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Prkra protein kinase, interferon inducible double stranded RNA dependent activator [ Mus musculus (house mouse) ]

Gene ID: 23992, updated on 21-Apr-2024

Summary

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Official Symbol
Prkraprovided by MGI
Official Full Name
protein kinase, interferon inducible double stranded RNA dependent activatorprovided by MGI
Primary source
MGI:MGI:1344375
See related
Ensembl:ENSMUSG00000002731 AllianceGenome:MGI:1344375
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
PRK; RAX; Pact; lear
Summary
Predicted to enable RNA binding activity; protein homodimerization activity; and protein kinase binding activity. Acts upstream of or within several processes, including cellular response to oxidative stress; ear morphogenesis; and positive regulation of intrinsic apoptotic signaling pathway. Predicted to be located in cytosol and nucleoplasm. Predicted to be part of RISC-loading complex. Is expressed in several structures, including 4-cell stage embryo; central nervous system; olfactory epithelium; and retina. Human ortholog(s) of this gene implicated in dystonia 16. Orthologous to human PRKRA (protein activator of interferon induced protein kinase EIF2AK2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in CNS E18 (RPKM 16.2), CNS E14 (RPKM 14.3) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
2 C3; 2 45.07 cM
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (76436385..76478614, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (76609215..76648220, complement)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene RNA binding motif protein 45 Neighboring gene oxysterol binding protein-like 6 Neighboring gene STARR-seq mESC enhancer starr_04893 Neighboring gene STARR-positive B cell enhancer ABC_E2624 Neighboring gene pejvakin Neighboring gene FK506 binding protein 7

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Prkra Mutation Alters mRNA Expression During Embryonic External Ear Development. Liu W, et al. J Craniofac Surg, 2023 Jun 1. PMID 37185168
  2. Prkra Mutation Alters Long Noncoding RNA Expression During Embryonic External Ear Development. Liu W, et al. J Craniofac Surg, 2023 Mar-Apr 01. PMID 35968982
  3. Point Mutation in Prkra Alters miRNA Expression During Embryonic External Ear Development. Liu W, et al. J Craniofac Surg, 2023 Mar-Apr 01. PMID 35968958
  4. PKR deficiency alters E. coli-induced sickness behaviors but does not exacerbate neuroimmune responses or bacterial load. Poon DC, et al. J Neuroinflammation, 2015 Nov 19. PMID 26585788, Free PMC Article
  5. PACT/RAX regulates the migration of cerebellar granule neurons in the developing cerebellum. Yong Y, et al. Sci Rep, 2015 Jan 22. PMID 25609658, Free PMC Article

See all (55) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Cell-type-specific need of Ddx3 and PACT for interferon induction by RNA viruses.
    Title: Cell-type-specific need of Ddx3 and PACT for interferon induction by RNA viruses.
  2. Prkra Mutation Alters mRNA Expression During Embryonic External Ear Development.
    Title: Prkra Mutation Alters mRNA Expression During Embryonic External Ear Development.
  3. Point Mutation in Prkra Alters miRNA Expression During Embryonic External Ear Development.
    Title: Point Mutation in Prkra Alters miRNA Expression During Embryonic External Ear Development.
  4. Prkra Mutation Alters Long Noncoding RNA Expression During Embryonic External Ear Development.
    Title: Prkra Mutation Alters Long Noncoding RNA Expression During Embryonic External Ear Development.
  5. PACT establishes a posttranscriptional brake on mitochondrial biogenesis by promoting the maturation of miR-181c.
    Title: PACT establishes a posttranscriptional brake on mitochondrial biogenesis by promoting the maturation of miR-181c.
  6. Dicer represses the interferon response and the double-stranded RNA-activated protein kinase pathway in mouse embryonic stem cells.
    Title: Dicer represses the interferon response and the double-stranded RNA-activated protein kinase pathway in mouse embryonic stem cells.
  7. Opposite actions of two dsRNA-binding proteins PACT and TRBP on RIG-I mediated signaling.
    Title: Opposite actions of two dsRNA-binding proteins PACT and TRBP on RIG-I mediated signaling.
  8. A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells.
    Title: A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells.
  9. PACT enhances mammalian embryonic neural stem cell properties as assessed in vitro and in vivo. PACT augments the Notch signaling pathway, a master regulator of neural stem cell fate, at the transcriptional level.
    Title: PACT increases mammalian embryonic neural stem cell properties by facilitating activation of the notch signaling pathway.
  10. TARBP2, but not PRKRA, functions in miRNA biogenesis of a subclass of miRNAs, and suggesting that functional redundancy between TARBP2 and PRKRA does not involve miRNA biogenesis.
    Title: Functional Redundancy of DICER Cofactors TARBP2 and PRKRA During Murine Embryogenesis Does Not Involve miRNA Biogenesis.
Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables double-stranded RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables double-stranded RNA binding ISO
Inferred from Sequence Orthology
more info
  
enables enzyme binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables pre-miRNA binding ISO
Inferred from Sequence Orthology
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables protein kinase activator activity ISO
Inferred from Sequence Orthology
more info
  
enables protein kinase binding ISO
Inferred from Sequence Orthology
more info
  
enables siRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in RISC complex assembly ISO
Inferred from Sequence Orthology
more info
  
involved_in antiviral innate immune response ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within cellular response to oxidative stress IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within ear development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in miRNA processing ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within middle ear morphogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within outer ear morphogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of cell population proliferation ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within positive regulation of intrinsic apoptotic signaling pathway IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in pre-miRNA processing ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within protein phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein stabilization ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of regulatory ncRNA processing IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within regulatory ncRNA-mediated gene silencing IEA
Inferred from Electronic Annotation
more info
  
involved_in siRNA processing IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in siRNA processing ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within skeletal system morphogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of RISC complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of RISC-loading complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of RISC-loading complex ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in intracellular anatomical structure IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
interferon-inducible double-stranded RNA-dependent protein kinase activator A
Names
PKR-associated protein X
PKR-associating protein X
protein activator of the interferon-induced protein kinase

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001421199.1 → NP_001408128.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL928867

  2. NM_001421200.1 → NP_001408129.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 3

    Status: VALIDATED

    Source sequence(s)
    AL928867

  3. NM_001421201.1 → NP_001408130.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 4

    Status: VALIDATED

    Source sequence(s)
    AL928867

  4. NM_001421202.1 → NP_001408131.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 5

    Status: VALIDATED

    Source sequence(s)
    AL928867

  5. NM_001421203.1 → NP_001408132.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 6

    Status: VALIDATED

    Source sequence(s)
    AL928867

  6. NM_001421204.1 → NP_001408133.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 7

    Status: VALIDATED

    Source sequence(s)
    AL928867

  7. NM_001421205.1 → NP_001408134.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 7

    Status: VALIDATED

    Source sequence(s)
    AL928867

  8. NM_001421206.1 → NP_001408135.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 8

    Status: VALIDATED

    Source sequence(s)
    AL928867

  9. NM_001421207.1 → NP_001408136.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 9

    Status: VALIDATED

    Source sequence(s)
    AL928867

  10. NM_011871.4 → NP_036001.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 1

    See identical proteins and their annotated locations for NP_036001.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AL928867
    Consensus CDS
    CCDS16159.1
    UniProtKB/Swiss-Prot
    Q9CZB7, Q9WTX2
    Related
    ENSMUSP00000002808.7, ENSMUST00000002808.7
    Conserved Domains (3) summary
    cd19889
    Location:32 → 102
    DSRM_PRKRA_rpt1; first double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins
    cd19891
    Location:126 → 192
    DSRM_PRKRA_rpt2; second double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins
    cd19892
    Location:239 → 310
    DSRM_PRKRA_rpt3; third double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins

RNA

  1. NR_185269.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL928867

  2. NR_185270.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL928867

  3. NR_185271.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL928867

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    76436385..76478614 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030250935.2 → XP_030106795.1  interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform X2

    Conserved Domains (3) summary
    cd19889
    Location:113 → 183
    DSRM_PRKRA_rpt1; first double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins
    cd19891
    Location:207 → 273
    DSRM_PRKRA_rpt2; second double-stranded RNA binding motif of protein activator of the interferon-induced protein kinase (PRKRA) and similar proteins
    cl00054
    Location:320 → 342
    DSRM_SF; double-stranded RNA binding motif (DSRM) superfamily
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9WTX2.1 GenPept UniProtKB/Swiss-Prot:Q9WTX2

Gene LinkOut

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