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Napepld N-acyl phosphatidylethanolamine phospholipase D [ Mus musculus (house mouse) ]

Gene ID: 242864, updated on 21-Apr-2024

Summary

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Official Symbol
Napepldprovided by MGI
Official Full Name
N-acyl phosphatidylethanolamine phospholipase Dprovided by MGI
Primary source
MGI:MGI:2140885
See related
Ensembl:ENSMUSG00000044968 AllianceGenome:MGI:2140885
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Mbldc1; NAPE-PLD; A530089G06
Summary
Enables N-acylphosphatidylethanolamine-specific phospholipase D activity. Involved in several processes, including N-acylphosphatidylethanolamine metabolic process; host-mediated regulation of intestinal microbiota composition; and positive regulation of brown fat cell differentiation. Acts upstream of or within N-acylethanolamine metabolic process. Predicted to be located in Golgi apparatus. Predicted to be active in cytoplasm and membrane-bounded organelle. Orthologous to human NAPEPLD (N-acyl phosphatidylethanolamine phospholipase D). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in adrenal adult (RPKM 7.3), testis adult (RPKM 5.6) and 27 other tissues See more
Orthologs
human all
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Genomic context

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Location:
5 A3; 5 9.97 cM
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (21867899..21906620, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (21662901..21701622, complement)

Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene F-box and leucine-rich repeat protein 13 Neighboring gene STARR-seq mESC enhancer starr_12568 Neighboring gene STARR-seq mESC enhancer starr_12569 Neighboring gene protein archease pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E11283 Neighboring gene armadillo repeat containing 10 Neighboring gene STARR-seq mESC enhancer starr_12572 Neighboring gene microRNA 12197 Neighboring gene STARR-positive B cell enhancer ABC_E6311 Neighboring gene BCL2-associated transcription factor 1 pseudogene Neighboring gene VISTA enhancer mm1463 Neighboring gene STARR-positive B cell enhancer ABC_E11721 Neighboring gene predicted gene, 54323 Neighboring gene peptidase (mitochondrial processing) beta Neighboring gene predicted gene 15715

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Intestinal NAPE-PLD contributes to short-term regulation of food intake via gut-to-brain axis. Rastelli M, et al. Am J Physiol Endocrinol Metab, 2020 Sep 1. PMID 32776827
  2. Symmetrically substituted dichlorophenes inhibit N-acyl-phosphatidylethanolamine phospholipase D. Aggarwal G, et al. J Biol Chem, 2020 May 22. PMID 32284327, Free PMC Article
  3. A protective role for N-acylphosphatidylethanolamine phospholipase D in 6-OHDA-induced neurodegeneration. Palese F, et al. Sci Rep, 2019 Nov 4. PMID 31685899, Free PMC Article
  4. Intestinal epithelial N-acylphosphatidylethanolamine phospholipase D links dietary fat to metabolic adaptations in obesity and steatosis. Everard A, et al. Nat Commun, 2019 Jan 28. PMID 30692526, Free PMC Article
  5. Peripheral tissue levels and molecular species compositions of N-acyl-phosphatidylethanolamine and its metabolites in mice lacking N-acyl-phosphatidylethanolamine-specific phospholipase D. Inoue M, et al. J Biochem, 2017 Dec 1. PMID 28992041

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. NAPE-specific phospholipase D regulates LRRK2 association with neuronal membranes.
    Title: NAPE-specific phospholipase D regulates LRRK2 association with neuronal membranes.
  2. Hepatic NAPE-PLD Is a Key Regulator of Liver Lipid Metabolism.
    Title: Hepatic NAPE-PLD Is a Key Regulator of Liver Lipid Metabolism.
  3. Intestinal NAPE-PLD contributes to short-term regulation of food intake via gut-to-brain axis.
    Title: Intestinal NAPE-PLD contributes to short-term regulation of food intake via gut-to-brain axis.
  4. Symmetrically substituted dichlorophenes inhibit N-acyl-phosphatidylethanolamine phospholipase D.
    Title: Symmetrically substituted dichlorophenes inhibit N-acyl-phosphatidylethanolamine phospholipase D.
  5. A protective role for N-acylphosphatidylethanolamine phospholipase D in 6-OHDA-induced neurodegeneration.
    Title: A protective role for N-acylphosphatidylethanolamine phospholipase D in 6-OHDA-induced neurodegeneration.
  6. Data indicate that N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) knockout mice showed a broad range of lipids that were differentially affected by lipid species and brain region.
    Title: Lipidomics profile of a NAPE-PLD KO mouse provides evidence of a broader role of this enzyme in lipid metabolism in the brain.
  7. Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota.
    Title: Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota.
  8. Authors demonstrated that phospholipase D and diacylglycerol lipase were required for providing arachidonic acid for prostaglandin E(2) biosynthesis.
    Title: Francisella tularensis LVS induction of prostaglandin biosynthesis by infected macrophages requires specific host phospholipases and lipid phosphatases.
  9. a major physiological role of NAPE-PLD
    Title: Isolevuglandin-modified phosphatidylethanolamine is metabolized by NAPE-hydrolyzing phospholipase D.
  10. Phospholipase D is dispensable for superoxide production and degranulation in mouse neutrophils.
    Title: Molecular mechanisms of N-formyl-methionyl-leucyl-phenylalanine-induced superoxide generation and degranulation in mouse neutrophils: phospholipase D is dispensable.
Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Targeted (5)  1 citation
  • Endonuclease-mediated (2) 

