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Capn8 calpain 8 [ Mus musculus (house mouse) ]

Gene ID: 170725, updated on 21-Apr-2024

Summary

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Official Symbol
Capn8provided by MGI
Official Full Name
calpain 8provided by MGI
Primary source
MGI:MGI:2181366
See related
Ensembl:ENSMUSG00000038599 AllianceGenome:MGI:2181366
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
nCL-2; nCL-2'
Summary
Enables calcium-dependent cysteine-type endopeptidase activity and identical protein binding activity. Acts upstream of or within calcium-dependent self proteolysis. Predicted to be located in Golgi apparatus. Predicted to be active in cytoplasm. Is expressed in gut; liver; and male reproductive gland or organ. Orthologous to human CAPN8 (calpain 8). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in stomach adult (RPKM 4.6), colon adult (RPKM 1.6) and 6 other tissues See more
Orthologs
human all
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Genomic context

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Location:
1 H5; 1 85.37 cM
Exon count:
22
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (182392314..182462021)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (182564749..182634456)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 46081 Neighboring gene GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus pseudogene Neighboring gene predicted gene, 46248 Neighboring gene coiled-coil domain containing 185 Neighboring gene sushi domain containing 4 Neighboring gene STARR-seq mESC enhancer starr_03290 Neighboring gene STARR-seq mESC enhancer starr_03291

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. A Gastrointestinal Calpain Complex, G-calpain, Is a Heterodimer of CAPN8 and CAPN9 Calpain Isoforms, Which Play Catalytic and Regulatory Roles, Respectively. Hata S, et al. J Biol Chem, 2016 Dec 30. PMID 27881674, Free PMC Article
  2. Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense. Hata S, et al. PLoS Genet, 2010 Jul 29. PMID 20686710, Free PMC Article
  3. Both the conserved and the unique gene structure of stomach-specific calpains reveal processes of calpain gene evolution. Hata S, et al. J Mol Evol, 2001 Sep. PMID 11523006
  4. Mouse population-guided resequencing reveals that variants in CD44 contribute to acetaminophen-induced liver injury in humans. Harrill AH, et al. Genome Res, 2009 Sep. PMID 19416960, Free PMC Article
  5. Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP. Hata S, et al. J Biol Chem, 2006 Apr 21. PMID 16476741

See all (18) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. gastrointestinal-tract-specific calpains 8 and 9 formed a complex we termed "G-calpain," in which both were essential for gastric mucosal defense
    Title: Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense.
  2. nCL-2/calpain 8 is active without calpain regulatory subunit and oligomerizes through C2-like domains
    Title: Stomach-specific calpain, nCL-2/calpain 8, is active without calpain regulatory subunit and oligomerizes through C2-like domains.
  3. nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP
    Title: Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (6)  1 citation
  • Endonuclease-mediated (1) 

General gene information

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Homology

Clone Names

  • MGC159207, MGC159211

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables calcium-dependent cysteine-type endopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables calcium-dependent cysteine-type endopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables calcium-dependent cysteine-type endopeptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables cysteine-type peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within calcium-dependent self proteolysis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proteolysis IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
calpain-8
Names
new calpain 2
stomach-specific M-type calpain
stomach-specific calpain (nCL-2)
NP_001139278.1
NP_570960.2
XP_006496749.1
XP_017174541.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001145806.1 → NP_001139278.1  calpain-8 isoform 2

    See identical proteins and their annotated locations for NP_001139278.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1.
    Source sequence(s)
    AB050203, AK132449
    Consensus CDS
    CCDS48477.1
    UniProtKB/Swiss-Prot
    Q91VA3
    Related
    ENSMUSP00000129549.2, ENSMUST00000168514.7
    Conserved Domains (2) summary
    pfam00648
    Location:46 → 342
    Peptidase_C2; Calpain family cysteine protease
    cl00165
    Location:356 → 381
    Calpain_III; Calpain, subdomain III. Calpains are calcium-activated cytoplasmic cysteine proteinases, participate in cytoskeletal remodeling processes, cell differentiation, apoptosis and signal transduction. Catalytic domain and the two calmodulin-like domains are ...

  2. NM_130890.2 → NP_570960.2  calpain-8 isoform 1

    See identical proteins and their annotated locations for NP_570960.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AB050202, AC123793, AK132449, BC125504
    Consensus CDS
    CCDS35814.1
    UniProtKB/Swiss-Prot
    Q059W1, Q91UZ9, Q91VA3, Q920R7
    UniProtKB/TrEMBL
    A0A0R4J0K6
    Related
    ENSMUSP00000047164.8, ENSMUST00000048941.14
    Conserved Domains (4) summary
    PTZ00183
    Location:611 → 692
    PTZ00183; centrin; Provisional
    pfam00648
    Location:46 → 342
    Peptidase_C2; Calpain family cysteine protease
    pfam01067
    Location:356 → 509
    Calpain_III; Calpain large subunit, domain III
    cl08302
    Location:580 → 635
    EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    182392314..182462021
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017319052.1 → XP_017174541.1  calpain-8 isoform X1

    UniProtKB/TrEMBL
    A0A0A6YVU9
    Conserved Domains (4) summary
    cd00214
    Location:374 → 534
    Calpain_III; Calpain, subdomain III. Calpains are calcium-activated cytoplasmic cysteine proteinases, participate in cytoskeletal remodeling processes, cell differentiation, apoptosis and signal transduction. Catalytic domain and the two calmodulin-like domains are ...
    cd15897
    Location:557 → 584
    EFh_PEF; EF-hand motif [structural motif]
    cl25352
    Location:557 → 659
    EFh_PEF; The penta-EF hand (PEF) family
    pfam00648
    Location:66 → 362
    Peptidase_C2; Calpain family cysteine protease

  2. XM_006496686.3 → XP_006496749.1  calpain-8 isoform X2

    UniProtKB/Swiss-Prot
    Q059W1, Q91UZ9, Q91VA3, Q920R7
    Conserved Domains (4) summary
    cd00214
    Location:374 → 534
    Calpain_III; Calpain, subdomain III. Calpains are calcium-activated cytoplasmic cysteine proteinases, participate in cytoskeletal remodeling processes, cell differentiation, apoptosis and signal transduction. Catalytic domain and the two calmodulin-like domains are ...
    cd15897
    Location:557 → 584
    EFh_PEF; EF-hand motif [structural motif]
    cl25352
    Location:557 → 672
    EFh_PEF; The penta-EF hand (PEF) family
    pfam00648
    Location:66 → 362
    Peptidase_C2; Calpain family cysteine protease

RNA

  1. XR_373596.3 RNA Sequence

  2. XR_004940678.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q91VA3.1 GenPept UniProtKB/Swiss-Prot:Q91VA3

Gene LinkOut

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