lin-12 lin-12/Notch intracellular domain [Caenorhabditis elegans] - Gene

Summary

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Gene symbol: lin-12
Gene description: lin-12/Notch intracellular domain
Primary source: WormBase:WBGene00003001
Locus tag: CELE_R107.8
See related: AllianceGenome:WB:WBGene00003001
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables RNA polymerase II-specific DNA-binding transcription factor binding activity. Involved in several processes, including cell-cell signaling involved in cell fate commitment; nematode larval development; and oviposition. Located in apical plasma membrane and nucleus. Part of RNA polymerase II transcription regulator complex. Is expressed in several structures, including body wall muscle cell from M lineage; gonadal sheath cell; somatic nervous system; vulva; and vulval precursor cell. Human ortholog(s) of this gene implicated in several diseases, including Adams-Oliver syndrome; Alagille syndrome; CADASIL 1; Hajdu-Cheney syndrome; and autoimmune disease (multiple). Orthologous to several human genes including NOTCH1 (notch receptor 1); NOTCH2 (notch receptor 2); and NOTCH3 (notch receptor 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See lin-12 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 10

Sequence:: Chromosome: III; NC_003281.10 (9060221..9071472, complement)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Reevaluating the relationship between EGL-43 (EVI1) and LIN-12 (Notch) during C. elegans anchor cell invasion.
Title: Reevaluating the relationship between EGL-43 (EVI1) and LIN-12 (Notch) during C. elegans anchor cell invasion.
we investigate orthologs of a transcription complex that acts in mammalian EGFR signaling-lin-1/Elk1, sur-2/Med23, and the Cdk8 Kinase module (CKM).our analysis using cell fate reporters reveals that both EGFR and LIN-12/Notch signal transduction pathways are active in all VPCs in lin-1/Elk1 mutants
Title: Integration of EGFR and LIN-12/Notch Signaling by LIN-1/Elk1, the Cdk8 Kinase Module, and SUR-2/Med23 in Vulval Precursor Cell Fate Patterning in Caenorhabditis elegans.
Molecular and genetic analyses indicate that the loss of ubr-5 function suppresses defects caused by reduced signaling via GLP-1 or LIN-12.
Title: UBR-5, a Conserved HECT-Type E3 Ubiquitin Ligase, Negatively Regulates Notch-Type Signaling in Caenorhabditis elegans.
LIN-12/Notch signaling mediates type-2 vulval muscle cell development of muscle arms by regulating UNC-40 and MADD-2 activity.
Title: LIN-12/Notch signaling instructs postsynaptic muscle arm development by regulating UNC-40/DCC and MADD-2 in Caenorhabditis elegans.
These findings provide additional evidence highlighting the roles of LIN-12/Notch signaling in coordinating the formation of various components of the functional C. elegans egg-laying system.
Title: A role of the LIN-12/Notch signaling pathway in diversifying the non-striated egg-laying muscles in C. elegans.
LIN-12 signaling is necessary for the suppression of lag-1 expression in the anchor cell in Caenorhabditis elegans.
Title: LIN-12/Notch regulates lag-1 and lin-12 expression during anchor cell/ventral uterine precursor cell fate specification.
Here we identify two cis-regulatory regions from lin-12, both of them are specified by the presence of a conserved EXD/HOX composite binding site. the two Notch paralogs in the C. elegans nervous system.
Title: Identification of novel cis-regulatory regions from the Notch receptor genes lin-12 and glp-1 of Caenorhabditis elegans.
The termination of NOTCH signaling is tightly linked to cell-cycle progression.
Title: Cell-cycle regulation of NOTCH signaling during C. elegans vulval development.
LIN-14 inhibition of LIN-12 contributes to precision and timing of C. elegans vulval fate patterning.
Title: LIN-14 inhibition of LIN-12 contributes to precision and timing of C. elegans vulval fate patterning.
Data show that cdc-42 has the genetic properties consistent with negative regulation of lin-12 activity during vulval precursor cell fate specification.
Title: sel-11 and cdc-42, two negative modulators of LIN-12/Notch activity in C. elegans.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

R107.8 (e-PCR)
- UniSTS:307869

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables Notch binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transmembrane signaling receptor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in Notch signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in Notch signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Notch signaling pathway	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in cell fate specification	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell projection morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dauer exit	IGI Inferred from Genetic Interaction more info	PubMed
involved_in egg-laying behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in nematode larval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of mesodermal cell fate specification	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of basement membrane organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell fate specification	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mesodermal cell fate specification	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of mesodermal cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of vulval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of vulval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sleep	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vulval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vulval development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RNA polymerase II transcription regulator complex	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: lin-12/Notch intracellular domain

NP_499007.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

9060221..9071472 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_066606.7 → NP_499007.1 lin-12/Notch intracellular domain [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_499007.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P14585

Conserved Domains (10) summary

smart00004
Location:635 → 668

NL; Domain found in Notch and Lin-12

cd00054
Location:365 → 401

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00204
Location:1160 → 1292

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam00066
Location:722 → 750

Notch; LNR domain

pfam02711
Location:1306 → 1373

Pap_E4; E4 protein

pfam06816
Location:754 → 804

NOD; NOTCH protein

pfam07684
Location:842 → 898

NODP; NOTCH protein

pfam12796
Location:1167 → 1271

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:1094 → 1147

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:1162 → 1201

ANK; ANK repeat [structural motif]