Location:74 → 158

PDZ; PDZ domain (Also known as DHR or GLGF)
XM_036156362.1 → XP_036012255.1 LIM domain kinase 2 isoform X5

Conserved Domains (4) summary

cd09465
Location:45 → 103

LIM2_LIMK2; The second LIM domain of LIMK2 (LIM domain Kinase 2)

cd14222
Location:315 → 592

STKc_LIMK2; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 2

pfam00595
Location:130 → 214

PDZ; PDZ domain (Also known as DHR or GLGF)

cl02475
Location:18 → 43

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
XM_006514552.5 → XP_006514615.1 LIM domain kinase 2 isoform X8

Conserved Domains (2) summary

cd14222
Location:150 → 427

STKc_LIMK2; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 2

pfam00595
Location:1 → 49

PDZ; PDZ domain (Also known as DHR or GLGF)
XM_006514547.2 → XP_006514610.1 LIM domain kinase 2 isoform X2

Conserved Domains (4) summary

cd09465
Location:125 → 183

LIM2_LIMK2; The second LIM domain of LIMK2 (LIM domain Kinase 2)

cd14222
Location:395 → 666

STKc_LIMK2; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 2

cl02475
Location:70 → 123

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

pfam00595
Location:210 → 294

PDZ; PDZ domain (Also known as DHR or GLGF)
XM_030245608.1 → XP_030101468.1 LIM domain kinase 2 isoform X1

Conserved Domains (4) summary

cd09465
Location:125 → 183

LIM2_LIMK2; The second LIM domain of LIMK2 (LIM domain Kinase 2)

cd14222
Location:395 → 672

STKc_LIMK2; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 2

cl02475
Location:70 → 123

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

pfam00595
Location:210 → 294

PDZ; PDZ domain (Also known as DHR or GLGF)