Unc13a unc-13 homolog A [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Unc13aprovided by RGD
Official Full Name: unc-13 homolog Aprovided by RGD
Primary source: RGD:619722
See related: Ensembl:ENSRNOG00000018452 AllianceGenome:RGD:619722
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Unc13h1; Munc13-1
Summary: Enables several functions, including calmodulin binding activity; spectrin binding activity; and syntaxin-1 binding activity. Involved in long-term synaptic potentiation; regulated exocytosis; and regulation of synaptic vesicle priming. Located in several cellular components, including Golgi-associated vesicle; presynaptic active zone cytoplasmic component; and presynaptic membrane. Part of protein-containing complex. Human ortholog(s) of this gene implicated in amyotrophic lateral sclerosis. Orthologous to human UNC13A (unc-13 homolog A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 166.7), Adrenal (RPKM 44.9) and 5 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 16p14

Exon count:: 46

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	16	NC_086034.1 (18367891..18415808, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	16	NC_051351.1 (18333910..18381813, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	16	NC_005115.4 (20056398..20103951, complement)

Chromosome 16 - NC_086034.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Vesicle capture by membrane-bound Munc13-1 requires self-assembly into discrete clusters.
Title: Vesicle capture by membrane-bound Munc13-1 requires self-assembly into discrete clusters.
Synaptotagmin-1-, Munc18-1-, and Munc13-1-dependent liposome fusion with a few neuronal SNAREs.
Title: Synaptotagmin-1-, Munc18-1-, and Munc13-1-dependent liposome fusion with a few neuronal SNAREs.
Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.
Title: Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.
Re-examining how Munc13-1 facilitates opening of syntaxin-1.
Title: Re-examining how Munc13-1 facilitates opening of syntaxin-1.
This study showed that the presynaptic terminal, Munc13-1 molecules form multiple and discrete supramolecular self-assemblies that serve as independent vesicular release sites by recruiting syntaxin-1 essential for synaptic vesicle exocytosis.
Title: Synaptic weight set by Munc13-1 supramolecular assemblies.
Study data suggest that Munc18-1 and Munc13-1 together serve as a functional template to orchestrate SNARE complex assembly.
Title: Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly.
These results suggested that Munc13-1 has an inhibitory role in antigen-induced mast cell degranulation, which is performed in a Munc13-4-dependent manner.
Title: Inhibitory role of Munc13-1 in antigen-induced mast cell degranulation.
W22A, W22K, W22D, W22Y, and W22F substitutions were made in Munc13-1. The GFP-tagged constructs were expressed in Neuro-2a cells. Their membrane translocation in response to phorbol ester was observed in live cells by confocal microscopy. Munc13-1 translocated to the plasma membrane, the C1 domain translocated to internal membranes in response to phorbol ester. Trp-588 is important for ligand binding and translocation.
Title: Critical Role of Trp-588 of Presynaptic Munc13-1 for Ligand Binding and Membrane Translocation.
Together with previous studies, these data support a model whereby Munc18-1 acts as a template for SNARE complex assembly, and autoinhibition of synaptobrevin binding contributes to enabling regulation of neurotransmitter release by Munc13-1.
Title: Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion.
The structure imposes key constraints for models of neurotransmitter release and suggests that Munc13-1 bridges the vesicle and plasma membranes from the periphery of the membrane-membrane interface.
Title: Mechanistic insights into neurotransmitter release and presynaptic plasticity from the crystal structure of Munc13-1 C(1)C(2)BMUN.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BF416213 (e-PCR)
- UniSTS:249237

