U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Spc25 SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) [ Mus musculus (house mouse) ]

Gene ID: 66442, updated on 5-Mar-2024

Summary

Go to the top of the page Help
Official Symbol
Spc25provided by MGI
Official Full Name
SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)provided by MGI
Primary source
MGI:MGI:1913692
See related
Ensembl:ENSMUSG00000005233 AllianceGenome:MGI:1913692
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Spbc25; 2600017H08Rik; 2610205L13Rik
Summary
This gene encodes a component of the kinetochore-associated NDC80 protein complex, which is required for the mitotic spindle checkpoint and for microtubule-kinetochore attachment. During meiosis in mouse, the protein localizes to the germinal vesicle and then is associated with the chromosomes following germinal vesicle breakdown. Knockdown of this gene in oocytes results in precocious polar body extrusion, chromosome misalignment and aberrant spindle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Expression
Biased expression in liver E14 (RPKM 25.3), CNS E11.5 (RPKM 24.7) and 10 other tissues See more
Orthologs
human all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
2 C2; 2 39.64 cM
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (69009753..69036583, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (69193895..69206213, complement)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene ceramide synthase 6 Neighboring gene predicted gene, 52515 Neighboring gene STARR-seq mESC enhancer starr_04722 Neighboring gene STARR-seq mESC enhancer starr_04727 Neighboring gene predicted gene, 39840 Neighboring gene nitric oxide synthase trafficker Neighboring gene STARR-positive B cell enhancer ABC_E412 Neighboring gene STARR-seq mESC enhancer starr_04729 Neighboring gene glucose-6-phosphatase, catalytic, 2 Neighboring gene STARR-seq mESC enhancer starr_04731 Neighboring gene ATP-binding cassette, sub-family B member 11 Neighboring gene STARR-seq mESC enhancer starr_04732

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Microtubule attachment and spindle assembly checkpoint signalling at the kinetochore. Foley EA, et al. Nat Rev Mol Cell Biol, 2013 Jan. PMID 23258294, Free PMC Article
  2. Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment and spindle assembly checkpoint in mouse oocytes. Sun SC, et al. Cell Cycle, 2010 Nov 15. PMID 21084868
  3. Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex. Ciferri C, et al. Cell, 2008 May 2. PMID 18455984, Free PMC Article
  4. BCAS2 is involved in alternative splicing and mouse oocyte development. Zhang J, et al. FASEB J, 2022 Feb. PMID 34972250
  5. The KMN protein network--chief conductors of the kinetochore orchestra. Varma D, et al. J Cell Sci, 2012 Dec 15. PMID 23418356, Free PMC Article

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Spc25 is required for chromosome alignment, spindle formation, and proper spindle checkpoint signaling during meiosis.
    Title: Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment and spindle assembly checkpoint in mouse oocytes.
Submit: New GeneRIF Correction

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (2) 

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in attachment of spindle microtubules to kinetochore ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within cell cycle IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell division IEA
Inferred from Electronic Annotation
more info
  
involved_in chromosome segregation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in chromosome segregation ISO
Inferred from Sequence Orthology
more info
  
involved_in chromosome segregation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in mitotic spindle assembly checkpoint signaling NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in mitotic spindle organization ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of Ndc80 complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of Ndc80 complex ISO
Inferred from Sequence Orthology
more info
  
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in chromosome, centromeric region IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in kinetochore ISO
Inferred from Sequence Orthology
more info
  
located_in kinetochore NAS
Non-traceable Author Statement
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
kinetochore protein Spc25
Names
spindle pole body component 25 homolog

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001199123.2 → NP_001186052.1  kinetochore protein Spc25 isoform 1

    See identical proteins and their annotated locations for NP_001186052.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 2, and 3 encode the same isoform.
    Source sequence(s)
    AL929221, BC033605, CJ086993
    Consensus CDS
    CCDS57174.1
    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Related
    ENSMUSP00000107939.2, ENSMUST00000112320.8
    Conserved Domains (2) summary
    COG4942
    Location:55 → 129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151 → 220
    Spindle_Spc25; Chromosome segregation protein Spc25

  2. NM_001199124.2 → NP_001186053.1  kinetochore protein Spc25 isoform 1

    See identical proteins and their annotated locations for NP_001186053.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1).
    Source sequence(s)
    AL929221, BC027121, CJ086993, CX222514
    Consensus CDS
    CCDS57174.1
    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Related
    ENSMUSP00000005365.9, ENSMUST00000005365.15
    Conserved Domains (2) summary
    COG4942
    Location:55 → 129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151 → 220
    Spindle_Spc25; Chromosome segregation protein Spc25

  3. NM_001305800.1 → NP_001292729.1  kinetochore protein Spc25 isoform 1

    See identical proteins and their annotated locations for NP_001292729.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1).
    Source sequence(s)
    AK146727, AL929221, BC033605, CJ086993
    Consensus CDS
    CCDS57174.1
    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Conserved Domains (2) summary
    COG4942
    Location:55 → 129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151 → 220
    Spindle_Spc25; Chromosome segregation protein Spc25

  4. NM_025565.4 → NP_079841.1  kinetochore protein Spc25 isoform 2

    See identical proteins and their annotated locations for NP_079841.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR and lacks an exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform (2), which has a shorter N-terminus compared to isoform 1.
    Source sequence(s)
    AK003408, AL929221, BC033605, CJ086993, CX222514
    Consensus CDS
    CCDS16088.1
    UniProtKB/TrEMBL
    A2AUT4
    Related
    ENSMUSP00000128039.3, ENSMUST00000167875.9
    Conserved Domains (1) summary
    pfam08234
    Location:103 → 172
    Spindle_Spc25; Chromosome segregation protein Spc25

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    69009753..69036583 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036162451.1 → XP_036018344.1  kinetochore protein Spc25 isoform X2

    UniProtKB/TrEMBL
    A2AUT3
    Conserved Domains (1) summary
    COG4942
    Location:55 → 129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]

  2. XM_006500003.4 → XP_006500066.1  kinetochore protein Spc25 isoform X1

    See identical proteins and their annotated locations for XP_006500066.1

    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Conserved Domains (2) summary
    COG4942
    Location:55 → 129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151 → 220
    Spindle_Spc25; Chromosome segregation protein Spc25

  3. XM_036162450.1 → XP_036018343.1  kinetochore protein Spc25 isoform X1

    UniProtKB/Swiss-Prot
    Q3UA16, Q9D021, Q9D1K6
    UniProtKB/TrEMBL
    A2AUT4
    Conserved Domains (2) summary
    COG4942
    Location:55 → 129
    EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]
    pfam08234
    Location:151 → 220
    Spindle_Spc25; Chromosome segregation protein Spc25
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q3UA16.1 GenPept UniProtKB/Swiss-Prot:Q3UA16

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous