Neto2 neuropilin (NRP) and tolloid (TLL)-like 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Neto2provided by MGI
Official Full Name: neuropilin (NRP) and tolloid (TLL)-like 2provided by MGI
Primary source: MGI:MGI:1921763
See related: Ensembl:ENSMUSG00000036902 AllianceGenome:MGI:1921763
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: BTCL2; 5530601C23Rik
Summary: Enables ionotropic glutamate receptor binding activity. Predicted to be involved in regulation of kainate selective glutamate receptor activity. Located in postsynaptic density. Is expressed in central nervous system and retina. Orthologous to human NETO2 (neuropilin and tolloid like 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in CNS E18 (RPKM 19.7), whole brain E14.5 (RPKM 13.2) and 7 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C3; 8 41.61 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (86363347..86428011, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (85636718..85700952, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NETO2 promotes esophageal cancer progression by inducing proliferation and metastasis via PI3K/AKT and ERK pathway.
Title: NETO2 promotes esophageal cancer progression by inducing proliferation and metastasis via PI3K/AKT and ERK pathway.
Kainate Receptor Auxiliary Subunit NETO2-Related Cued Fear Conditioning Impairments Associate with Defects in Amygdala Development and Excitability.
Title: Kainate Receptor Auxiliary Subunit NETO2-Related Cued Fear Conditioning Impairments Associate with Defects in Amygdala Development and Excitability.
in cued fear conditioning, Neto2(-/-), but not Neto1(-/-) mice, showed higher fear expression and delayed extinction compared to wild type mice. We established, by in situ hybridization, that Neto2 was expressed in both excitatory and inhibitory neurons throughout the fear circuit including the medial prefrontal cortex, amygdala, and hippocampus.
Title: Kainate receptor auxiliary subunit NETO2 is required for normal fear expression and extinction.
KAR-mediated excitation of interneurons is contingent upon Neto1 because kainate yields comparable effects in Neto2 knockouts and wild-types but fails to excite interneurons or recruit inhibition in Neto1 knockouts.
Title: Neto Auxiliary Subunits Regulate Interneuron Somatodendritic and Presynaptic Kainate Receptors to Control Network Inhibition.
Pain-transducing peripheral sensory neurons of the dorsal root ganglia (DRG) express kainate receptors (KARs), a subfamily of glutamate receptors that modulate neurite outgrowth and regulate glutamate release at the DRG-dorsal horn synapse.
Title: Neto2 Assembles with Kainate Receptors in DRG Neurons during Development and Modulates Neurite Outgrowth in Adult Sensory Neurons.
These results establish critical roles for Neto auxiliary subunits controlling Kainate receptor properties and synaptic incorporation.
Title: Neto auxiliary proteins control both the trafficking and biophysical properties of the kainate receptor GluK1.
Neto2 interacts with the scaffolding protein GRIP and regulates synaptic abundance of kainate receptors.
Title: Neto2 interacts with the scaffolding protein GRIP and regulates synaptic abundance of kainate receptors.
Loss of the Neto2:KCC2 interaction reduced KCC2-mediated Cl(-) extrusion, resulting in decreased synaptic inhibition in hippocampal neurons.
Title: Neto2 is a KCC2 interacting protein required for neuronal Cl- regulation in hippocampal neurons.
Btcl2 mRNA was observed restrictively in brain throughout embryonic and postnatal stages; expressed in the pontine nucleus and subplate, which are required for establishing the neuronal circuit formation.
Title: Expression of Btcl2, a novel member of Btcl gene family, during development of the central nervous system.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2) 1 citation
Endonuclease-mediated (5)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D8Mit79 (e-PCR), detects polymorphism
- UniSTS:131625

AI854825 (e-PCR)
- UniSTS:165592

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ionotropic glutamate receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in neurotransmitter receptor localization to postsynaptic specialization membrane	ISO Inferred from Sequence Orthology more info
NOT acts_upstream_of_or_within positive regulation of excitatory postsynaptic potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neurotransmitter receptor localization to postsynaptic specialization membrane	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IEP Inferred from Expression Pattern more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density membrane	IEP Inferred from Expression Pattern more info	PubMed
located_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: neuropilin and tolloid-like protein 2

Names: brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081324.2 → NP_001074793.1 neuropilin and tolloid-like protein 2 isoform 3

See identical proteins and their annotated locations for NP_001074793.1

Status: VALIDATED

Source sequence(s)

AW544441, AY138991, BC068168

Consensus CDS

CCDS40423.1

UniProtKB/Swiss-Prot

Q8BNJ6

Related

ENSMUSP00000105308.4, ENSMUST00000109686.4

Conserved Domains (2) summary

cd00041
Location:80 → 193

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00112
Location:325 → 359

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
NM_001357287.1 → NP_001344216.1 neuropilin and tolloid-like protein 2 isoform 1 precursor

Status: VALIDATED

Source sequence(s)

AK083512, AW544441, BC172104

Consensus CDS

CCDS90433.1

UniProtKB/TrEMBL

B2RXQ6

Related

ENSMUSP00000148172.2, ENSMUST00000209479.2

Conserved Domains (2) summary

cd00041
Location:45 → 158

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00112
Location:290 → 324

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
NM_001357288.1 → NP_001344217.1 neuropilin and tolloid-like protein 2 isoform 2 precursor

Status: VALIDATED

Source sequence(s)

AC122409, AK083512, AW544441, BC068168

Consensus CDS

CCDS90432.1

UniProtKB/Swiss-Prot

B2RX93, Q5VM49, Q8BNJ6, Q8C4Q8

Related

ENSMUSP00000150062.2, ENSMUST00000216286.2

Conserved Domains (2) summary

cd00041
Location:45 → 158

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00112
Location:297 → 331

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...

RNA

NR_151665.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC122409, AW544441, BC068168

Related

ENSMUST00000209259.2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

86363347..86428011 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036154338.1 → XP_036010231.1 neuropilin and tolloid-like protein 2 isoform X4

Conserved Domains (2) summary

cd00041
Location:45 → 158

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00112
Location:282 → 316

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
XM_006531437.5 → XP_006531500.1 neuropilin and tolloid-like protein 2 isoform X2

See identical proteins and their annotated locations for XP_006531500.1

UniProtKB/Swiss-Prot

Q8BNJ6

Conserved Domains (2) summary

cd00041
Location:80 → 193

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00112
Location:325 → 359

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
XM_030243818.2 → XP_030099678.1 neuropilin and tolloid-like protein 2 isoform X1

Conserved Domains (2) summary

cd00041
Location:80 → 193

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00112
Location:332 → 366

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
XM_036154337.1 → XP_036010230.1 neuropilin and tolloid-like protein 2 isoform X3

Conserved Domains (2) summary

cd00041
Location:80 → 193

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00112
Location:317 → 351

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
XM_036154339.1 → XP_036010232.1 neuropilin and tolloid-like protein 2 isoform X5

Conserved Domains (3) summary

cd00041
Location:75 → 161

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00112
Location:167 → 201

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...

cl00049
Location:2 → 35

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.