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Fhl5 four and a half LIM domains 5 [ Mus musculus (house mouse) ]

Gene ID: 57756, updated on 11-Apr-2024

Summary

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Official Symbol
Fhl5provided by MGI
Official Full Name
four and a half LIM domains 5provided by MGI
Primary source
MGI:MGI:1913192
See related
Ensembl:ENSMUSG00000028259 AllianceGenome:MGI:1913192
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Act; 1700027G07Rik
Summary
Enables transcription coactivator activity. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Predicted to be active in Z disc and nucleus. Is expressed in genitourinary system; heart; and otocyst. Orthologous to human FHL5 (four and a half LIM domains 5). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Restricted expression toward testis adult (RPKM 61.6) See more
Orthologs
human all
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Genomic context

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Location:
4 A3; 4 10.58 cM
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (25199907..25242852, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (25199907..25242852, complement)

Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 46848 Neighboring gene NADH:ubiquinone oxidoreductase complex assembly factor 4 Neighboring gene RIKEN cDNA C230012O17 gene Neighboring gene G protein-coupled receptor 63 Neighboring gene UFM1 specific ligase 1 Neighboring gene peroxiredoxin 6 pseudogene 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression. Lardenois A, et al. Reprod Biol Endocrinol, 2009 Nov 24. PMID 19930692, Free PMC Article
  2. Abnormal sperm in mice with targeted deletion of the act (activator of cAMP-responsive element modulator in testis) gene. Kotaja N, et al. Proc Natl Acad Sci U S A, 2004 Jul 20. PMID 15247423, Free PMC Article
  3. Cloning and expression of activator of CREM in testis in human testicular tissue. Palermo I, et al. Biochem Biophys Res Commun, 2001 May 4. PMID 11327716
  4. CREM-dependent transcription in male germ cells controlled by a kinesin. Macho B, et al. Science, 2002 Dec 20. PMID 12493914
  5. CBP-independent activation of CREM and CREB by the LIM-only protein ACT. Fimia GM, et al. Nature, 1999 Mar 11. PMID 10086359

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis.
    Title: Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
  2. Fhl5 is not essential for testicular gene expression to a level detected by microarray-based measurements.
    Title: Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.
  3. Results indicate that numerous postmeiotic genes under CREM (cAMP-responsive element modulator) control require the coactivator function of ACT (activator of CREM) in testis.
    Title: Abnormal sperm in mice with targeted deletion of the act (activator of cAMP-responsive element modulator in testis) gene.
  4. ACT selectively associates with KIF17b, a kinesin highly expressed in male germ cells; The ACT-KIF17b interaction is restricted to specific stages of spermatogenesis and directly determines the intracellular localization of ACT
    Title: CREM-dependent transcription in male germ cells controlled by a kinesin.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1)  1 citation
  • Endonuclease-mediated (2) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transcription coactivator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transcription coactivator activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA-templated transcription TAS
Traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
is_active_in Z disc IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
four and a half LIM domains protein 5
Names
FHL-5
LIM-only protein ACT
activator of CREM in testis
activator of cAMP-responsive element modulator in testis

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001355498.2 → NP_001342427.1  four and a half LIM domains protein 5

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) encodes the same protein as variants 1 and 3.
    Source sequence(s)
    AL772199, AL935267
    Consensus CDS
    CCDS18009.1
    UniProtKB/Swiss-Prot
    Q9WTX7
    Conserved Domains (4) summary
    cd09343
    Location:37 → 95
    LIM1_FHL; The first LIM domain of Four and a half LIM domains protein (FHL)
    cd09347
    Location:222 → 277
    LIM4_FHL; The fourth LIM domain of Four and a half LIM domains protein (FHL)
    cd09428
    Location:102 → 155
    LIM2_FHL5; The second LIM domain of Four and a half LIM domains protein 5 (FHL5)
    cl02475
    Location:163 → 214
    LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

  2. NM_001379528.1 → NP_001366457.1  four and a half LIM domains protein 5

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) represents the longest transcript. All three variants encode the same protein.
    Source sequence(s)
    AL772199, AL935267
    UniProtKB/Swiss-Prot
    Q9WTX7
    Related
    ENSMUSP00000103839.2, ENSMUST00000108204.2
    Conserved Domains (4) summary
    cd09343
    Location:37 → 95
    LIM1_FHL; The first LIM domain of Four and a half LIM domains protein (FHL)
    cd09347
    Location:222 → 277
    LIM4_FHL; The fourth LIM domain of Four and a half LIM domains protein (FHL)
    cd09428
    Location:102 → 155
    LIM2_FHL5; The second LIM domain of Four and a half LIM domains protein 5 (FHL5)
    cl02475
    Location:163 → 214
    LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

  3. NM_021318.5 → NP_067293.1  four and a half LIM domains protein 5

    See identical proteins and their annotated locations for NP_067293.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3.
    Source sequence(s)
    AL772199, AL935267
    Consensus CDS
    CCDS18009.1
    UniProtKB/Swiss-Prot
    Q9WTX7
    Related
    ENSMUSP00000029922.8, ENSMUST00000029922.14
    Conserved Domains (4) summary
    cd09343
    Location:37 → 95
    LIM1_FHL; The first LIM domain of Four and a half LIM domains protein (FHL)
    cd09347
    Location:222 → 277
    LIM4_FHL; The fourth LIM domain of Four and a half LIM domains protein (FHL)
    cd09428
    Location:102 → 155
    LIM2_FHL5; The second LIM domain of Four and a half LIM domains protein 5 (FHL5)
    cl02475
    Location:163 → 214
    LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000070.7 Reference GRCm39 C57BL/6J

    Range
    25199907..25242852 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9WTX7.1 GenPept UniProtKB/Swiss-Prot:Q9WTX7

Gene LinkOut

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