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Tysnd1 trypsin domain containing 1 [ Mus musculus (house mouse) ]

Gene ID: 71767, updated on 5-Mar-2024

Summary

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Official Symbol
Tysnd1provided by MGI
Official Full Name
trypsin domain containing 1provided by MGI
Primary source
MGI:MGI:1919017
See related
Ensembl:ENSMUSG00000020087 AllianceGenome:MGI:1919017
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
1300019N10Rik
Summary
This gene encodes a protease that removes the N-terminal peroxisomal targeting signal (PTS2) from proteins produced in the cytosol, thereby facilitating their import into the peroxisome. The encoded protein is also capable of removing the C-terminal peroxisomal targeting signal (PTS1) from proteins in the peroxisomal matrix. The full-length protein undergoes self-cleavage to produce shorter, potentially inactive, peptides. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013]
Expression
Broad expression in adrenal adult (RPKM 220.9), duodenum adult (RPKM 66.0) and 18 other tissues See more
Orthologs
human all
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Genomic context

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Location:
10 B4; 10 32.26 cM
Exon count:
4
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (61531279..61538552)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (61695500..61702773)

Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 1700022H01 gene Neighboring gene STARR-positive B cell enhancer ABC_E9032 Neighboring gene secretion associated Ras related GTPase 1A Neighboring gene STARR-positive B cell enhancer ABC_E1800 Neighboring gene apoptosis-inducing factor, mitochondrion-associated 2 Neighboring gene STARR-seq mESC enhancer starr_26853 Neighboring gene macroH2A.2 histone Neighboring gene STARR-seq mESC enhancer starr_26854 Neighboring gene microRNA 5108

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Sequence-based discovery of the human and rodent peroxisomal proteome. Kurochkin IV, et al. Appl Bioinformatics, 2005. PMID 16128611
  2. Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility. Mizuno Y, et al. PLoS Genet, 2013. PMID 23459139, Free PMC Article
  3. Novel peroxisomal protease Tysnd1 processes PTS1- and PTS2-containing enzymes involved in beta-oxidation of fatty acids. Kurochkin IV, et al. EMBO J, 2007 Feb 7. PMID 17255948, Free PMC Article
  4. Two proteases, trypsin domain-containing 1 (Tysnd1) and peroxisomal lon protease (PsLon), cooperatively regulate fatty acid β-oxidation in peroxisomal matrix. Okumoto K, et al. J Biol Chem, 2011 Dec 30. PMID 22002062, Free PMC Article
  5. A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. Yokoyama S, et al. Dev Cell, 2009 Dec. PMID 20059953, Free PMC Article

See all (18) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Our data established that peroxisomal processing protease Tysnd1 is necessary to mediate the physiological functions of PTS2-containing substrates.
    Title: Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.
  2. characterization of function of human TYSND1, including putative regulation by self-cleavage
    Title: Two proteases, trypsin domain-containing 1 (Tysnd1) and peroxisomal lon protease (PsLon), cooperatively regulate fatty acid β-oxidation in peroxisomal matrix.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables protease binding ISO
Inferred from Sequence Orthology
more info
  
enables serine-type endopeptidase activity EXP
Inferred from Experiment
more info
 PubMed 
enables serine-type endopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables serine-type endopeptidase activity ISO
Inferred from Sequence Orthology
more info
  
enables serine-type peptidase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in protein processing IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein processing ISO
Inferred from Sequence Orthology
more info
  
involved_in proteolysis ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of fatty acid beta-oxidation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of fatty acid beta-oxidation ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in peroxisomal matrix TAS
Traceable Author Statement
more info
  
is_active_in peroxisome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in peroxisome ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
peroxisomal leader peptide-processing protease
Names
trypsin domain-containing protein 1
NP_001259019.1
NP_001259020.1
NP_001259021.1
NP_082188.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001272090.1 → NP_001259019.1  peroxisomal leader peptide-processing protease isoform b

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b) is shorter and has a distinct C-terminus, compared to isoform a.
    Source sequence(s)
    AC153136, AI181257, AK005069
    UniProtKB/Swiss-Prot
    Q9DBA6
    Conserved Domains (2) summary
    pfam13365
    Location:359 → 429
    Trypsin_2; Trypsin-like peptidase domain
    cl21584
    Location:187 → 268
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

  2. NM_001272091.1 → NP_001259020.1  peroxisomal leader peptide-processing protease isoform c

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks an alternate exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (c) is shorter and has a distinct C-terminus, compared to isoform a.
    Source sequence(s)
    AC153136, AK005069
    UniProtKB/Swiss-Prot
    Q9DBA6
    Conserved Domains (1) summary
    cl21584
    Location:187 → 268
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

  3. NM_001272092.1 → NP_001259021.1  peroxisomal leader peptide-processing protease isoform d

    See identical proteins and their annotated locations for NP_001259021.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) lacks an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (d) is shorter than isoform a.
    Source sequence(s)
    AC153136, AK005069
    UniProtKB/Swiss-Prot
    Q9DBA6
    Conserved Domains (2) summary
    pfam13365
    Location:359 → 460
    Trypsin_2; Trypsin-like peptidase domain
    cl21584
    Location:187 → 268
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

  4. NM_027912.1 → NP_082188.1  peroxisomal leader peptide-processing protease isoform a

    See identical proteins and their annotated locations for NP_082188.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
    Source sequence(s)
    AC153136, AK005069, AV148467
    Consensus CDS
    CCDS48575.1
    UniProtKB/Swiss-Prot
    Q0VE90, Q9DBA6
    Related
    ENSMUSP00000020284.5, ENSMUST00000020284.5
    Conserved Domains (3) summary
    pfam00089
    Location:344 → 528
    Trypsin; Trypsin
    pfam13365
    Location:359 → 498
    Trypsin_2; Trypsin-like peptidase domain
    cl21584
    Location:187 → 268
    Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

RNA

  1. NR_073587.1 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) differs in the 5' exon and lacks two alternate internal exons, compared to variant 1. This variant is represented as non-coding because it lacks a large portion of the coding region, including the translational start codon, found in variant 1.
    Source sequence(s)
    AC153136, AK005069, BE847683

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000076.7 Reference GRCm39 C57BL/6J

    Range
    61531279..61538552
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

RNA

  1. XR_003948807.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9DBA6.1 GenPept UniProtKB/Swiss-Prot:Q9DBA6

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