CELA1 chymotrypsin like elastase 1 [Sus scrofa (pig)] - Gene

Summary

Gene symbol: CELA1
Gene description: chymotrypsin like elastase 1
See related: Ensembl:ENSSSCG00000047232
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Sus scrofa
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus
Also known as: ELA1; ELS1; Elastase-1
Expression: Biased expression in ovary (RPKM 10.0) and spleen (RPKM 2.4) See more
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 5

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
106	current	Sscrofa11.1 (GCF_000003025.6)	Unplaced Scaffold	NW_018085018.1 (62822..89758)
105	previous assembly	Sscrofa10.2 (GCF_000003025.5)	5	NC_010447.4 (17250142..17279128, complement)

NW_018085018.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Transcriptome data used for gene annotation
Description: Nine kinds of tissues and a mixture of multiple tissues were sequenced for genome annotation
BioProject: PRJNA311523
Publication: PMID 27646534
Analysis date: Thu Dec 14 17:10:49 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Elastase-mediated membrane fusion of highly pathogenic porcine reproductive and respiratory syndrome virus at host cell surface.
Title: Elastase-mediated membrane fusion of highly pathogenic porcine reproductive and respiratory syndrome virus at host cell surface.
insights into the PPE and HNE inhibition
Title: Polar Desolvation and Position 226 of Pancreatic and Neutrophil Elastases Are Crucial to their Affinity for the Kunitz-Type Inhibitors ShPI-1 and ShPI-1/K13L.
Here, we studied the priming of SARS-CoV S by elastase and show an important role for residue Thr(795) in the S2 domain.
Title: Elastase-mediated activation of the severe acute respiratory syndrome coronavirus spike protein at discrete sites within the S2 domain.
the oxygen of the oxopropyl group of the inhibitor is present as an oxygen anion rather than a hydroxyl group, supporting the role of the "oxyanion hole" in stabilizing the tetrahedral intermediate in ELS1 catalysis
Title: Combined high-resolution neutron and X-ray analysis of inhibited elastase confirms the active-site oxyanion hole but rules against a low-barrier hydrogen bond.
Porcine pancreatic elastase (PPE) was crystallized in complex with a novel inhibitor at pH 5 and X-ray diffraction data were collected at a synchrotron source to 1.66 A.
Title: Crystallization and preliminary diffraction studies of porcine pancreatic elastase in complex with a novel inhibitor.
comparison of x-ray structures of alpha1PI complexes with trypsin, PPE, and human neutrophil elastase
Title: Active site distortion is sufficient for proteinase inhibition by serpins: structure of the covalent complex of alpha1-proteinase inhibitor with porcine pancreatic elastase.
Results describe mapping of the binding surface of porcine pancreatic elastase.
Title: Multiple solvent crystal structures: probing binding sites, plasticity and hydration.
crystal structure of PPE complexed with a novel trimacrocyclic peptide inhibitor, FR901451, was solved at 1.9 A resolution
Title: Structure of the complex of porcine pancreatic elastase with a trimacrocyclic peptide inhibitor FR901451.
The crystal structure of the acyl complex of porcine pancreatic elastase with its peptidyl ester substrate N-acetyl-ala-ala-ala-methyl ester (Ac(Ala)3OMe) has been determined at 2.5 A resolution
Title: Direct crystallographic observation of an acyl-enzyme intermediate in the elastase-catalyzed hydrolysis of a peptidyl ester substrate: Exploiting the "glass transition" in protein dynamics.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RefSeq

Function	Evidence Code	Pubs
enables serine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info	PubMed

Process	Evidence Code	Pubs
involved_in proteolysis	IEA Inferred from Electronic Annotation more info	PubMed

Component	Evidence Code	Pubs
located_in extracellular space	IEA Inferred from Electronic Annotation more info	PubMed

General protein information

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Preferred Names: chymotrypsin-like elastase family member 1

Names: chymotrypsin like elastase family member 1; pancreatic elastase I

NP_998988.1

EC 3.4.21.36

XP_005655631.2

EC 3.4.21.36

XP_020937363.1

EC 3.4.21.36

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_213823.1 → NP_998988.1 chymotrypsin-like elastase family member 1 preproprotein

See identical proteins and their annotated locations for NP_998988.1

Status: VALIDATED

Source sequence(s)

D00160

UniProtKB/TrEMBL

A0A8D0IU75

Conserved Domains (2) summary

smart00020
Location:26 → 259

Tryp_SPc; Trypsin-like serine protease

cd00190
Location:27 → 262

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

RefSeqs of Annotated Genomes: Sus scrofa Annotation Release 106 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference Sscrofa11.1 Primary Assembly

Genomic

NW_018085018.1 Reference Sscrofa11.1 Primary Assembly

Range

62822..89758

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_005655574.3 → XP_005655631.2 chymotrypsin-like elastase family member 1 isoform X1

UniProtKB/TrEMBL

A0A5G2QIP4, A0A8D0IU75

Related

ENSSSCP00000064311.2, ENSSSCT00000069896.2

Conserved Domains (1) summary

cd00190
Location:67 → 302

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...
XM_021081704.1 → XP_020937363.1 chymotrypsin-like elastase family member 1 isoform X2

UniProtKB/Swiss-Prot

P00772, Q29625

UniProtKB/TrEMBL

A0A480DJK8, A0A8D0IU75

Conserved Domains (1) summary

cd00190
Location:27 → 262

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...