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Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 [ Rattus norvegicus (Norway rat) ]

Gene ID: 54283, updated on 11-Apr-2024

Summary

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Symbol
Pfkfb4provided by RGD
Full Name
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4provided by RGD
Primary source
RGD:3310
See related
Ensembl:ENSRNOG00000020656 AllianceGenome:RGD:3310
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary
Enables 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Predicted to be involved in fructose 2,6-bisphosphate metabolic process. Predicted to be active in cytosol. Orthologous to human PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Kidney (RPKM 63.1), Testes (RPKM 43.7) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Pfkfb4 in Genome Data Viewer
Location:
8q32
Exon count:
18
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 8 NC_086026.1 (118522371..118565478)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 8 NC_051343.1 (109643558..109687006)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 8 NC_005107.4 (117733347..117776525)

Chromosome 8 - NC_086026.1Genomic Context describing neighboring genes Neighboring gene collagen type VII alpha 1 chain Neighboring gene microRNA 711 Neighboring gene urocortin 2 Neighboring gene uncharacterized LOC100911671 Neighboring gene uncharacterized LOC134480237 Neighboring gene shisa family member 5

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Crystal structure of the H256A mutant of rat testis fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase. Fructose 6-phosphate in the active site leads to mechanisms for both mutant and wild type bisphosphatase activities. Yuen MH, et al. J Biol Chem, 1999 Jan 22. PMID 9890980
  2. The crystal structure of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies. Hasemann CA, et al. Structure, 1996 Sep 15. PMID 8805587
  3. Molecular cloning of the DNA and expression and characterization of rat testes fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase. Sakata J, et al. J Biol Chem, 1991 Aug 25. PMID 1651918
  4. Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes. Houddane A, et al. Cell Signal, 2017 Jun. PMID 28235572
  5. Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation. Gómez M, et al. Biochem Biophys Res Commun, 2009 Sep 18. PMID 19595670

See all (11) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Increased PFKFB4 expression, but not increased PFKFB3 Ser461 phosphorylation, plays a role in increasing glycolysis in mitogen-stimulated thymocytes.
    Title: Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.
  2. The maximum expression of Pfkfb3 was detected before puberty, while that of Pfkfb4 was observed in adulthood during rat sperm maturation.
    Title: Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables 6-phosphofructo-2-kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 6-phosphofructo-2-kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 6-phosphofructo-2-kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables fructose-2,6-bisphosphate 2-phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in carbohydrate phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in fructose 2,6-bisphosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 2,6-bisphosphate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in fructose metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4-like
6PF-2-K/Fru-2,6-P2ASE testis-type isozyme
6PF-2-K/Fru-2,6-P2ase 4
PFK/FBPase 4
NP_062206.1
XP_006243902.1
XP_008764785.1
XP_038937945.1
XP_038937946.1
XP_038937947.1
XP_063122107.1
XP_063122108.1
XP_063122109.1
XP_063122110.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_019333.1 → NP_062206.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4

    See identical proteins and their annotated locations for NP_062206.1

    Status: PROVISIONAL

    Source sequence(s)
    M64797
    UniProtKB/Swiss-Prot
    P25114
    UniProtKB/TrEMBL
    A6I399, B0ZTI0
    Related
    ENSRNOP00000028039.2, ENSRNOT00000028039.5
    Conserved Domains (2) summary
    pfam00300
    Location:252 → 438
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:30 → 249
    6PF2K; 6-phosphofructo-2-kinase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086026.1 Reference GRCr8

    Range
    118522371..118565478
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006243840.5 → XP_006243902.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X2

    UniProtKB/TrEMBL
    A0A8L2R9R3, B0ZTI0
    Related
    ENSRNOP00000074158.2
    Conserved Domains (3) summary
    pfam00300
    Location:241 → 424
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:24 → 238
    6PF2K; 6-phosphofructo-2-kinase
    pfam13671
    Location:30 → 185
    AAA_33; AAA domain

  2. XM_008766563.4 → XP_008764785.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X1

    See identical proteins and their annotated locations for XP_008764785.1

    UniProtKB/Swiss-Prot
    P25114
    UniProtKB/TrEMBL
    A6I399, B0ZTI0
    Conserved Domains (2) summary
    pfam00300
    Location:252 → 438
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:30 → 249
    6PF2K; 6-phosphofructo-2-kinase

  3. XM_039082018.2 → XP_038937946.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X6

    Conserved Domains (1) summary
    pfam01591
    Location:30 → 212
    6PF2K; 6-phosphofructo-2-kinase

  4. XM_039082019.2 → XP_038937947.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X7

    Conserved Domains (1) summary
    pfam01591
    Location:30 → 211
    6PF2K; 6-phosphofructo-2-kinase

  5. XM_063266037.1 → XP_063122107.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X3

  6. XM_063266038.1 → XP_063122108.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X3

  7. XM_063266039.1 → XP_063122109.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X3

  8. XM_039082017.2 → XP_038937945.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X4

    Conserved Domains (2) summary
    pfam00300
    Location:61 → 247
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    cl17190
    Location:1 → 58
    NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...

  9. XM_063266040.1 → XP_063122110.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X5

RNA

  1. XR_010054022.1 RNA Sequence

  2. XR_010054021.1 RNA Sequence

  3. XR_005487907.2 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25114.3 GenPept UniProtKB/Swiss-Prot:P25114

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