U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

DHX33 DEAH-box helicase 33 [ Homo sapiens (human) ]

Gene ID: 56919, updated on 5-Mar-2024

Summary

Go to the top of the page Help
Official Symbol
DHX33provided by HGNC
Official Full Name
DEAH-box helicase 33provided by HGNC
Primary source
HGNC:HGNC:16718
See related
Ensembl:ENSG00000005100 MIM:614405; AllianceGenome:HGNC:16718
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
DDX33
Summary
This gene encodes a member of the DEAD box protein family. The DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
Expression
Ubiquitous expression in brain (RPKM 5.2), appendix (RPKM 3.8) and 25 other tissues See more
Orthologs
mouse all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
17p13.2
Exon count:
12
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 17 NC_000017.11 (5440917..5468982, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 17 NC_060941.1 (5334655..5362725, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (5344237..5372302, complement)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene rabaptin, RAB GTPase binding effector protein 1 Neighboring gene nucleoporin 88 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr17:5316624-5317823 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr17:5322619-5323156 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:5323157-5323694 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8069 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11573 Neighboring gene ReSE screen-validated silencer GRCh37_chr17:5342450-5342632 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8070 Neighboring gene RPA interacting protein Neighboring gene complement C1q binding protein Neighboring gene H3K27ac hESC enhancer GRCh37_chr17:5371354-5371936 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11575 Neighboring gene DHX33 divergent transcript Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11576 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11577 Neighboring gene ReSE screen-validated silencer GRCh37_chr17:5389715-5389882 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11578 Neighboring gene derlin 2 Neighboring gene MIS12 kinetochore complex component

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Systematic analysis of the prognostic and immunological role of DHX33 in pan-cancer. Ding J, et al. Environ Toxicol, 2023 Aug. PMID 37256545
  2. Comprehensive analysis of immune implication and prognostic value of DHX33 in sarcoma. Zhang X, et al. Medicine (Baltimore), 2023 Apr 25. PMID 37115050, Free PMC Article
  3. DHX33 promotes colon cancer development downstream of Wnt signaling. Zhu Y, et al. Gene, 2020 Apr 20. PMID 32004669
  4. The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition. Wang H, et al. Cell Signal, 2019 Feb. PMID 30552990
  5. DHX33 expression is increased in hepatocellular carcinoma and indicates poor prognosis. Tian QH, et al. Biochem Biophys Res Commun, 2016 May 13. PMID 27073163

See all (66) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. RNA helicase DHX33 regulates HMGB family genes in human cancer cells.
    Title: RNA helicase DHX33 regulates HMGB family genes in human cancer cells.
  2. Systematic analysis of the prognostic and immunological role of DHX33 in pan-cancer.
    Title: Systematic analysis of the prognostic and immunological role of DHX33 in pan-cancer.
  3. Comprehensive analysis of immune implication and prognostic value of DHX33 in sarcoma.
    Title: Comprehensive analysis of immune implication and prognostic value of DHX33 in sarcoma.
  4. GSK-3beta phosphorylation of DHX33 leads to its ubiquitination mediated protein degradation.
    Title: GSK-3β phosphorylation of DHX33 leads to its ubiquitination mediated protein degradation.
  5. Long non-coding RNA HOTAIR promotes hepatocellular carcinoma progression by regulating miR-526b-3p/DHX33 axis.
    Title: Long non-coding RNA HOTAIR promotes hepatocellular carcinoma progression by regulating miR-526b-3p/DHX33 axis.
  6. Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
    Title: Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
  7. DHX33 Recruits Gadd45a To Cause DNA Demethylation and Regulates a Subset of Gene Transcription.
    Title: DHX33 Recruits Gadd45a To Cause DNA Demethylation and Regulates a Subset of Gene Transcription.
  8. This study highlights that DHX33 protein is critical in glioblastoma cell proliferation and its potential as a novel therapeutic drug target.
    Title: The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition.
  9. DHX33 is highly expressed in human colon cancers. DHX33 and Wnt/beta-catenin reciprocally regulate each other.
    Title: DHX33 promotes colon cancer development downstream of Wnt signaling.
  10. Results reveal that DHX33 represses apoptosis through the direct upregulation of Bcl-2 gene transcription. AP-2beta is a binding partner for DHX33 and that DHX33 acts as a coactivator for AP-2beta to promote the transcription of antiapoptotic Bcl-2 gene.
    Title: DHX33 Interacts with AP-2β To Regulate Bcl-2 Gene Expression and Promote Cancer Cell Survival.
Submit: New GeneRIF Correction See all GeneRIFs (21)

