B4galt5 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: B4galt5provided by MGI
Official Full Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5provided by MGI
Primary source: MGI:MGI:1927169
See related: Ensembl:ENSMUSG00000017929 AllianceGenome:MGI:1927169
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 9430078I07Rik
Summary: Enables N-acetyllactosamine synthase activity and UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity. Involved in several processes, including ganglioside biosynthetic process via lactosylceramide; neurogenesis; and positive regulation of embryonic development. Acts upstream of or within poly-N-acetyllactosamine biosynthetic process. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in Golgi apparatus. Is expressed in several structures, including epithelium; genitourinary system; heart; nervous system; and respiratory system. Orthologous to human B4GALT5 (beta-1,4-galactosyltransferase 5). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in small intestine adult (RPKM 13.1), adrenal adult (RPKM 12.5) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 H3; 2 87.22 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (167140364..167191155, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (167298444..167349178, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Downregulation of B4GALT5 attenuates cardiac fibrosis through Lumican and Akt/GSK-3beta/beta-catenin pathway.
Title: Downregulation of B4GALT5 attenuates cardiac fibrosis through Lumican and Akt/GSK-3β/β-catenin pathway.
The expression of Beta4GalT5 gene decreases through reduced expression of the Sp1 gene and protein upon differentiation of pre-adipocytes to adipocytes.
Title: Mechanism for Decreased Gene Expression of β4-Galactosyltransferase 5 upon Differentiation of 3T3-L1 Mouse Preadipocytes to Adipocytes.
Lactosylceramide synthase is encoded by B4galt5 and 6 genes in the central nervous system, and these genes are indispensable for neuronal maturation, perineuronal nets formation, and axonal and myelin formation.
Title: Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice.
These results strongly indicate that the amounts of Lac-Cer synthesized by beta4GalT5 correlate with the tumorigenic potentials of malignantly transformed cells.
Title: Gene expression levels of β4-galactosyltransferase 5 correlate with the tumorigenic potentials of B16-F10 mouse melanoma cells.
Data indicate that mice fed D- threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP), an inhibitor of glucosylceramide synthase and lactosylceramide synthase showed marked reduction in tumor volume.
Title: Use of a glycolipid inhibitor to ameliorate renal cancer in a mouse model.
The expression of the beta-1,4-GalT V gene has been shown to be regulated by transcription factors Sp1 and Ets-1 in cancer cells.(review)
Title: [Regulation of human β-1,4-galactosyltransferase V gene expression in cancer cells].
beta-1,4-galactosyltransferase V is involved in lactosylceramide biosynthesis
Title: Involvement of murine β-1,4-galactosyltransferase V in lactosylceramide biosynthesis.
Loss of beta4Gal-T5 is associated with hematoma and defect in extra-embryonic development.
Title: Beta4-galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development.
The results indicate that the growth is delayed by one to one and half day in B4galt5-/- embryos when compared to B4galt5+/- embryos, which results in early death of the embryos by E10.5, probably due to hematopoietic and/or placental defects.
Title: Early lethality of beta-1,4-galactosyltransferase V-mutant mice by growth retardation.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:225041

NoName (e-PCR)
- UniSTS:490770

AW049941 (e-PCR)
- UniSTS:178775

NoName (e-PCR)
- UniSTS:234609

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables N-acetyllactosamine synthase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity	ISO Inferred from Sequence Orthology more info
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in central nervous system myelination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in central nervous system neuron axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ganglioside biosynthetic process via lactosylceramide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycoprotein biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycosylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in neuron maturation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within poly-N-acetyllactosamine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein glycosylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein stability	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within sphingolipid metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: beta-1,4-galactosyltransferase 5

Names: UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5; b4Gal-T5; beta-1,4-GalT II; beta-1,4-GalTase 5; beta-1,4-galactosyltransferase V; beta4Gal-T5; glucosylceramide beta-1,4-galactosyltransferase; lacCer synthase; lactosylceramide synthase

NP_062809.2

EC 2.4.1.274

XP_036018302.1

EC 2.4.1.274

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019835.2 → NP_062809.2 beta-1,4-galactosyltransferase 5

See identical proteins and their annotated locations for NP_062809.2

Status: VALIDATED

Source sequence(s)

AK151469, AL591854, BE981379, CJ134591

Consensus CDS

CCDS17098.1

UniProtKB/Swiss-Prot

A2A5T7, Q9JMK0

UniProtKB/TrEMBL

Q9QY11

Related

ENSMUSP00000104844.3, ENSMUST00000109221.9

Conserved Domains (1) summary

cd00899
Location:161 → 377

b4GalT; Beta-4-Galactosyltransferase is involved in the formation of the poly-N-acetyllactosamine core structures present in glycoproteins and glycosphingolipids

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

167140364..167191155 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036162409.1 → XP_036018302.1 beta-1,4-galactosyltransferase 5 isoform X1

UniProtKB/TrEMBL

Q9QY11

Conserved Domains (1) summary

cl11394
Location:161 → 336

Glyco_tranf_GTA_type; Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold