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inc insomniac [ Drosophila melanogaster (fruit fly) ]

Gene ID: 31110, updated on 10-May-2024

Summary

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Official Symbol
incprovided by FlyBase
Official Full Name
insomniacprovided by FlyBase
Primary source
FLYBASE:FBgn0025394
Locus tag
Dmel_CG32810
See related
AllianceGenome:FB:FBgn0025394
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG14783; CG14784; CG32810; Dmel\CG32810; EG:196F3.2; INC; ins
Summary
Enables myosin binding activity and protein homodimerization activity. Involved in regulation of circadian sleep/wake cycle, sleep and sleep. Predicted to be part of Cul3-RING ubiquitin ligase complex. Predicted to be active in cytoplasm. Is expressed in several structures, including adult and larval ventral nerve cord. Human ortholog(s) of this gene implicated in myoclonic dystonia 26. Orthologous to several human genes including KCTD5 (potassium channel tetramerization domain containing 5). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs
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Genomic context

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See inc in Genome Data Viewer
Location:
2B1-2B1; 1-0.2 cM
Exon count:
7
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) X NC_004354.4 (1483479..1517615)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) X NC_004354.3 (1377512..1411648)

Chromosome X - NC_004354.4Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene meiotic 38 Neighboring gene ncRNA Neighboring gene NMDA receptor 2 Neighboring gene rush hour Neighboring gene stubarista Neighboring gene uncharacterized protein Neighboring gene Mucin related 2B Neighboring gene small non-coding RNA 400 Neighboring gene broad

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Evaluation of Ligand-Inducible Expression Systems for Conditional Neuronal Manipulations of Sleep in Drosophila. Li Q, et al. G3 (Bethesda), 2016 Oct 13. PMID 27558667, Free PMC Article
  2. Chronic sleep loss disrupts rhythmic gene expression in Drosophila. Wang Z, et al. Front Physiol, 2022. PMID 36467698, Free PMC Article
  3. Sleep Modulates Alcohol Toxicity in Drosophila. De Nobrega AK, et al. Int J Mol Sci, 2022 Oct 11. PMID 36292943, Free PMC Article
  4. insomniac links the development and function of a sleep-regulatory circuit. Li Q, et al. Elife, 2021 Dec 15. PMID 34908527, Free PMC Article
  5. Conserved properties of Drosophila Insomniac link sleep regulation and synaptic function. Li Q, et al. PLoS Genet, 2017 May. PMID 28558011, Free PMC Article

See all (66) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. insomniac links the development and function of a sleep-regulatory circuit.
    Title: insomniac links the development and function of a sleep-regulatory circuit.
  2. Study identified insomniac (inc), a putative adaptor for the Cullin-3 (Cul3) ubiquitin ligase complex, which together with Cul3 is essential for normal sleep regulation. Interestingly, Inc and Cul3 rapidly accumulate at postsynaptic compartments following acute receptor inhibition and are required for a local increase in mono-ubiquitination.
    Title: Cul3 and insomniac are required for rapid ubiquitination of postsynaptic targets and retrograde homeostatic signaling.
  3. inc mutants exhibit defects in synaptic structure and physiology, indicating that Inc is essential for both sleep and synaptic function. Inc and its mouse orthologs exhibit striking biochemical and functional interchangeability within Cul3 complexes.
    Title: Conserved properties of Drosophila Insomniac link sleep regulation and synaptic function.
  4. Data show that insomniac protein (INC) recognize its potential partners Cul3 and dGRASP.
    Title: Proteins involved in sleep homeostasis: Biophysical characterization of INC and its partners.
  5. these results establish inc and Cul3 as important new players in setting the sleep homeostat and a dopaminergic arousal pathway in Drosophila.
    Title: Cul3 and the BTB adaptor insomniac are key regulators of sleep homeostasis and a dopamine arousal pathway in Drosophila.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

mobile_element

  • Loc: 1514190-1514452 mobile_element_type = transposon:1360{}17

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables cullin family protein binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables myosin binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein homooligomerization IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of circadian sleep/wake cycle, sleep IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_positive_effect retrograde trans-synaptic signaling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in sleep IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of Cul3-RING ubiquitin ligase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in postsynaptic cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
insomniac
Names
CG32810-PB
CG32810-PC
CG32810-PD
inc-PB
inc-PC
inc-PD

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_004354.4 Reference assembly

    Range
    1483479..1517615
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001297857.1 → NP_001284786.1  insomniac, isoform D [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001284786.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q9W579
    Conserved Domains (2) summary
    smart00225
    Location:23 → 122
    BTB; Broad-Complex, Tramtrack and Bric a brac
    pfam02214
    Location:24 → 112
    BTB_2; BTB/POZ domain

  2. NM_130570.3 → NP_569926.2  insomniac, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_569926.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q9W579
    Related
    FBpp0070250
    Conserved Domains (2) summary
    smart00225
    Location:23 → 122
    BTB; Broad-Complex, Tramtrack and Bric a brac
    pfam02214
    Location:24 → 112
    BTB_2; BTB/POZ domain

  3. NM_001297858.1 → NP_001284787.1  insomniac, isoform C [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001284787.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q9W579
    Conserved Domains (2) summary
    smart00225
    Location:23 → 122
    BTB; Broad-Complex, Tramtrack and Bric a brac
    pfam02214
    Location:24 → 112
    BTB_2; BTB/POZ domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O76889 O76889
Q8SZH4 Q8SZH4
Q8T9K2 Q8T9K2
Q9W579 Q9W579

Gene LinkOut

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