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

Go to the top of the page Help

Replication interactions

Interaction Pubs
Knockdown of DEAH (Asp-Glu-Ala-His) box polypeptide 33 (DHX33) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed

Go to the HIV-1, Human Interaction Database

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • FLJ21972, DKFZp762F2011

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding HDA PubMed 
enables RNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables double-stranded RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables double-stranded RNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables mRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables rDNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribosomal large subunit binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in positive regulation of MAPK cascade ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in positive regulation of NF-kappaB transcription factor activity ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in positive regulation of NLRP3 inflammasome complex assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase I IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of transcription by RNA polymerase I IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of type I interferon production ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in translational initiation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of NLRP3 inflammasome complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in intracellular anatomical structure IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in nucleolus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
ATP-dependent RNA helicase DHX33
Names
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 33
DEAH (Asp-Glu-Ala-His) box polypeptide 33
DEAH box protein 33
putative ATP-dependent RNA helicase DHX33
NP_001186628.1
NP_064547.2
XP_016880366.1
XP_047292374.1
XP_054172671.1
XP_054172672.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001199699.2 → NP_001186628.1  ATP-dependent RNA helicase DHX33 isoform 2

    See identical proteins and their annotated locations for NP_001186628.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an exon in the 5' coding region and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus, compared to isoform 1.
    Source sequence(s)
    AC004148, AK295760, BC030017, BQ775963
    UniProtKB/TrEMBL
    I3L1L6
    Related
    ENSP00000458925.1, ENST00000572490.1
    Conserved Domains (5) summary
    smart00847
    Location:305 → 390
    HA2; Helicase associated domain (HA2) Add an annotation
    COG1643
    Location:1 → 522
    HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
    cd00046
    Location:1 → 61
    DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
    pfam00271
    Location:118 → 235
    Helicase_C; Helicase conserved C-terminal domain
    pfam07717
    Location:424 → 524
    OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold

  2. NM_020162.4 → NP_064547.2  ATP-dependent RNA helicase DHX33 isoform 1

    See identical proteins and their annotated locations for NP_064547.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC004148, AK295760, BQ775963
    Consensus CDS
    CCDS11072.1
    UniProtKB/Swiss-Prot
    B4DHF9, Q4G149, Q5CZ73, Q9H5M9, Q9H6R0
    UniProtKB/TrEMBL
    B4DIS6
    Related
    ENSP00000225296.3, ENST00000225296.8
    Conserved Domains (5) summary
    smart00847
    Location:478 → 563
    HA2; Helicase associated domain (HA2) Add an annotation
    COG1643
    Location:67 → 695
    HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
    cd00046
    Location:92 → 234
    DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
    pfam00271
    Location:291 → 408
    Helicase_C; Helicase conserved C-terminal domain
    pfam07717
    Location:597 → 697
    OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000017.11 Reference GRCh38.p14 Primary Assembly

    Range
    5440917..5468982 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017024877.2 → XP_016880366.1  ATP-dependent RNA helicase DHX33 isoform X2

    UniProtKB/Swiss-Prot
    Q9H6R0
    Conserved Domains (1) summary
    COG1643
    Location:1 → 267
    HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]

  2. XM_047436418.1 → XP_047292374.1  ATP-dependent RNA helicase DHX33 isoform X1

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060941.1 Alternate T2T-CHM13v2.0

    Range
    5334655..5362725 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054316697.1 → XP_054172672.1  ATP-dependent RNA helicase DHX33 isoform X2

  2. XM_054316696.1 → XP_054172671.1  ATP-dependent RNA helicase DHX33 isoform X1

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9H6R0.2 GenPept UniProtKB/Swiss-Prot:Q9H6R0

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous