Cybrd1 cytochrome b reductase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cybrd1provided by MGI
Official Full Name: cytochrome b reductase 1provided by MGI
Primary source: MGI:MGI:2654575
See related: Ensembl:ENSMUSG00000027015 AllianceGenome:MGI:2654575
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Dcytb; 2210407P13Rik
Summary: Enables transmembrane ascorbate ferrireductase activity. Acts upstream of or within response to iron ion. Located in brush border membrane. Is expressed in several structures, including central nervous system; foregut; gonad; retina; and trachea. Orthologous to human CYBRD1 (cytochrome b reductase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in stomach adult (RPKM 11.1), limb E14.5 (RPKM 7.2) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 C2; 2 42.38 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (70948398..70973270)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (71118054..71142926)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

It was concluded that Cybrd1 is the primary iron-regulated duodenal ferric reductase in the gut and that it is necessary for optimal iron metabolism.
Title: Duodenal reductase activity and spleen iron stores are reduced and erythropoiesis is abnormal in Dcytb knockout mice exposed to hypoxic conditions.
Increased iron absorption reported in early hypoxia could be accounted for in part by the enhancement of Dcytb expression by Hif-2alpha in the duodenum.
Title: Duodenal cytochrome b (Cybrd 1) and HIF-2α expression during acute hypoxic exposure in mice.
Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter 1 (DMT1) and copper transporter 1, respectively.
Title: Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro.
Iron absorption genes(Dcytb,IREG1,DMT1)were expressed at lower levels in mouse caecum and colon than in the duodenum. They are regulated by body iron requirements.
Title: Expression of iron absorption genes in mouse large intestine.
Dcytb, DMT1, Ireg1 and transferrin receptor 1 have roles in iron transport and hemolysis
Title: Tissue-specific changes in iron metabolism genes in mice following phenylhydrazine-induced haemolysis.
cybrd1 is nonessential in mice
Title: The role of duodenal cytochrome b in intestinal iron absorption remains unclear.
loss of Cybrd1 had little or no impact on body iron stores, even in the setting of iron deficiency; other mechanisms must be available for the reduction of dietary iron
Title: Cybrd1 (duodenal cytochrome b) is not necessary for dietary iron absorption in mice.
Regulatory defects in liver and intestine implicate abnormal hepcidin and Cybrd1 expression in hemochromatosis.
Title: Regulatory defects in liver and intestine implicate abnormal hepcidin and Cybrd1 expression in mouse hemochromatosis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IBA Inferred from Biological aspect of Ancestor more info
enables oxidoreductase activity, acting on metal ions	ISO Inferred from Sequence Orthology more info
enables transmembrane ascorbate ferrireductase activity	IDA Inferred from Direct Assay more info	PubMed
enables transmembrane ascorbate ferrireductase activity	ISO Inferred from Sequence Orthology more info
enables transmembrane monodehydroascorbate reductase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ascorbate homeostasis	ISO Inferred from Sequence Orthology more info
involved_in intracellular iron ion homeostasis	ISO Inferred from Sequence Orthology more info
involved_in multicellular organismal-level iron ion homeostasis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to iron ion	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in brush border membrane	IDA Inferred from Direct Assay more info	PubMed
located_in brush border membrane	ISO Inferred from Sequence Orthology more info
is_active_in lysosomal membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: plasma membrane ascorbate-dependent reductase CYBRD1

Names: duodenal cytochrome b

NP_082869.2

EC 7.2.1.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_028593.2 → NP_082869.2 plasma membrane ascorbate-dependent reductase CYBRD1

See identical proteins and their annotated locations for NP_082869.2

Status: VALIDATED

Source sequence(s)

AK029112, AL929228

Consensus CDS

CCDS50603.1

UniProtKB/Swiss-Prot

A2AUU8, Q925G2, Q9D7U1

Related

ENSMUSP00000028403.3, ENSMUST00000028403.3

Conserved Domains (1) summary

cl23723
Location:36 → 187

Cytochrome_b_N; Cytochrome b (N-terminus)/b6/petB: Cytochrome b is a subunit of cytochrome bc1, an 11-subunit mitochondrial respiratory enzyme. Cytochrome b spans the mitochondrial membrane with 8 transmembrane helices (A-H) in eukaryotes. In plants and cyanobacteria, ...