BE096312 (e-PCR)
- UniSTS:260536

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables SNARE binding	IDA Inferred from Direct Assay more info	PubMed
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin binding	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables diacylglycerol binding	IEA Inferred from Electronic Annotation more info
enables diacylglycerol binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables phospholipid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IDA Inferred from Direct Assay more info	PubMed
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables spectrin binding	IPI Inferred from Physical Interaction more info	PubMed
enables syntaxin binding	IDA Inferred from Direct Assay more info	PubMed
enables syntaxin-1 binding	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin-1 binding	IDA Inferred from Direct Assay more info	PubMed
enables syntaxin-1 binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within amyloid-beta metabolic process	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
NOT involved_in dense core granule docking	ISO Inferred from Sequence Orthology more info
involved_in dense core granule priming	IBA Inferred from Biological aspect of Ancestor more info
involved_in dense core granule priming	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within innervation	ISO Inferred from Sequence Orthology more info
involved_in long-term synaptic potentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in modulation of chemical synaptic transmission	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuromuscular junction development	ISO Inferred from Sequence Orthology more info
involved_in neuronal dense core vesicle exocytosis	ISO Inferred from Sequence Orthology more info
involved_in neuronal dense core vesicle exocytosis	ISS Inferred from Sequence or Structural Similarity more info
involved_in neurotransmitter secretion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of neurotransmitter secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dendrite extension	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glutamate receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of neurotransmitter secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of synaptic plasticity	ISO Inferred from Sequence Orthology more info
involved_in presynaptic dense core vesicle exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in presynaptic dense core vesicle exocytosis	ISO Inferred from Sequence Orthology more info
involved_in regulation of amyloid precursor protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of short-term neuronal synaptic plasticity	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic vesicle priming	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic transmission, glutamatergic	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within synaptic transmission, glutamatergic	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle docking	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within synaptic vesicle docking	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle exocytosis	IEA Inferred from Electronic Annotation more info
involved_in synaptic vesicle exocytosis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within synaptic vesicle maturation	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle priming	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic vesicle priming	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle priming	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi-associated vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in axon	ISO Inferred from Sequence Orthology more info
is_active_in calyx of Held	ISO Inferred from Sequence Orthology more info
located_in excitatory synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
is_active_in neuromuscular junction	IBA Inferred from Biological aspect of Ancestor more info
located_in neuromuscular junction	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in presynapse	IEA Inferred from Electronic Annotation more info
located_in presynapse	ISO Inferred from Sequence Orthology more info
located_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic active zone	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic active zone cytoplasmic component	IBA Inferred from Biological aspect of Ancestor more info
located_in presynaptic active zone cytoplasmic component	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	ISO Inferred from Sequence Orthology more info
located_in synaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in synaptic vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in terminal bouton	HDA more info	PubMed
is_active_in terminal bouton	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: protein unc-13 homolog A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022861.2 → NP_074052.2 protein unc-13 homolog A

Status: VALIDATED

Source sequence(s)

JAXUCZ010000016

UniProtKB/Swiss-Prot

Q62768

UniProtKB/TrEMBL

A0A8I5ZWH4

Related

ENSRNOP00000083088.1, ENSRNOT00000098794.1

Conserved Domains (4) summary

cd04027
Location:689 → 815

C2B_Munc13; C2 domain second repeat in Munc13 (mammalian uncoordinated) proteins

cd08394
Location:1 → 127

C2A_Munc13; C2 domain first repeat in Munc13 (mammalian uncoordinated) proteins

cd08395
Location:1578 → 1697

C2C_Munc13; C2 domain third repeat in Munc13 (mammalian uncoordinated) proteins

smart00109
Location:567 → 616

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086034.1 Reference GRCr8

Range

18367891..18415808 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063275701.1 → XP_063131771.1 protein unc-13 homolog A isoform X7

UniProtKB/TrEMBL

A0A8I6A8K9
XM_063275696.1 → XP_063131766.1 protein unc-13 homolog A isoform X3

UniProtKB/Swiss-Prot

Q62768
XM_063275698.1 → XP_063131768.1 protein unc-13 homolog A isoform X5

UniProtKB/TrEMBL

A0A8I6A9C4
XM_063275695.1 → XP_063131765.1 protein unc-13 homolog A isoform X2

UniProtKB/Swiss-Prot

Q62768
XM_063275700.1 → XP_063131770.1 protein unc-13 homolog A isoform X6

UniProtKB/Swiss-Prot

Q62768
XM_063275694.1 → XP_063131764.1 protein unc-13 homolog A isoform X1

UniProtKB/Swiss-Prot

Q62768
XM_063275702.1 → XP_063131772.1 protein unc-13 homolog A isoform X8

UniProtKB/TrEMBL

A0A8I6A8K9
XM_063275697.1 → XP_063131767.1 protein unc-13 homolog A isoform X4

UniProtKB/TrEMBL

A0A8I6A9C4
XM_063275704.1 → XP_063131774.1 protein unc-13 homolog A isoform X10

UniProtKB/Swiss-Prot

Q62768
XM_063275705.1 → XP_063131775.1 protein unc-13 homolog A isoform X11
XM_063275706.1 → XP_063131776.1 protein unc-13 homolog A isoform X12
XM_063275703.1 → XP_063131773.1 protein unc-13 homolog A isoform X9

UniProtKB/TrEMBL

A0A8I6A8K9