Spag5 sperm associated antigen 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spag5provided by MGI
Official Full Name: sperm associated antigen 5provided by MGI
Primary source: MGI:MGI:1927470
See related: Ensembl:ENSMUSG00000002055 AllianceGenome:MGI:1927470
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: S17; MAP126; Deepest; Mastrin; D11Bhm180e
Summary: Predicted to enable microtubule binding activity. Acts upstream of or within establishment of spindle orientation. Located in ciliary basal body; ciliary rootlet; and mitotic spindle. Is expressed in several structures, including alimentary system; brain; genitourinary system; integumental system; and sensory organ. Orthologous to human SPAG5 (sperm associated antigen 5). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E11.5 (RPKM 16.1), testis adult (RPKM 15.3) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B5; 11 46.74 cM

Exon count:: 24

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (78192412..78213283)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (78301470..78322457)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss-of-function of Astrin by RNAi and overexpression of the coiled-coil domain results in spindle disorganization, chromosome misalignment and meiosis progression arrest.
Title: Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes.
cloning and genomic structure of mastrin; mastrin protein was demonstrated to localize to mitotic spindles during mitosis[masatrin]
Title: Expression and promoter analysis of mouse mastrin gene.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI874642 (e-PCR)
- UniSTS:180263

D11Bhm49 (e-PCR), detects polymorphism
- UniSTS:141652

D11Bhm50 (e-PCR), detects polymorphism
- UniSTS:141654

D11Seg15 (e-PCR), detects polymorphism
- UniSTS:141789

D11Seg16 (e-PCR), detects polymorphism
- UniSTS:141790

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables microtubule binding	ISO Inferred from Sequence Orthology more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid segregation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intracellular transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of spindle assembly	ISO Inferred from Sequence Orthology more info
involved_in protein localization to centrosome	ISO Inferred from Sequence Orthology more info
involved_in regulation of attachment of spindle microtubules to kinetochore	ISO Inferred from Sequence Orthology more info
involved_in regulation of metaphase plate congression	ISO Inferred from Sequence Orthology more info
involved_in spindle organization	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in centriolar satellite	ISO Inferred from Sequence Orthology more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in ciliary basal body	IDA Inferred from Direct Assay more info	PubMed
located_in ciliary rootlet	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in kinetochore	ISO Inferred from Sequence Orthology more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in microtubule plus-end	ISO Inferred from Sequence Orthology more info
located_in mitotic spindle	IDA Inferred from Direct Assay more info	PubMed
located_in mitotic spindle	ISO Inferred from Sequence Orthology more info
located_in mitotic spindle pole	ISO Inferred from Sequence Orthology more info
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in spindle	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: sperm-associated antigen 5

Names: astrin; microtubule-associated protein; mitotic spindle-associated protein p126

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017407.3 → NP_059103.1 sperm-associated antigen 5

See identical proteins and their annotated locations for NP_059103.1

Status: VALIDATED

Source sequence(s)

AL591070

Consensus CDS

CCDS25098.1

UniProtKB/Swiss-Prot

B1AQC8, Q7TME2, Q8CH61

Related

ENSMUSP00000045286.4, ENSMUST00000045026.4

Conserved Domains (1) summary

COG1196
Location:524 → 1144

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

RNA

NR_156178.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AL591070

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

78192412..78213283

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156853.1 → XP_036012746.1 sperm-associated antigen 5 isoform X1

Conserved Domains (2) summary

COG1196
Location:1 → 568

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:58 → 313

SMC_prok_B; chromosome segregation protein SMC, common bacterial type