Rnf31 ring finger protein 31 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rnf31provided by MGI
Official Full Name: ring finger protein 31provided by MGI
Primary source: MGI:MGI:1934704
See related: Ensembl:ENSMUSG00000047098 AllianceGenome:MGI:1934704
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: HOIP; Paul; Flj10111; mFLJ00217
Summary: Predicted to enable several functions, including linear polyubiquitin binding activity; polyubiquitin modification-dependent protein binding activity; and ubiquitin protein ligase binding activity. Involved in CD40 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB signaling; and protein ubiquitination. Acts upstream of or within negative regulation of necroptotic process. Located in cytoplasmic side of plasma membrane and cytosol. Part of CD40 receptor complex. Is expressed in several structures, including brain; genitourinary system; gut; hemolymphoid system gland; and sensory organ. Orthologous to human RNF31 (ring finger protein 31). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in thymus adult (RPKM 23.8), spleen adult (RPKM 23.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 C3; 14 28.19 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (55829199..55841131)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (55591713..55603674)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.
Title: Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.
The E3 ubiquitin ligase RNF31 mediates the development of ulcerative colitis by regulating NLRP3 inflammasome activation.
Title: The E3 ubiquitin ligase RNF31 mediates the development of ulcerative colitis by regulating NLRP3 inflammasome activation.
Loss of Rnf31 and Vps4b sensitizes pancreatic cancer to T cell-mediated killing.
Title: Loss of Rnf31 and Vps4b sensitizes pancreatic cancer to T cell-mediated killing.
Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling.
Title: Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling.
Deleting TNF receptor 1 could rescue the lethality of RNF31 epidermis-specific KO mice.
Title: RNF31 Regulates Skin Homeostasis by Protecting Epidermal Keratinocytes from Cell Death.
Data show that keratinocyte-specific deletion of ubiquitin-protein ligases HOIP, HOIL-1 (E-KO) and shank-interacting protein-like 1 protein (SHARPIN) results in severe dermatitis causing postnatal lethality.
Title: LUBAC prevents lethal dermatitis by inhibiting cell death induced by TNF, TRAIL and CD95L.
results demonstrate that both HOIP and HOIL-1 are essential LUBAC components and are required for embryogenesis by preventing aberrant cell death; furthermore, they reveal that when LUBAC and caspase-8 are absent, RIPK3 prevents RIPK1 from inducing embryonic lethality by causing defects in fetal haematopoiesis
Title: LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.
SPATA2 has been described as a previously unrecognized factor in the linear ubiquitin chain assembly complex-dependent signaling pathways that serves as an adaptor between HOIP and CYLD, thereby enabling recruitment of CYLD to signaling complexes.
Title: SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.
HOIP's catalytic activity is necessary for preventing TNF-induced cell death. Hence, LUBAC and its linear-ubiquitin-forming activity are required for maintaining vascular integrity during embryogenesis by preventing TNFR1-mediated endothelial cell death.
Title: HOIP deficiency causes embryonic lethality by aberrant TNFR1-mediated endothelial cell death.
Restoration of HOIP expression reversed the defects in cellular activation and signaling. These results reveal HOIP as a key component of the CD40 signaling pathway
Title: HOIL-1L interacting protein (HOIP) is essential for CD40 signaling.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (10) 1 citation
Endonuclease-mediated (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Rnf31 (e-PCR), detects polymorphism
- UniSTS:256489

RH124195 (e-PCR)
- UniSTS:161017

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables K48-linked polyubiquitin modification-dependent protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables K63-linked polyubiquitin modification-dependent protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables linear polyubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in CD40 signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in T cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in defense response to bacterium	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of necroptotic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical NF-kappaB signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of xenophagy	ISO Inferred from Sequence Orthology more info
involved_in protein linear polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein linear polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tumor necrosis factor-mediated signaling pathway	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of CD40 receptor complex	IDA Inferred from Direct Assay more info	PubMed
part_of LUBAC complex	IDA Inferred from Direct Assay more info	PubMed
part_of LUBAC complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase RNF31

Names: HOIL-1-interacting protein; RING-type E3 ubiquitin transferase RNF31; putative Ariadne-like ubiquitin ligase

NP_919327.2

EC 2.3.2.31

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_194346.3 → NP_919327.2 E3 ubiquitin-protein ligase RNF31

See identical proteins and their annotated locations for NP_919327.2

Status: VALIDATED

Source sequence(s)

AC174678, BC057595, BY426496

Consensus CDS

CCDS27118.1

UniProtKB/Swiss-Prot

Q924T7

UniProtKB/TrEMBL

Q6WIZ9

Related

ENSMUSP00000019443.9, ENSMUST00000019443.15

Conserved Domains (6) summary

smart00647
Location:773 → 835

IBR; In Between Ring fingers

cd14325
Location:565 → 619

UBA_RNF31; UBA domain found in E3 ubiquitin-protein ligase RING finger protein 31 and similar proteins

sd00029
Location:348 → 367

zf-RanBP2; RanBP2-type Zn finger [structural motif]

pfam04981
Location:693 → 743

NMD3; NMD3 family

pfam16678
Location:477 → 621

HOIP-UBA; UB-associated-like domain of HOIP or E3 ubiquitin-protein ligase

cl15262
Location:51 → 152

PUB; PNGase/UBA or UBX (PUB) domain of p97 adaptor proteins