Ncc69 sodium chloride cotransporter 69 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Ncc69provided by FlyBase
Official Full Name: sodium chloride cotransporter 69provided by FlyBase
Primary source: FLYBASE:FBgn0036279
Locus tag: Dmel_CG4357
See related: AllianceGenome:FB:FBgn0036279
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BEST:CK01027; CG43567; CG4357; CK01027; Dmel\CG4357; mdcds_25302; ncc69; NCC69; NKCC
Summary: Enables sodium:potassium:chloride symporter activity. Involved in chloride transport and potassium ion transport. Located in plasma membrane. Is expressed in several structures, including CNS glial cell; anterior-posterior subdivision of organism; dorsal median cell; gut section; and presumptive embryonic/larval system. Used to study epilepsy. Human ortholog(s) of this gene implicated in Bartter disease; Bartter disease type 1; Gitelman syndrome; autosomal dominant nonsyndromic deafness 78; and essential hypertension. Orthologous to several human genes including SLC12A1 (solute carrier family 12 member 1) and SLC12A2 (solute carrier family 12 member 2). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 69B3-69B3; 3-38 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (12368646..12391444, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (12361746..12384544, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A Drosophila screen identifies NKCC1 as a modifier of NGLY1 deficiency.
Title: A Drosophila screen identifies NKCC1 as a modifier of NGLY1 deficiency.
The glial sodium-potassium-2-chloride cotransporter is required for synaptic transmission in the Drosophila visual system.
Title: The glial sodium-potassium-2-chloride cotransporter is required for synaptic transmission in the Drosophila visual system.
The Drosophila WNK-SPAK/OSR1-NKCC cascade is an essential molecular pathway for osmoregulation.
Title: Hypotonicity stimulates potassium flux through the WNK-SPAK/OSR1 kinase cascade and the Ncc69 sodium-potassium-2-chloride cotransporter in the Drosophila renal tubule.
Ncc69 plays an important role in transepithelial ion and fluid transport in the fly renal tubule and is a target for regulation in antidiuretic states.
Title: The Drosophila NKCC Ncc69 is required for normal renal tubule function.
This study proposed that normally functioning nerves generate extracellular solutes that are removed by Ncc69 under the control of Fray.
Title: Drosophila glia use a conserved cotransporter mechanism to regulate extracellular volume.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables amino acid transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables chloride:monoatomic cation symporter activity	IEA Inferred from Electronic Annotation more info
enables sodium:potassium:chloride symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables sodium:potassium:chloride symporter activity	IDA Inferred from Direct Assay more info	PubMed
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in amino acid transmembrane transport	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in cell volume homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in chloride ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in chloride transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in chloride transport	IDA Inferred from Direct Assay more info	PubMed
involved_in detection of cold stimulus involved in thermoception	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in detection of temperature stimulus involved in sensory perception of pain	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in monoatomic ion transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion transport	IDA Inferred from Direct Assay more info	PubMed
involved_in potassium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in sodium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: sodium chloride cotransporter 69

Names: CG4357-PA; CG4357-PC; CG4357-PD; CG4357-PE; CG4357-PF; CG4357-PG; Ncc69-PA; Ncc69-PC; Ncc69-PD; Ncc69-PE; Ncc69-PF; Ncc69-PG

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

12368646..12391444 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_140315.4 → NP_648572.1 sodium chloride cotransporter 69, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_648572.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VTW8

Related

FBpp0075719

Conserved Domains (4) summary

TIGR00930
Location:155 → 1171

2a30; K-Cl cotransporter

pfam03522
Location:724 → 1171

SLC12; Solute carrier family 12

pfam08403
Location:144 → 183

AA_permease_N; Amino acid permease N-terminal

cl21473
Location:524 → 682

ArsB_NhaD_permease; Anion permease ArsB/NhaD. These permeases have been shown to translocate sodium, arsenate, antimonite, sulfate and organic anions across biological membranes in all three kingdoms of life. A typical anion permease contains 8-13 transmembrane helices ...
NM_001274828.1 → NP_001261757.1 sodium chloride cotransporter 69, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261757.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PCA7

Conserved Domains (4) summary

TIGR00930
Location:155 → 1191

2a30; K-Cl cotransporter

pfam03522
Location:724 → 1191

SLC12; Solute carrier family 12

pfam08403
Location:144 → 183

AA_permease_N; Amino acid permease N-terminal

cl21473
Location:524 → 682

ArsB_NhaD_permease; Anion permease ArsB/NhaD. These permeases have been shown to translocate sodium, arsenate, antimonite, sulfate and organic anions across biological membranes in all three kingdoms of life. A typical anion permease contains 8-13 transmembrane helices ...
NM_001274829.1 → NP_001261758.1 sodium chloride cotransporter 69, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261758.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PI37

Conserved Domains (4) summary

TIGR00930
Location:155 → 1207

2a30; K-Cl cotransporter

pfam03522
Location:724 → 1207

SLC12; Solute carrier family 12

pfam08403
Location:144 → 183

AA_permease_N; Amino acid permease N-terminal

cl21473
Location:524 → 682

ArsB_NhaD_permease; Anion permease ArsB/NhaD. These permeases have been shown to translocate sodium, arsenate, antimonite, sulfate and organic anions across biological membranes in all three kingdoms of life. A typical anion permease contains 8-13 transmembrane helices ...
NM_206344.2 → NP_996066.1 sodium chloride cotransporter 69, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996066.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VTW8

Conserved Domains (4) summary

TIGR00930
Location:155 → 1171

2a30; K-Cl cotransporter

pfam03522
Location:724 → 1171

SLC12; Solute carrier family 12

pfam08403
Location:144 → 183

AA_permease_N; Amino acid permease N-terminal

cl21473
Location:524 → 682

ArsB_NhaD_permease; Anion permease ArsB/NhaD. These permeases have been shown to translocate sodium, arsenate, antimonite, sulfate and organic anions across biological membranes in all three kingdoms of life. A typical anion permease contains 8-13 transmembrane helices ...
NM_001274827.1 → NP_001261756.1 sodium chloride cotransporter 69, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261756.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PCA7

Conserved Domains (4) summary

TIGR00930
Location:155 → 1191

2a30; K-Cl cotransporter

pfam03522
Location:724 → 1191

SLC12; Solute carrier family 12

pfam08403
Location:144 → 183

AA_permease_N; Amino acid permease N-terminal

cl21473
Location:524 → 682

ArsB_NhaD_permease; Anion permease ArsB/NhaD. These permeases have been shown to translocate sodium, arsenate, antimonite, sulfate and organic anions across biological membranes in all three kingdoms of life. A typical anion permease contains 8-13 transmembrane helices ...
NM_001274830.2 → NP_001261759.1 sodium chloride cotransporter 69, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261759.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PCA7

Conserved Domains (4) summary

TIGR00930
Location:155 → 1191

2a30; K-Cl cotransporter

pfam03522
Location:724 → 1191

SLC12; Solute carrier family 12

pfam08403
Location:144 → 183

AA_permease_N; Amino acid permease N-terminal

cl21473
Location:524 → 682

ArsB_NhaD_permease; Anion permease ArsB/NhaD. These permeases have been shown to translocate sodium, arsenate, antimonite, sulfate and organic anions across biological membranes in all three kingdoms of life. A typical anion permease contains 8-13 transmembrane helices ...