Por cytochrome p450 oxidoreductase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Porprovided by MGI
Official Full Name: cytochrome p450 oxidoreductaseprovided by MGI
Primary source: MGI:MGI:97744
See related: Ensembl:ENSMUSG00000005514 AllianceGenome:MGI:97744
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CPR; CYPOR; P450R; 4933424M13Rik
Summary: Enables oxidoreductase activity. Predicted to be involved in several processes, including cellular organofluorine metabolic process; negative regulation of hydrolase activity; and positive regulation of steroid biosynthetic process. Predicted to be located in membrane and mitochondrion. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; limb; metanephros; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in congenital adrenal hyperplasia and cytochrome P450 oxidoreductase deficiency. Orthologous to human POR (cytochrome p450 oxidoreductase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in adrenal adult (RPKM 159.7), lung adult (RPKM 143.3) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 G2; 5 75.34 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (135698894..135764180)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (135670040..135735326)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Membrane Damage during Ferroptosis Is Caused by Oxidation of Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1.
Title: Membrane Damage during Ferroptosis Is Caused by Oxidation of Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1.
Hepatic cytochrome b5/P450 reductase contribute to the bioactivation of ellipticine in vitro, but not in vivo.
Title: Application of hepatic cytochrome b(5)/P450 reductase null (HBRN) mice to study the role of cytochrome b(5) in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine.
Knockout of the cytochrome P450 reductase by CRISPR/Cas9 technology (POR(-/-)) in HEK293 cells overexpressing Nox4 or Nox5 did not interfere with ROS production in intact cells. However, POR(-/-) abolished the signal in NADPH-stimulated assays using membrane fractions from the very same cells. Moreover, membranes of rat smooth muscle cells treated with angiotensin II showed an increased NADPH-dependent signal with lucigen
Title: Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays.
we observed that miR-378 modulates the oscillation amplitudes of Cdkn1a in the control of cell cycle and Por in the regulation of oxidation reduction by forming partnership with different circadian transcription factors
Title: Oscillating primary transcripts harbor miRNAs with circadian functions.
Results are the first to show that some forms of opioid-mediated stress-induced analgesia require brain neuronal P450 activity.
Title: Significance of neuronal cytochrome P450 activity in opioid-mediated stress-induced analgesia.
Immunological and metabolic changes resulting from a genetic deficiency in CPR expression in the intestine in mice.
Title: Mice deficient in intestinal epithelium cytochrome P450 reductase are prone to acute toxin-induced mucosal damage.
An apparent link exists between the cytochrome p450 reductase and FGF signaling pathways.
Title: Conditional deletion of cytochrome p450 reductase in osteoprogenitor cells affects long bone and skull development in mice recapitulating antley-bixler syndrome: role of a redox enzyme in development.
These data collectively indicate a novel role of the Cpr gene in fear conditioning and memory.
Title: Neurobehavioral abnormalities in a brain-specific NADPH-cytochrome P450 reductase knockout mouse model.
this study provides novel insights into the regulation of gap junction function by CYPOR and suggests that Cx43 may play an important role(s) in CYPOR-mediated bone defects seen in patients.
Title: Regulation of gap junction function and Connexin 43 expression by cytochrome P450 oxidoreductase (CYPOR).
Data suggest that cellular cytochrome P450 oxidoreductase-dependent cholesterol synthesis is essential during limb and skeletal development.
Title: Cholesterol metabolism: the main pathway acting downstream of cytochrome P450 oxidoreductase in skeletal development of the limb.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (7) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Por (e-PCR), detects polymorphism
- UniSTS:507167

