Spt5 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Spt5provided by FlyBase
Primary source: FLYBASE:FBgn0040273
Locus tag: Dmel_CG7626
See related: AllianceGenome:FB:FBgn0040273
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG7626; dDSIF; Dmel\CG7626; DSIF; dSpt5; Dspt5; spt5; SPT5
Summary: Enables RNA polymerase II complex binding activity; chromatin binding activity; and mRNA binding activity. Involved in dosage compensation; regulation of transcription, DNA-templated; and transcription elongation from RNA polymerase II promoter. Located in polytene chromosome interband and polytene chromosome puff. Part of DSIF complex. Is expressed in adult head and organism. Orthologous to human SUPT5H (SPT5 homolog, DSIF elongation factor subunit). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 56D5-56D7; 2-89 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (19437483..19441898)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (15324988..15329403)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Spt5 interacts genetically with Myc and is limiting for brain tumor growth in Drosophila.
Title: Spt5 interacts genetically with Myc and is limiting for brain tumor growth in Drosophila.
Mutation in the protein-protein interaction domain (REPO domain) of Pho interferes with the dynamics of its interaction with Spt5. The transcriptional kinetics of the heat shock response is negatively affected by a mutation in the REPO domain of Pho
Title: Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus.
KOW5 is involved in promoter-proximal pausing through contact with the nascent RNA
Title: Identification of Regions in the Spt5 Subunit of DRB Sensitivity-inducing Factor (DSIF) That Are Involved in Promoter-proximal Pausing.
Spt5 interacts both physically and genetically with the Polycomb Group (PcG) protein Pleiohomeotic (Pho)
Title: Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila.
We show that SPT5 interacts directly with MSL1 in vitro and is required downstream of MSL complex recruitment, providing the first mechanistic data corroborating the elongation model of dosage compensation.
Title: Mutations in the transcription elongation factor SPT5 disrupt a reporter for dosage compensation in Drosophila.
Spt5 acts both positively and negatively on transcription in vivo during heat shock induction.
Title: Locus-specific requirements for Spt5 in transcriptional activation and repression in Drosophila.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
contributes_to RNA polymerase II complex binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II complex binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein heterodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription elongation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA-templated transcription, elongation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription, elongation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of DNA-templated transcription, elongation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of DNA-templated transcription elongation	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in sex-chromosome dosage compensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription elongation by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription elongation by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription elongation by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in transcription elongation-coupled chromatin remodeling	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of DSIF complex	IBA Inferred from Biological aspect of Ancestor more info
part_of DSIF complex	IDA Inferred from Direct Assay more info	PubMed
part_of DSIF complex	ISS Inferred from Sequence or Structural Similarity more info
located_in polytene chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in polytene chromosome interband	IDA Inferred from Direct Assay more info	PubMed
located_in polytene chromosome puff	IDA Inferred from Direct Assay more info	PubMed
part_of transcription elongation factor complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: Spt5

Names: CG7626-PA; Spt5-PA; complementation group C

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

19437483..19441898

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_144353.4 → NP_652610.1 Spt5 [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_652610.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8IGQ1, Q8T0P5, Q9V460

Related

FBpp0085716

Conserved Domains (11) summary

PRK08559
Location:212 → 352

nusG; transcription antitermination protein NusG; Validated

cd09888
Location:215 → 302

NGN_Euk; Eukaryotic N-Utilization Substance G (NusG) N-terminal (NGN) domain, including plant KTF1 (KOW domain-containing Transcription Factor 1)

smart01104
Location:812 → 908

CTD; Spt5 C-terminal nonapeptide repeat binding Spt4

cd06081
Location:313 → 350

KOW_Spt5_1; KOW domain of Spt5, repeat 1

cd06082
Location:461 → 511

KOW_Spt5_2; KOW domain of Spt5, repeat 2

cd06083
Location:512 → 562

KOW_Spt5_3; KOW domain of Spt5, repeat 3

cd06084
Location:638 → 680

KOW_Spt5_4; KOW domain of Spt5, repeat 4

cd06085
Location:737 → 788

KOW_Spt5_5; KOW domain of Spt5, repeat 5

cd06086
Location:1020 → 1075

KOW_Spt5_6; KOW domain of Spt5, repeat 6

pfam11942
Location:136 → 208

Spt5_N; Spt5 transcription elongation factor, acidic N-terminal

pfam12815
Location:812 → 870

CTD; Spt5 C-terminal nonapeptide repeat binding Spt4