Txk TXK tyrosine kinase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Txkprovided by MGI
Official Full Name: TXK tyrosine kinaseprovided by MGI
Primary source: MGI:MGI:102960
See related: Ensembl:ENSMUSG00000054892 AllianceGenome:MGI:102960
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rlk; Btkl; PTK4; PTK-RL-18; A130089B16Rik
Summary: Predicted to enable non-membrane spanning protein tyrosine kinase activity. Involved in several processes, including T cell receptor signaling pathway; activation of phospholipase C activity; and positive regulation of cytokine production. Acts upstream of or within NK T cell differentiation. Predicted to be located in cytoplasm and nucleus. Is expressed in several structures, including hemolymphoid system; lung; male reproductive gland or organ; stomach mesentery; and vertebral axis musculature. Orthologous to human TXK (TXK tyrosine kinase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in thymus adult (RPKM 5.4), spleen adult (RPKM 1.6) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 C3.2; 5 38.44 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (72853321..72910337, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (72695978..72752992, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Effects of Txkmediated activation of NFkappaB signaling pathway on neurological deficit and oxidative stress after ischemiareperfusion in rats.
Title: Effects of Txk‑mediated activation of NF‑κB signaling pathway on neurological deficit and oxidative stress after ischemia‑reperfusion in rats.
Taken together, these data indicate that ITK plus RLK inhibition may have therapeutic potential in Th1-mediated inflammatory diseases.
Title: A Small Molecule Inhibitor of ITK and RLK Impairs Th1 Differentiation and Prevents Colitis Disease Progression.
TXK plays an overlapping role with ITK in iNKT cell development and function. ITK also has a unique function in the survival of iNKT cells, likely via a p53-dependent pathway.
Title: A unique role for ITK in survival of invariant NKT cells associated with the p53-dependent pathway in mice.
Tec kinases Rlk and Itk together provide important signals for terminal maturation, efficient cytokine production, and peripheral survival of natural killer T (NKT) cells.
Title: The Tec kinases Itk and Rlk regulate NKT cell maturation, cytokine production, and survival.
A role for Rlk kinase is demonstrated in CD8+ T cell signaling and function both in vitro and in vivo.
Title: Tec kinases Itk and Rlk are required for CD8+ T cell responses to virus infection independent of their role in CD4+ T cell help.
Tec kinases play key roles in regulating TCR-mediated polarization of integrins and signaling molecules to the site of TCR stimulation as well as the up-regulation of integrin adhesion.
Title: Tec kinases regulate TCR-mediated recruitment of signaling molecules and integrin-dependent cell adhesion.
The protein data combined with the association constants for binding of each proline-rich peptide to the corresponding SH3 domain provide an explanation for the opposing modes of self-association within the otherwise closely related Rlk and Itk proteins
Title: Determinants of intra versus intermolecular self-association within the regulatory domains of Rlk and Itk.
Tec kinases differentially regulate development of conventional versus nonconventional lymphocytes
Title: Altered development of CD8+ T cell lineages in mice deficient for the Tec kinases Itk and Rlk.
indicate a critical role for Itk and Rlk in conventional CD8(+) T cell development in the thymus
Title: The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Txk (e-PCR), detects polymorphism
- UniSTS:166297

D43963 (e-PCR)
- UniSTS:124834

Txk (e-PCR), detects polymorphism
- UniSTS:256991

D5Nds2 (e-PCR), detects polymorphism
- UniSTS:142561

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables non-membrane spanning protein tyrosine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within NK T cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in T cell receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in T cell receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in activation of phospholipase C activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in adaptive immune response	IBA Inferred from Biological aspect of Ancestor more info
involved_in adaptive immune response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within interleukin-4 production	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cytokine production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of type II interferon production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of type II interferon-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of gene expression	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within type II interferon production	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: tyrosine-protein kinase TXK

Names: resting lymphocyte kinase

NP_001116226.1

EC 2.7.10.2

NP_001276423.1

EC 2.7.10.2

NP_001276424.1

EC 2.7.10.2

NP_038726.2

EC 2.7.10.2

XP_011239024.1

EC 2.7.10.2

XP_036020891.1

EC 2.7.10.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001122754.2 → NP_001116226.1 tyrosine-protein kinase TXK isoform 1

See identical proteins and their annotated locations for NP_001116226.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform 1.

Source sequence(s)

AC099698, AC122733, AK138786, U16145

Consensus CDS

CCDS51514.1

UniProtKB/Swiss-Prot

P42682

UniProtKB/TrEMBL

Q0VBP6

Related

ENSMUSP00000109234.4, ENSMUST00000113604.10

Conserved Domains (4) summary

cd10398
Location:143 → 248

SH2_Tec_Txk; Src homology 2 (SH2) domain found in Tec protein, Txk

cd11907
Location:85 → 139

SH3_TXK; Src Homology 3 domain of TXK, also called Resting lymphocyte kinase (Rlk)

pfam07714
Location:271 → 520

Pkinase_Tyr; Protein tyrosine kinase

cl21453
Location:266 → 525

PKc_like; Protein Kinases, catalytic domain
NM_001289494.1 → NP_001276423.1 tyrosine-protein kinase TXK isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.

Source sequence(s)

AC099698, AC122733

Consensus CDS

CCDS80299.1

UniProtKB/TrEMBL

A0A0G2JG94, Q0VBP6

Related

ENSMUSP00000143476.2, ENSMUST00000197313.5

Conserved Domains (4) summary

pfam07714
Location:249 → 498

Pkinase_Tyr; Protein tyrosine kinase

cl15255
Location:126 → 226

SH2; Src homology 2 (SH2) domain

cl17036
Location:85 → 127

SH3; Src Homology 3 domain superfamily

cl21453
Location:244 → 503

PKc_like; Protein Kinases, catalytic domain
NM_001289495.1 → NP_001276424.1 tyrosine-protein kinase TXK isoform 4

See identical proteins and their annotated locations for NP_001276424.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses two alternate splice sites in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus than isoform 1.

Source sequence(s)

AC099698, AC122733, AK138786

Consensus CDS

CCDS80298.1

UniProtKB/TrEMBL

Q0VBP6

Related

ENSMUSP00000143002.2, ENSMUST00000198464.3

Conserved Domains (3) summary

cd10398
Location:89 → 194

SH2_Tec_Txk; Src homology 2 (SH2) domain found in Tec protein, Txk

cd11907
Location:31 → 85

SH3_TXK; Src Homology 3 domain of TXK, also called Resting lymphocyte kinase (Rlk)

cl21453
Location:212 → 471

PKc_like; Protein Kinases, catalytic domain
NM_013698.3 → NP_038726.2 tyrosine-protein kinase TXK isoform 2

See identical proteins and their annotated locations for NP_038726.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus but is the same size as isoform 1.

Source sequence(s)

AC122733, AK138786, BC137710, D43963

Consensus CDS

CCDS19334.1

UniProtKB/TrEMBL

B2RQ20, Q0VBP6

Related

ENSMUSP00000129397.2, ENSMUST00000169534.6

Conserved Domains (4) summary

cd10398
Location:143 → 248

SH2_Tec_Txk; Src homology 2 (SH2) domain found in Tec protein, Txk

cd11907
Location:85 → 139

SH3_TXK; Src Homology 3 domain of TXK, also called Resting lymphocyte kinase (Rlk)

pfam07714
Location:271 → 520

Pkinase_Tyr; Protein tyrosine kinase

cl21453
Location:266 → 525

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

72853321..72910337 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011240722.1 → XP_011239024.1 tyrosine-protein kinase TXK isoform X2

See identical proteins and their annotated locations for XP_011239024.1

UniProtKB/TrEMBL

Q0VBP6

Conserved Domains (3) summary

cd10398
Location:89 → 194

SH2_Tec_Txk; Src homology 2 (SH2) domain found in Tec protein, Txk

cd11907
Location:31 → 85

SH3_TXK; Src Homology 3 domain of TXK, also called Resting lymphocyte kinase (Rlk)

cl21453
Location:212 → 471

PKc_like; Protein Kinases, catalytic domain
XM_036164998.1 → XP_036020891.1 tyrosine-protein kinase TXK isoform X1

UniProtKB/TrEMBL

Q0VBP6

Conserved Domains (3) summary

cd10398
Location:172 → 277

SH2_Tec_Txk; Src homology 2 (SH2) domain found in Tec protein, Txk

cd11907
Location:114 → 168

SH3_TXK; Src Homology 3 domain of TXK, also called Resting lymphocyte kinase (Rlk)

cl21453
Location:295 → 543

PKc_like; Protein Kinases, catalytic domain