Mpeg1 macrophage expressed gene 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mpeg1provided by MGI
Official Full Name: macrophage expressed gene 1provided by MGI
Primary source: MGI:MGI:1333743
See related: Ensembl:ENSMUSG00000046805 AllianceGenome:MGI:1333743
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: P-2; MPS1; PFN2; Mpg-1; mPFN2
Summary: Involved in defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; and pore formation in membrane of other organism. Located in phagocytic vesicle. Is expressed in several structures, including blood vessel; central nervous system; developing vasculature of reproductive system; and retina outer nuclear layer. Human ortholog(s) of this gene implicated in primary immunodeficiency disease. Orthologous to human MPEG1 (macrophage expressed 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in spleen adult (RPKM 17.8), lung adult (RPKM 13.2) and 22 other tissues See more
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Genomic context

Location:: 19 A; 19 8.72 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (12438134..12442649)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (12460770..12465285)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Perforin-2 is a pore-forming effector of endocytic escape in cross-presenting dendritic cells.
Title: Perforin-2 is a pore-forming effector of endocytic escape in cross-presenting dendritic cells.
Mpeg1 is not essential for antibacterial or antiviral immunity, but is implicated in antigen presentation.
Title: Mpeg1 is not essential for antibacterial or antiviral immunity, but is implicated in antigen presentation.
Intracellular Staphylococcus aureus triggers pyroptosis and contributes to inhibition of healing due to perforin-2 suppression.
Title: Intracellular Staphylococcus aureus triggers pyroptosis and contributes to inhibition of healing due to perforin-2 suppression.
Cellular context of IL-33 expression dictates impact on anti-helminth immunity.
Title: Cellular context of IL-33 expression dictates impact on anti-helminth immunity.
An Essential Role for Perforin-2 in Type I IFN Signaling.
Title: An Essential Role for Perforin-2 in Type I IFN Signaling.
our studies indicate that Perforin-2 breaches the envelope of phagocytosed bacteria, facilitating the delivery of proteases and other antimicrobial effectors to sites within the bacterial cell.
Title: Perforin-2 Breaches the Envelope of Phagocytosed Bacteria Allowing Antimicrobial Effectors Access to Intracellular Targets.
These findings suggest that the protective role of Perforin-2(encoded by the macrophage-expressed gene 1, Mpeg1) against listeriosis is based on it limiting the intracellular replication of the pathogen.
Title: Perforin-2 Protects Host Cells and Mice by Restricting the Vacuole to Cytosol Transitioning of a Bacterial Pathogen.
this study shows that primary embryonic and established fibroblasts can be induced by interferons or by intracellular bacterial infection to express a perforin-like mRNA previously described as macrophage-expressed gene 1 (Mpeg1).
Title: Inhibition of intracellular bacterial replication in fibroblasts is dependent on the perforin-like protein (perforin-2) encoded by macrophage-expressed gene 1.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Mpeg1 (e-PCR), detects polymorphism
- UniSTS:470618

NoName (e-PCR)
- UniSTS:224979

PMC27130P3 (e-PCR)
- UniSTS:272377

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables pore-forming activity	IDA Inferred from Direct Assay more info	PubMed
enables wide pore channel activity	IDA Inferred from Direct Assay more info	PubMed
enables wide pore channel activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within adaptive immune response	IEA Inferred from Electronic Annotation more info
NOT involved_in antibacterial innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in antibacterial innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in antigen processing and presentation of exogenous peptide antigen	IDA Inferred from Direct Assay more info	PubMed
involved_in antigen processing and presentation of exogenous peptide antigen via MHC class I	IDA Inferred from Direct Assay more info	PubMed
involved_in defense response to Gram-negative bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to Gram-negative bacterium	ISO Inferred from Sequence Orthology more info
involved_in defense response to Gram-positive bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to Gram-positive bacterium	ISO Inferred from Sequence Orthology more info
involved_in defense response to bacterium	IBA Inferred from Biological aspect of Ancestor more info
involved_in defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in dendritic cell antigen processing and presentation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within innate immune response	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
is_active_in endolysosome	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in phagocytic vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in phagocytic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in phagolysosome membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: macrophage-expressed gene 1 protein

Names: macrophage gene 1 protein; perforin-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001374668.1 → NP_001361597.1 macrophage-expressed gene 1 protein precursor

Status: VALIDATED

Source sequence(s)

AC151730

UniProtKB/TrEMBL

E9QN37

Conserved Domains (1) summary

pfam01823
Location:125 → 341

MACPF; MAC/Perforin domain
NM_010821.2 → NP_034951.2 macrophage-expressed gene 1 protein precursor

Status: VALIDATED

Source sequence(s)

AC151730

UniProtKB/TrEMBL

E9QN37

Related

ENSMUSP00000108573.4, ENSMUST00000081035.9

Conserved Domains (1) summary

pfam01823
Location:125 → 341

MACPF; MAC/Perforin domain