Met Methoprene-tolerant [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Metprovided by FlyBase
Official Full Name: Methoprene-tolerantprovided by FlyBase
Primary source: FLYBASE:FBgn0002723
Locus tag: Dmel_CG1705
See related: AllianceGenome:FB:FBgn0002723
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: bHLHe59; CG1705; Dmel\CG1705; DmMet; met; MET; Met/Met1; Mett; Rst(1)JH
Summary: Enables nuclear receptor activity and protein heterodimerization activity. Involved in negative regulation of programmed cell death; positive regulation of transcription by RNA polymerase II; and regulation of developmental process. Located in nucleus. Is expressed in several structures, including embryonic/larval gut; gland; larval imaginal tissue; reproductive system; and supraesophageal ganglion. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 10D1-10D1; 1-35 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (11616124..11621280)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (11510157..11515313)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss of function of the miRNA bantam in the fly Drosophila melanogaster increased JHAMT expression, while overexpression of the bantam repressed JHAMT expression and resulted in pupal lethality. In addition, there was a conserved set of miRNAs that interacted with JHAMT, and the sesquiterpenoid receptor methoprene-tolerant (Met).
Title: MicroRNAs regulate the sesquiterpenoid hormonal pathway in Drosophila and other arthropods.
Nup358 facilitates JH-induced Met nuclear transport in a manner dependent on importin beta and Hsp83.
Title: Nucleoporin Nup358 facilitates nuclear import of Methoprene-tolerant (Met) in an importin β- and Hsp83-dependent manner.
the age-related sensitization of Or47b neurons requires a reproductive hormone, juvenile hormone, as well as its binding protein Methoprene-tolerant.
Title: Hormonal Modulation of Pheromone Detection Enhances Male Courtship Success.
genetic evidence definitively establishes Gce/Met in a Juvenile hormone receptor role, thus resolving a long-standing question in arthropod biology.
Title: Genetic Evidence for Function of the bHLH-PAS Protein Gce/Met As a Juvenile Hormone Receptor.
MF is produced by the larval CA and released into the hemolymph, from where it exerts its anti-metamorphic effects indirectly after conversion to JHB3, as well as acting as a hormone itself through the two JH receptors, Met and Gce.
Title: Methyl farnesoate plays a dual role in regulating Drosophila metamorphosis.
a novel NR box enables MET and GCE to interact JH-dependently with the AF2 of FTZ-F1.
Title: The Drosophila juvenile hormone receptor candidates methoprene-tolerant (MET) and germ cell-expressed (GCE) utilize a conserved LIXXL motif to bind the FTZ-F1 nuclear receptor.
The intracellular trafficking of MET seems to be regulated by a rather complicated network of different factors.
Title: Sequences that direct subcellular traffic of the Drosophila methoprene-tolerant protein (MET) are located predominantly in the PAS domains.
Met can sense the juvenile hormone signal through direct, specific binding, thus establishing a unique class of intracellular hormone receptors.
Title: Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant.
Drosophila Met and Gce redundantly transduce JH action to prevent 20E-induced caspase-dependent PCD during larval molts.
Title: Drosophila Met and Gce are partially redundant in transducing juvenile hormone action.
Data show that gce can substitute in vivo for Met, alleviating preadult but not adult phenotypic characters.
Title: Paralogous genes involved in juvenile hormone action in Drosophila melanogaster.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables protein heterodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of programmed cell death	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of developmental process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of aryl hydrocarbon receptor complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Methoprene-tolerant

Names: CG1705-PA; CG1705-PB; Met-PA; Met-PB; Methoprene-tolerant protein; juvenile hormone resistance; methoprene tolerant; methoprene-tolerant; resistance (1) juvenile H; resistance to juvenile hormone

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

11616124..11621280

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001298203.1 → NP_001285132.1 Methoprene-tolerant, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285132.1

Status: REVIEWED

UniProtKB/TrEMBL

O44118, Q9VYW2

Conserved Domains (3) summary

cd00083
Location:38 → 87

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:137 → 186

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam14598
Location:403 → 510

PAS_11; PAS domain
NM_078571.4 → NP_511126.2 Methoprene-tolerant, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_511126.2

Status: REVIEWED

UniProtKB/TrEMBL

O44118, Q9VYW2

Related

FBpp0073368

Conserved Domains (3) summary

cd00083
Location:38 → 87

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:137 → 186

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam14598
Location:403 → 510

PAS_11; PAS domain