D5Mit60 (e-PCR), detects polymorphism
- UniSTS:130598

D17571 (e-PCR)
- UniSTS:163373

POR (e-PCR)
- UniSTS:253997

PMC180925P1 (e-PCR)
- UniSTS:271671

Por (e-PCR), detects polymorphism
- UniSTS:256629

stSG598014 (e-PCR)
- UniSTS:442757

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables FMN binding	IBA Inferred from Biological aspect of Ancestor more info
enables FMN binding	ISO Inferred from Sequence Orthology more info
enables NADP binding	ISO Inferred from Sequence Orthology more info
enables NADPH-hemoprotein reductase activity	IBA Inferred from Biological aspect of Ancestor more info
enables NADPH-hemoprotein reductase activity	ISO Inferred from Sequence Orthology more info
enables cytochrome-b5 reductase activity, acting on NAD(P)H	ISO Inferred from Sequence Orthology more info
enables electron transfer activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin adenine dinucleotide binding	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	ISO Inferred from Sequence Orthology more info
enables iron-cytochrome-c reductase activity	ISO Inferred from Sequence Orthology more info
enables nitric oxide dioxygenase NAD(P)H activity	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular organofluorine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in demethylation	ISO Inferred from Sequence Orthology more info
involved_in electron transport chain	ISO Inferred from Sequence Orthology more info
involved_in fatty acid oxidation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in nitrate catabolic process	ISO Inferred from Sequence Orthology more info
involved_in nitric oxide catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cholesterol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chondrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of monooxygenase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smoothened signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of steroid hormone biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of cholesterol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of growth plate cartilage chondrocyte proliferation	ISO Inferred from Sequence Orthology more info
involved_in response to dexamethasone	ISO Inferred from Sequence Orthology more info
involved_in response to hormone	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: NADPH--cytochrome P450 reductase

Names: NADH cytochrome P450 oxydoreductase; P450 (cytochrome) oxidoreductase

NP_032924.1

EC 1.6.2.4

XP_006504461.1

EC 1.6.2.4

XP_006504462.1

EC 1.6.2.4

XP_036020784.1

EC 1.6.2.4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001428562.1 → NP_001415491.1 NADPH--cytochrome P450 reductase isoform 1

Status: VALIDATED

Source sequence(s)

AC083948

UniProtKB/Swiss-Prot

P37040
NM_001428563.1 → NP_001415492.1 NADPH--cytochrome P450 reductase isoform 1

Status: VALIDATED

Source sequence(s)

AC083948

UniProtKB/Swiss-Prot

P37040
NM_001428564.1 → NP_001415493.1 NADPH--cytochrome P450 reductase isoform 1

Status: VALIDATED

Source sequence(s)

AC083948

UniProtKB/Swiss-Prot

P37040
NM_001428565.1 → NP_001415494.1 NADPH--cytochrome P450 reductase isoform 1

Status: VALIDATED

Source sequence(s)

AC083948

UniProtKB/Swiss-Prot

P37040
NM_001428566.1 → NP_001415495.1 NADPH--cytochrome P450 reductase isoform 2

Status: VALIDATED

Source sequence(s)

AC083948
NM_008898.3 → NP_032924.1 NADPH--cytochrome P450 reductase isoform 1

See identical proteins and their annotated locations for NP_032924.1

Status: VALIDATED

Source sequence(s)

AC083948

Consensus CDS

CCDS39318.1

UniProtKB/Swiss-Prot

P37040

UniProtKB/TrEMBL

Q05DV1

Related

ENSMUSP00000005651.7, ENSMUST00000005651.13

Conserved Domains (2) summary

cd06204
Location:277 → 677

CYPOR; NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via ...

pfam00258
Location:82 → 219

Flavodoxin_1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

135698894..135764180

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006504399.1 → XP_006504462.1 NADPH--cytochrome P450 reductase isoform X2

Conserved Domains (2) summary

cd06204
Location:277 → 677

CYPOR; NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via ...

pfam00258
Location:82 → 219

Flavodoxin_1
XM_006504398.1 → XP_006504461.1 NADPH--cytochrome P450 reductase isoform X2

Conserved Domains (2) summary

cd06204
Location:277 → 677

CYPOR; NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via ...

pfam00258
Location:82 → 219

Flavodoxin_1
XM_036164891.1 → XP_036020784.1 NADPH--cytochrome P450 reductase isoform X1

Conserved Domains (2) summary

cd06204
Location:328 → 728

CYPOR; NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via ...

pfam00258
Location:133 → 270

Flavodoxin_1