Nox3 NADPH oxidase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nox3provided by MGI
Official Full Name: NADPH oxidase 3provided by MGI
Primary source: MGI:MGI:2681162
See related: Ensembl:ENSMUSG00000023802 AllianceGenome:MGI:2681162
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: het; GP91-3; nmf250
Summary: This gene encodes a member of the NOX family of NADPH oxidases. These enzymes catalyze the transfer of electrons from NADPH to molecular oxygen to produce superoxide and other reactive oxygen species (ROS). The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia, which are crystalline structures of the inner ear involved in the perception of gravity and linear acceleration. In mouse mutations of this gene lead to the absence of otoconia and vestibular dysfunction. [provided by RefSeq, Jun 2013]
Expression: Restricted expression toward testis adult (RPKM 1.0) See more
Orthologs: human all
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Genomic context

Location:: 17 A1; 17 2.05 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (3685515..3746536, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (3635240..3696261, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Functional cooperation between two otoconial proteins Oc90 and Nox3.
Title: Functional cooperation between two otoconial proteins Oc90 and Nox3.
NOX3 mutation impairs SHH signaling and increases cerebellar neural stem cell proliferation.
Title: Mutation in NADPH oxidase 3 (NOX3) impairs SHH signaling and increases cerebellar neural stem/progenitor cell proliferation.
Hsp70 activates Stat3 and suppresses Nox3 via TLR4
Title: An Endothelial Hsp70-TLR4 Axis Limits Nox3 Expression and Protects Against Oxidant Injury in Lungs.
Together, these findings reveal that Nox2 is a Zn(2+)-regulated enzyme that mediates ZnD-induced oxidative stress and kidney hypertrophy. Understanding the specific mechanisms by which ZnD contributes to kidney damage may have an important impact on the treatment of chronic kidney disease.
Title: NADPH oxidase-2 mediates zinc deficiency-induced oxidative stress and kidney damage.
Nox3 mutants and heterozygotes demonstrated a greater susceptibility to noise-induced hearing loss.
Title: Genome-wide association study identifies nox3 as a critical gene for susceptibility to noise-induced hearing loss.
Although Nox3 overexpression revealed no apparent effect, depletion of Nox3 decreased the number of spermatogonial stem cells in both cultured spermatogonia and freshly isolated testis cells.
Title: ROS-Generating Oxidase Nox3 Regulates the Self-Renewal of Mouse Spermatogonial Stem Cells.
Mutations of Nox3 include retrovirus insertion, missense mutations, splice donor mutation, a splice acceptor mutation and premature translational termination.
Title: Molecular characterization of an allelic series of mutations in the mouse Nox3 gene.
palmitate-induced ROS generation and that NOX3-derived ROS may drive palmitate-induced hepatic insulin resistance through JNK and p38(MAPK) pathways.
Title: The effects of palmitate on hepatic insulin resistance are mediated by NADPH Oxidase 3-derived reactive oxygen species through JNK and p38MAPK pathways.
NOX3 is a relevant source of ROS generation in the cochlear and vestibular systems and that NOX3-dependent ROS generation might contribute to hearing loss and balance problems in response to ototoxic drugs.
Title: NOX3, a superoxide-generating NADPH oxidase of the inner ear.
an allelic series of mutations at the otoconia-deficient head tilt (het) locus, affecting the gene for NADPH oxidase 3 (Nox3), identifies Nox3 as a protein with a clear enzymatic function indispensable for otoconia morphogenesis
Title: Vestibular defects in head-tilt mice result from mutations in Nox3, encoding an NADPH oxidase.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (3) 1 citation
Chemically induced (other) (1)
Targeted (1) 1 citation
Chemically induced (ENU) (3) 1 citation
Endonuclease-mediated (2)

General gene information

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Markers

D17Brg22 (e-PCR), detects polymorphism
- UniSTS:259219

D17Brg23 (e-PCR), detects polymorphism
- UniSTS:259220

Nox3 (e-PCR), detects polymorphism
- UniSTS:525523

NoName (e-PCR)
- UniSTS:224732

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables heme binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables superoxide-generating NAD(P)H oxidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables superoxide-generating NAD(P)H oxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables superoxide-generating NAD(P)H oxidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables superoxide-generating NAD(P)H oxidase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in defense response	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within detection of gravity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within otolith development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to gravity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in superoxide anion generation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within temperature homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of NADPH oxidase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of NADPH oxidase complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: NADPH oxidase 3

Names: head-tilt

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_198958.2 → NP_945196.2 NADPH oxidase 3

See identical proteins and their annotated locations for NP_945196.2

Status: REVIEWED

Source sequence(s)

AC098838, AY573240

Consensus CDS

CCDS28364.2

UniProtKB/Swiss-Prot

Q672J9, Q6Y4Q8

Related

ENSMUSP00000111466.2, ENSMUST00000115800.2

Conserved Domains (2) summary

cd06186
Location:295 → 568

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

pfam01794
Location:66 → 217

Ferric_reduct; Ferric reductase like transmembrane component