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SPAM1 sperm adhesion molecule 1 [ Homo sapiens (human) ]

Gene ID: 6677, updated on 5-Mar-2024

Summary

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Official Symbol
SPAM1provided by HGNC
Official Full Name
sperm adhesion molecule 1provided by HGNC
Primary source
HGNC:HGNC:11217
See related
Ensembl:ENSG00000106304 MIM:600930; AllianceGenome:HGNC:11217
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
Summary
Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
Expression
Restricted expression toward testis (RPKM 5.9) See more
Orthologs
all
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Genomic context

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Location:
7q31.32
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 7 NC_000007.14 (123925241..123971414)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 7 NC_060931.1 (125241959..125288129)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 7 NC_000007.13 (123565295..123611468)

Chromosome 7 - NC_000007.14Genomic Context describing neighboring genes Neighboring gene hyaluronidase 4 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr7:123438019-123438540 Neighboring gene H3K27ac hESC enhancer GRCh37_chr7:123438541-123439062 Neighboring gene RNA, U6 small nuclear 11, pseudogene Neighboring gene hyaluronidase 6, pseudogene Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr7:123534015-123535214 Neighboring gene COX6A1 pseudogene 6 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr7:123634231-123635109 Neighboring gene uncharacterized LOC105375483 Neighboring gene transmembrane protein 229A

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. N-Glycosylation and enzymatic activity of the rHuPH20 expressed in Chinese hamster ovary cells. Liu T, et al. Anal Biochem, 2021 Nov 1. PMID 34520755
  2. Human Hyaluronidase PH20 Potentiates the Antitumor Activities of Mesothelin-Specific CAR-T Cells Against Gastric Cancer. Zhao R, et al. Front Immunol, 2021. PMID 34326835, Free PMC Article
  3. Recombinant human hyaluronidase PH20 does not stimulate an acute inflammatory response and inhibits lipopolysaccharide-induced neutrophil recruitment in the air pouch model of inflammation. Huang Z, et al. J Immunol, 2014 Jun 1. PMID 24778442
  4. SPAM1 (PH-20) protein and mRNA expression in the epididymides of humans and macaques: utilizing laser microdissection/RT-PCR. Evans EA, et al. Reprod Biol Endocrinol, 2003 Aug 6. PMID 12932297, Free PMC Article
  5. The dual functions of GPI-anchored PH-20: hyaluronidase and intracellular signaling. Cherr GN, et al. Matrix Biol, 2001 Dec. PMID 11731269

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Low acrosin activity is associated with decreased Spam1/acrosin expression and GSH deficiency-caused premature acrosome release of human sperm cells.
    Title: Low acrosin activity is associated with decreased Spam1/acrosin expression and GSH deficiency-caused premature acrosome release of human sperm cells.
  2. N-Glycosylation and enzymatic activity of the rHuPH20 expressed in Chinese hamster ovary cells.
    Title: N-Glycosylation and enzymatic activity of the rHuPH20 expressed in Chinese hamster ovary cells.
  3. Human Hyaluronidase PH20 Potentiates the Antitumor Activities of Mesothelin-Specific CAR-T Cells Against Gastric Cancer.
    Title: Human Hyaluronidase PH20 Potentiates the Antitumor Activities of Mesothelin-Specific CAR-T Cells Against Gastric Cancer.
  4. Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice.
    Title: Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice.
  5. results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch.
    Title: Recombinant human hyaluronidase PH20 does not stimulate an acute inflammatory response and inhibits lipopolysaccharide-induced neutrophil recruitment in the air pouch model of inflammation.
  6. HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1.
    Title: Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition.
  7. The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction.
    Title: The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition.
  8. PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination.
    Title: Digestion products of the PH20 hyaluronidase inhibit remyelination.
  9. Observational study of gene-disease association. (HuGE Navigator)
    Title: Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia.
  10. SPAM1 mRNA & protein occur in all 3 regions of epididymis & vas deferens. It has hyaluronidase activity at pH 7.0. The proximal promoter has epididymal transcription factor sites including androgen receptor elements. Its role may be in sperm maturation.
    Title: SPAM1 (PH-20) protein and mRNA expression in the epididymides of humans and macaques: utilizing laser microdissection/RT-PCR.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC26532

