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Rnf145 ring finger protein 145 [ Mus musculus (house mouse) ]

Gene ID: 74315, updated on 5-Mar-2024

Summary

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Official Symbol
Rnf145provided by MGI
Official Full Name
ring finger protein 145provided by MGI
Primary source
MGI:MGI:1921565
See related
Ensembl:ENSMUSG00000019189 AllianceGenome:MGI:1921565
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
TMRF1; 3732413I11Rik
Summary
Predicted to enable ubiquitin protein ligase activity. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in endoplasmic reticulum. Is expressed in early conceptus and genitourinary system. Orthologous to human RNF145 (ring finger protein 145). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in thymus adult (RPKM 28.4), CNS E11.5 (RPKM 19.9) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
11 B1.1; 11 25.94 cM
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (44409486..44456347)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (44518793..44565520)

Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E5216 Neighboring gene STARR-positive B cell enhancer mm9_chr11:44292046-44292346 Neighboring gene STARR-positive B cell enhancer ABC_E3941 Neighboring gene STARR-positive B cell enhancer ABC_E6969 Neighboring gene ubiquitin-like domain containing CTD phosphatase 1 Neighboring gene adenosine kinase pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E8391 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:44358832-44358985 Neighboring gene predicted gene, 51891 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:44391554-44391757 Neighboring gene RIKEN cDNA 4930597A21 gene Neighboring gene STARR-positive B cell enhancer mm9_chr11:44399542-44399843 Neighboring gene STARR-positive B cell enhancer ABC_E6970 Neighboring gene predicted gene, 39815 Neighboring gene STARR-positive B cell enhancer mm9_chr11:44449840-44450140 Neighboring gene STARR-positive B cell enhancer ABC_E8392 Neighboring gene STARR-positive B cell enhancer ABC_E5217 Neighboring gene early B cell factor 1 Neighboring gene STARR-seq mESC enhancer starr_29281 Neighboring gene STARR-positive B cell enhancer ABC_E10669 Neighboring gene STARR-seq mESC enhancer starr_29286 Neighboring gene STARR-seq mESC enhancer starr_29287 Neighboring gene STARR-positive B cell enhancer ABC_E1821 Neighboring gene STARR-seq mESC enhancer starr_29288 Neighboring gene STARR-seq mESC enhancer starr_29289 Neighboring gene STARR-seq mESC enhancer starr_29290 Neighboring gene STARR-positive B cell enhancer mm9_chr11:44713071-44713372 Neighboring gene STARR-seq mESC enhancer starr_29291 Neighboring gene STARR-positive B cell enhancer mm9_chr11:44763908-44764208 Neighboring gene STARR-seq mESC enhancer starr_29292

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Inhibition of cholesterol biosynthesis through RNF145-dependent ubiquitination of SCAP. Zhang L, et al. Elife, 2017 Oct 25. PMID 29068315, Free PMC Article
  2. The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. Menzies SA, et al. Elife, 2018 Dec 13. PMID 30543180, Free PMC Article
  3. Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene. Cook EC, et al. PLoS One, 2017. PMID 28231341, Free PMC Article
  4. Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst. Graham DB, et al. Nat Commun, 2015 Jul 21. PMID 26194095, Free PMC Article
  5. Identification of candidate maternal-effect genes through comparison of multiple microarray data sets. Mager J, et al. Mamm Genome, 2006 Sep. PMID 16964442

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. n the absence of both RNF145 and gp78, Hrd1, a third UBE2G2-dependent E3 ligase, partially regulates HMGCR activity. Our findings reveal a critical role for the sterol-responsive RNF145 in HMGCR regulation and elucidate the complexity of sterol-accelerated HMGCR degradation
    Title: The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.
  2. Mechanistic studies show that RNF145 triggers ubiquitination of SCAP on lysine residues within a cytoplasmic loop essential for COPII binding, potentially inhibiting its transport to Golgi and subsequent processing of SREBP-2.
    Title: Inhibition of cholesterol biosynthesis through RNF145-dependent ubiquitination of SCAP.
  3. we report the identification of RNF145 as an ER-resident E3 ubiquitin ligase that is transcriptionally controlled by LXR.
    Title: Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene.
  4. implicated in proteostasis of Nox2 complex
    Title: Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Endonuclease-mediated (2) 

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in endomembrane system IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
RING finger protein 145
NP_001160025.1
NP_001350095.1
NP_083138.2
XP_006534420.1
XP_006534421.1
XP_030102252.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001166553.1 → NP_001160025.1  RING finger protein 145 isoform 2

    See identical proteins and their annotated locations for NP_001160025.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and 3' coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.
    Source sequence(s)
    AK139310, AL603913
    Consensus CDS
    CCDS48775.1
    UniProtKB/TrEMBL
    Q8BU61
    Related
    ENSMUSP00000098881.3, ENSMUST00000101327.3
    Conserved Domains (1) summary
    pfam13705
    Location:8 → 265
    TRC8_N; TRC8 N-terminal domain

  2. NM_001363166.1 → NP_001350095.1  RING finger protein 145 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AL603913
    Consensus CDS
    CCDS24565.1
    UniProtKB/Swiss-Prot
    Q5SWK7, Q8BXX5, Q9CXG1
    Conserved Domains (3) summary
    COG5243
    Location:520 → 627
    HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
    cd16684
    Location:533 → 575
    RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
    pfam13705
    Location:8 → 506
    TRC8_N; TRC8 N-terminal domain

  3. NM_028862.4 → NP_083138.2  RING finger protein 145 isoform 1

    See identical proteins and their annotated locations for NP_083138.2

    Status: VALIDATED

    Source sequence(s)
    AK014408, AK043002, AL603913, BB618481, CK624836
    Consensus CDS
    CCDS24565.1
    UniProtKB/Swiss-Prot
    Q5SWK7, Q8BXX5, Q9CXG1
    Related
    ENSMUSP00000019333.4, ENSMUST00000019333.10
    Conserved Domains (3) summary
    COG5243
    Location:520 → 627
    HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
    cd16684
    Location:533 → 575
    RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
    pfam13705
    Location:8 → 506
    TRC8_N; TRC8 N-terminal domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000077.7 Reference GRCm39 C57BL/6J

    Range
    44409486..44456347
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006534358.4 → XP_006534421.1  RING finger protein 145 isoform X1

    See identical proteins and their annotated locations for XP_006534421.1

    UniProtKB/Swiss-Prot
    Q5SWK7, Q8BXX5, Q9CXG1
    Conserved Domains (3) summary
    COG5243
    Location:520 → 627
    HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
    cd16684
    Location:533 → 575
    RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
    pfam13705
    Location:8 → 506
    TRC8_N; TRC8 N-terminal domain

  2. XM_006534357.3 → XP_006534420.1  RING finger protein 145 isoform X1

    See identical proteins and their annotated locations for XP_006534420.1

    UniProtKB/Swiss-Prot
    Q5SWK7, Q8BXX5, Q9CXG1
    Conserved Domains (3) summary
    COG5243
    Location:520 → 627
    HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
    cd16684
    Location:533 → 575
    RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
    pfam13705
    Location:8 → 506
    TRC8_N; TRC8 N-terminal domain

  3. XM_030246392.1 → XP_030102252.1  RING finger protein 145 isoform X2

    Conserved Domains (3) summary
    COG5243
    Location:408 → 515
    HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
    cd16684
    Location:421 → 463
    RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
    pfam13705
    Location:1 → 394
    TRC8_N; TRC8 N-terminal domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q5SWK7.1 GenPept UniProtKB/Swiss-Prot:Q5SWK7

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