Padi4 peptidyl arginine deiminase, type IV [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Padi4provided by MGI
Official Full Name: peptidyl arginine deiminase, type IVprovided by MGI
Primary source: MGI:MGI:1338898
See related: Ensembl:ENSMUSG00000025330 AllianceGenome:MGI:1338898
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pad4; Pdi4
Summary: Enables protein-arginine deiminase activity. Involved in histone citrullination; nucleosome assembly; and stem cell population maintenance. Predicted to be part of protein-containing complex. Predicted to be active in cytoplasm and nucleus. Is expressed in blastocyst; early embryo; and oocyte. Orthologous to human PADI4 (peptidyl arginine deiminase 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in thymus adult (RPKM 6.8), bladder adult (RPKM 5.0) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 D3; 4 72.34 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (140472819..140501514, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (140745508..140774239, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Transcriptomic analysis of PADI4 target genes during multi-lineage differentiation of embryonic stem cells.
Title: Transcriptomic analysis of PADI4 target genes during multi-lineage differentiation of embryonic stem cells.
An African-Specific Variant of TP53 Reveals PADI4 as a Regulator of p53-Mediated Tumor Suppression.
Title: An African-Specific Variant of TP53 Reveals PADI4 as a Regulator of p53-Mediated Tumor Suppression.
The Prominent Role of Hematopoietic Peptidyl Arginine Deiminase 4 in Arthritis: Collagen- and Granulocyte Colony-Stimulating Factor-Induced Arthritis Model in C57BL/6 Mice.
Title: The Prominent Role of Hematopoietic Peptidyl Arginine Deiminase 4 in Arthritis: Collagen- and Granulocyte Colony-Stimulating Factor-Induced Arthritis Model in C57BL/6 Mice.
Factors Associated with Promoted Proliferation of Osteosarcoma by Peptidylarginine Deiminase 4.
Title: Factors Associated with Promoted Proliferation of Osteosarcoma by Peptidylarginine Deiminase 4.
Peptidylarginine Deiminase 2 Knockout Improves Survival in hemorrhagic shock.
Title: Peptidylarginine Deiminase 2 Knockout Improves Survival in hemorrhagic shock.
TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4.
Title: TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4.
Peptidylarginine Deiminase 4 Promotes the Renal Infiltration of Neutrophils and Exacerbates the TLR7 Agonist-Induced Lupus Mice.
Title: Peptidylarginine Deiminase 4 Promotes the Renal Infiltration of Neutrophils and Exacerbates the TLR7 Agonist-Induced Lupus Mice.
PAD4 exacerbates ischemic acute kidney injury and inflammation by promoting renal tubular NF-kappaB activity and inflammation via NEMO citrullination.
Title: Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO.
Data suggest that mixed lineage kinase domain-like (MLKL) activation leads to loss of intracellular architecture and chromatin decondensation, but peptidylarginine deiminase 4 (PAD4) is only required for the externalization of neutrophil extracellular trap (NET).
Title: The pseudokinase MLKL activates PAD4-dependent NET formation in necroptotic neutrophils.
Our data support the notion that NETs promote atherosclerosis and that the use of specific PAD4 inhibitors may have therapeutic benefits in this potentially devastating condition.
Title: Myeloid-Specific Deletion of Peptidylarginine Deiminase 4 Mitigates Atherosclerosis.

Submit: New GeneRIF Correction See all GeneRIFs (28)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (5) 1 citation
Endonuclease-mediated (6) 1 citation

General gene information

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Markers

AB013850 (e-PCR)
- UniSTS:163353

NoName (e-PCR)
- UniSTS:235081

Padi4 (e-PCR), detects polymorphism
- UniSTS:546915

Padi6 (e-PCR), detects polymorphism
- UniSTS:531316

D4Mit64 (e-PCR), detects polymorphism
- UniSTS:130181

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables histone H3R17 arginine deiminase activity	ISO Inferred from Sequence Orthology more info
enables histone H3R2 arginine deiminase activity	ISO Inferred from Sequence Orthology more info
enables histone H3R26 arginine deiminase activity	ISO Inferred from Sequence Orthology more info
enables histone H3R8 arginine deiminase activity	ISO Inferred from Sequence Orthology more info
enables histone arginine deiminase activity	IBA Inferred from Biological aspect of Ancestor more info
enables histone arginine deiminase activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein-arginine deiminase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-arginine deiminase activity	ISO Inferred from Sequence Orthology more info
enables protein-arginine deiminase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within innate immune response	IEA Inferred from Electronic Annotation more info
involved_in nucleosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-translational protein modification	ISO Inferred from Sequence Orthology more info
involved_in stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein-arginine deiminase type-4

Names: PAD type IV; peptidylarginine deiminase IV; protein-arginine deiminase type IV

NP_035191.2

EC 3.5.3.15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011061.2 → NP_035191.2 protein-arginine deiminase type-4

See identical proteins and their annotated locations for NP_035191.2

Status: VALIDATED

Source sequence(s)

AB013850, AI645912, AK137625, BY544067

Consensus CDS

CCDS18854.1

UniProtKB/Swiss-Prot

A2AMU3, Q75WC7, Q9Z183

Related

ENSMUSP00000026381.7, ENSMUST00000026381.7

Conserved Domains (3) summary

pfam03068
Location:286 → 663

PAD; Protein-arginine deiminase (PAD)

pfam08526
Location:1 → 111

PAD_N; Protein-arginine deiminase (PAD) N-terminal domain

pfam08527
Location:113 → 272

PAD_M; Protein-arginine deiminase (PAD) middle domain