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables hyalurononglucosaminidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in binding of sperm to zona pellucida TAS
Traceable Author Statement
more info
 PubMed 
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in cell adhesion IEA
Inferred from Electronic Annotation
more info
  
involved_in fusion of sperm to egg plasma membrane involved in single fertilization IEA
Inferred from Electronic Annotation
more info
  
involved_in hyaluronan catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in acrosomal vesicle IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane TAS
Traceable Author Statement
more info
  
located_in side of membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
hyaluronidase PH-20
Names
PH-20 hyaluronidase
epididymis secretory sperm binding protein Li 96n
hyal-PH20
hyaluronoglucosaminidase PH-20
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
sperm surface protein PH-20
NP_001167515.1
NP_001167516.1
NP_001167517.1
NP_003108.2
NP_694859.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001174044.2 → NP_001167515.1  hyaluronidase PH-20 isoform 2

    See identical proteins and their annotated locations for NP_001167515.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform.
    Source sequence(s)
    AY920278, X84347
    Consensus CDS
    CCDS5791.1
    UniProtKB/Swiss-Prot
    P38567, Q8TC30
    UniProtKB/TrEMBL
    Q5D1J4
    Related
    ENSP00000417934.1, ENST00000460182.5
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 369
    Glyco_hydro_56; Hyaluronidase

  2. NM_001174045.2 → NP_001167516.1  hyaluronidase PH-20 isoform 2

    See identical proteins and their annotated locations for NP_001167516.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform.
    Source sequence(s)
    AK292229, AY920280, X84347
    Consensus CDS
    CCDS5791.1
    UniProtKB/Swiss-Prot
    P38567, Q8TC30
    UniProtKB/TrEMBL
    Q5D1J4
    Related
    ENSP00000402123.1, ENST00000439500.5
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 369
    Glyco_hydro_56; Hyaluronidase

  3. NM_001174046.2 → NP_001167517.1  hyaluronidase PH-20 isoform 2

    See identical proteins and their annotated locations for NP_001167517.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) differs in the 5' UTR and uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform.
    Source sequence(s)
    AK292229, AY920281, X84347
    Consensus CDS
    CCDS5791.1
    UniProtKB/Swiss-Prot
    P38567, Q8TC30
    UniProtKB/TrEMBL
    Q5D1J4
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 369
    Glyco_hydro_56; Hyaluronidase

  4. NM_003117.5 → NP_003108.2  hyaluronidase PH-20 isoform 1

    See identical proteins and their annotated locations for NP_003108.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AC004690, AK292229, AY920279, BC026163
    Consensus CDS
    CCDS5790.1
    UniProtKB/Swiss-Prot
    P38567
    UniProtKB/TrEMBL
    Q5D1J4
    Related
    ENSP00000345849.5, ENST00000340011.9
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 369
    Glyco_hydro_56; Hyaluronidase

  5. NM_153189.3 → NP_694859.1  hyaluronidase PH-20 isoform 2

    See identical proteins and their annotated locations for NP_694859.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter and distinct C-terminus (isoform 2). Variants 2-5 all encode the same isoform.
    Source sequence(s)
    AY920279, X84347
    Consensus CDS
    CCDS5791.1
    UniProtKB/Swiss-Prot
    P38567, Q8TC30
    UniProtKB/TrEMBL
    Q5D1J4
    Related
    ENSP00000508393.1, ENST00000682466.1
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 369
    Glyco_hydro_56; Hyaluronidase

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000007.14 Reference GRCh38.p14 Primary Assembly

    Range
    123925241..123971414
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060931.1 Alternate T2T-CHM13v2.0

    Range
    125241959..125288129
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38567.1 GenPept UniProtKB/Swiss-Prot:P38567

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