Med12 mediator complex subunit 12 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Med12provided by MGI
Official Full Name: mediator complex subunit 12provided by MGI
Primary source: MGI:MGI:1926212
See related: Ensembl:ENSMUSG00000079487 AllianceGenome:MGI:1926212
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mopa; OPA-1; 230kDa; Tnrc11; Trap230
Summary: Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; chromatin binding activity; and transcription coactivator activity. Acts upstream of or within several processes, including Wnt signaling pathway; chordate embryonic development; and glial cell development. Part of mediator complex. Is expressed in brain. Human ortholog(s) of this gene implicated in FG syndrome; leiomyosarcoma; major depressive disorder; phobic disorder; and syndromic X-linked intellectual disorder Lujan-Fryns-type. Orthologous to human MED12 (mediator complex subunit 12). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in thymus adult (RPKM 22.1), spleen adult (RPKM 17.5) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: X D; X 43.91 cM

Exon count:: 45

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	X	NC_000086.8 (100317697..100342540)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	X	NC_000086.7 (101274091..101298934)

Chromosome X - NC_000086.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Mediator subunit MED12 promotes formation of HSF1 condensates on heat shock response element arrays in heat-shocked cells.
Title: The Mediator subunit MED12 promotes formation of HSF1 condensates on heat shock response element arrays in heat-shocked cells.
MED12 Regulates Human Adipose-Derived Stem Cell Adipogenesis and Mediator Kinase Subunit Expression in Murine Adipose Depots.
Title: MED12 Regulates Human Adipose-Derived Stem Cell Adipogenesis and Mediator Kinase Subunit Expression in Murine Adipose Depots.
MED12 and BRD4 cooperate to sustain cancer growth upon loss of mediator kinase.
Title: MED12 and BRD4 cooperate to sustain cancer growth upon loss of mediator kinase.
Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis.
Title: Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis.
Med12 is critically important in reproductive tract development, and Med12 loss of function does not cause tumorigenesis in reproductive tissues.
Title: Med12 regulates ovarian steroidogenesis, uterine development and maternal effects in the mammalian egg.
Study provide evidence that Med12 regulates neural stem cell gene expression programs linked to adhesion and cell cycle progression but suppresses its adhesion by repressing the expression of cell adhesion molecules.
Title: Mediator subunit Med12 contributes to the maintenance of neural stem cell identity.
alterations in MED12-dependent enhancer regulation may control both physiological and malignant hematopoiesis.
Title: MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis.
The data reveal how the interplay between PRC1, ncRNA and Mediator complexes controls pluripotency and cellular differentiation.
Title: Dual role of Med12 in PRC1-dependent gene repression and ncRNA-mediated transcriptional activation.
Med1 and Med12, two subunits of the mediator complex implicated in transcription initiation and long-range enhancer/promoter loop formation, are dynamically recruited to the IgH locus enhancers.
Title: Mediator facilitates transcriptional activation and dynamic long-range contacts at the IgH locus during class switch recombination.
leiomyomatous, Med12 c.131G>A variant-expressing uteri developed chromosomal rearrangements.
Title: Med12 gain-of-function mutation causes leiomyomas and genomic instability.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

px-6d9 (e-PCR)
- UniSTS:258451

RH79662 (e-PCR)
- UniSTS:83609

M16362 (e-PCR)
- UniSTS:124923

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables beta-catenin binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables nuclear thyroid hormone receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coactivator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription coactivator activity	IGI Inferred from Genetic Interaction more info	PubMed
enables transcription coactivator activity	ISO Inferred from Sequence Orthology more info
enables transcription coregulator activity	ISO Inferred from Sequence Orthology more info
enables transcription factor binding	IBA Inferred from Biological aspect of Ancestor more info
enables transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
contributes_to ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within Schwann cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within Wnt signaling pathway, planar cell polarity pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within axis elongation involved in somitogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic brain development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic neurocranium morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic organ development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endoderm development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neural tube closure	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neural tube development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within oligodendrocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription initiation by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within post-anal tail morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within somatic stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within somitogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spinal cord development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of CKM complex	ISO Inferred from Sequence Orthology more info
part_of mediator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of mediator complex	IDA Inferred from Direct Assay more info	PubMed
part_of mediator complex	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: mediator of RNA polymerase II transcription subunit 12

Names: OPA-containing protein 1; mediator of RNA polymerase II transcription, subunit 12 homolog; thyroid hormone receptor-associated protein complex 230 kDa component; trinucleotide repeat containing 11 (THR-associated protein); trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); trinucleotide repeat-containing gene 11 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_021521.2 → NP_067496.2 mediator of RNA polymerase II transcription subunit 12

See identical proteins and their annotated locations for NP_067496.2

Status: VALIDATED

Source sequence(s)

BC057119, BC090654, BC110696

Consensus CDS

CCDS41078.1

UniProtKB/Swiss-Prot

A2AGH6, A2AGH7, O88542, Q571H3, Q6PGB4, Q6PGD8

UniProtKB/TrEMBL

A2AGH9

Related

ENSMUSP00000085260.5, ENSMUST00000087948.11

Conserved Domains (3) summary

pfam09497
Location:103 → 161

Med12; Transcription mediator complex subunit Med12

pfam12144
Location:1822 → 2024

Med12-PQL; Eukaryotic Mediator 12 catenin-binding domain

pfam12145
Location:287 → 758

Med12-LCEWAV; Eukaryotic Mediator 12 subunit domain