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables N-acetylphosphatidylethanolamine-hydrolysing phospholipase activity IEA
Inferred from Electronic Annotation
more info
  
enables N-acylphosphatidylethanolamine-specific phospholipase D activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables N-acylphosphatidylethanolamine-specific phospholipase D activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables N-acylphosphatidylethanolamine-specific phospholipase D activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables N-acylphosphatidylethanolamine-specific phospholipase D activity ISO
Inferred from Sequence Orthology
more info
  
enables bile acid binding ISO
Inferred from Sequence Orthology
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables phospholipase activity ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in N-acylethanolamine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within N-acylethanolamine metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within N-acylethanolamine metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in N-acylphosphatidylethanolamine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in N-acylphosphatidylethanolamine metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in N-acylphosphatidylethanolamine metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in N-acylphosphatidylethanolamine metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in host-mediated regulation of intestinal microbiota composition IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within lipid catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of eating behavior ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within phospholipid catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in phospholipid metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of brown fat cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of inflammatory response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in temperature homeostasis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endosome IEA
Inferred from Electronic Annotation
more info
  
is_active_in hippocampal mossy fiber to CA3 synapse IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in membrane-bounded organelle IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
is_active_in postsynaptic membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in presynapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in presynaptic membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in smooth endoplasmic reticulum membrane IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
Names
Metallo-beta-lactamase domain containing 1
NAPE-hydrolyzing phospholipase D
NP_001346892.1
NP_001346893.1
NP_001346894.1
NP_001411611.1
NP_848843.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001359963.1 → NP_001346892.1  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform b

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).
    Source sequence(s)
    AC125343, AC161484, AK049135
    Consensus CDS
    CCDS19105.1
    UniProtKB/Swiss-Prot
    Q8BH82
    UniProtKB/TrEMBL
    Q8BLU8
    Related
    ENSMUSP00000110872.2, ENSMUST00000115217.8
    Conserved Domains (1) summary
    cl23716
    Location:129 → 316
    metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain

  2. NM_001359964.1 → NP_001346893.1  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform a

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (a).
    Source sequence(s)
    AC125343, AC161484
    UniProtKB/TrEMBL
    Q8BLU8
    Conserved Domains (1) summary
    cl23716
    Location:137 → 324
    metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain

  3. NM_001359965.1 → NP_001346894.1  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform b

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).
    Source sequence(s)
    AC125343, AC161484
    Consensus CDS
    CCDS19105.1
    UniProtKB/Swiss-Prot
    Q8BH82
    UniProtKB/TrEMBL
    Q8BLU8
    Conserved Domains (1) summary
    cl23716
    Location:129 → 316
    metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain

  4. NM_001424682.1 → NP_001411611.1  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform b

    Status: VALIDATED

    Source sequence(s)
    AC125343, AC161484
    UniProtKB/Swiss-Prot
    Q8BH82

  5. NM_178728.6 → NP_848843.1  N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform b

    See identical proteins and their annotated locations for NP_848843.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).
    Source sequence(s)
    AC125343, AC161484, BC062890
    Consensus CDS
    CCDS19105.1
    UniProtKB/Swiss-Prot
    Q8BH82
    UniProtKB/TrEMBL
    Q8BLU8
    Related
    ENSMUSP00000054458.9, ENSMUST00000060899.9
    Conserved Domains (1) summary
    cl23716
    Location:129 → 316
    metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain

RNA

  1. NR_188845.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC125343, AC161484

  2. NR_188846.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC125343, AC161484

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000071.7 Reference GRCm39 C57BL/6J

    Range
    21867899..21906620 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8BH82.1 GenPept UniProtKB/Swiss-Prot:Q8BH82

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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