Nell1 neural EGFL like 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nell1provided by RGD
Official Full Name: neural EGFL like 1provided by RGD
Primary source: RGD:620998
See related: Ensembl:ENSRNOG00000015675 AllianceGenome:RGD:620998
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables heparin binding activity; identical protein binding activity; and protein kinase C binding activity. Involved in positive regulation of apoptotic process; positive regulation of ossification; and regulation of osteoblast differentiation. Located in cytoplasm and extracellular space. Used to study craniosynostosis. Orthologous to human NELL1 (neural EGFL like 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 158.3), Lung (RPKM 60.4) and 1 other tissue See more
Orthologs: human mouse all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 1q22

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (108845462..109709858)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (99709305..100573872)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (105348577..106218970)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The expression levels of neural markers were increased in rat pulp and in cultured human dental pulp stem cells stimulated with Nell-1 protein. This is the first study to demonstrate the effect of Nell-1 on dental pulp cells neural-like differentiation.
Title: Nell-1 promotes the neural-like differentiation of dental pulp cells.
These findings suggested that combined use of NELL-1 and BMP2 could positively regulate pulp repair.
Title: Effects of Nel-like molecule-1 and bone morphogenetic protein 2 combination on rat pulp repair.
establish the feasibility of combining NELL-1 with BMP2 to improve clinical bone regeneration and provide mechanistic insight into canonical Wnt pathway activity during NELL-1 and BMP2 osteogenesis
Title: Novel Wnt Regulator NEL-Like Molecule-1 Antagonizes Adipogenesis and Augments Osteogenesis Induced by Bone Morphogenetic Protein 2.
NELL-1570 has the potential to be used for cell-based or hormone-based therapy of bone regeneration.
Title: Proliferation and osteogenic differentiation of mesenchymal stem cells induced by a short isoform of NELL-1.
Thus, NELL-1 effectively enhances in situ osteogenesis in the bone marrow, making it potentially useful in the prevention and treatment of osteoporotic fractures.
Title: NELL-1 injection maintains long-bone quantity and quality in an ovariectomy-induced osteoporotic senile rat model.
Nell-1 can significantly improve bone regeneration in a femoral segmental defect in a dose-dependent manner and is a potent osteospecific growth factor.
Title: Nell-1 enhances bone regeneration in a rat critical-sized femoral segmental defect model.
Data show that Nell-1 overexpression induced calvarial overgrowth resulting in premature suture closure
Title: Craniosynostosis in transgenic mice overexpressing Nell-1.
Nell1 accelerates chondrocyte hypertrophy and endochondral bone formation.
Title: Nell-1 induced bone formation within the distracted intermaxillary suture.
Nell-1 overexpression disrupts these pathways resulting in craniofacial anomalies such as premature suture closure.
Title: Overexpression of Nell-1, a craniosynostosis-associated gene, induces apoptosis in osteoblasts during craniofacial development.

Submit: New GeneRIF Correction

General gene information

Go to the top of the page Help

Markers

d1rat214 (e-PCR)
- UniSTS:120324

d1rat222 (e-PCR)
- UniSTS:120327

RH138223 (e-PCR)
- UniSTS:220310

D1RAT30 (e-PCR)
- UniSTS:121496

AU027942 (e-PCR)
- UniSTS:112813

AU048880 (e-PCR)
- UniSTS:145330

AU026339 (e-PCR)
- UniSTS:114377

D1Rat206 (e-PCR), detects polymorphism
- UniSTS:227060

AU047621 (e-PCR)
- UniSTS:110327

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables heparin binding	IBA Inferred from Biological aspect of Ancestor more info
enables heparin binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase C binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase C binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of osteoblast proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of osteoblast proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of bone mineralization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of bone mineralization	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of ossification	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of ossification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of osteoblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of osteoblast differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in regulation of gene expression	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of osteoblast differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of osteoblast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear envelope	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	ISS Inferred from Sequence or Structural Similarity more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISS Inferred from Sequence or Structural Similarity more info

General protein information

Go to the top of the page Help

Preferred Names: protein kinase C-binding protein NELL1

Names: NEL-like 1; NEL-like protein 1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031069.3 → NP_112331.2 protein kinase C-binding protein NELL1 precursor

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

A6JBG8, D4A284, Q62919

Related

ENSRNOP00000052883.4, ENSRNOT00000056030.5

Conserved Domains (6) summary

smart00179
Location:549 → 586

EGF_CA; Calcium-binding EGF-like domain

smart00210
Location:29 → 213

TSPN; Thrombospondin N-terminal -like domains

pfam07645
Location:596 → 627

EGF_CA; Calcium-binding EGF domain

cl17735
Location:273 → 331

VWC; von Willebrand factor type C domain

pfam12947
Location:480 → 515

EGF_3; EGF domain

NF033759
Location:386 → 556

exchanger_TraA; outer membrane exchange protein TraA

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

108845462..109709858

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063275221.1 → XP_063131291.1 protein kinase C-binding protein NELL1 isoform X3
XM_063275215.1 → XP_063131285.1 protein kinase C-binding protein NELL1 isoform X2
XM_017589771.3 → XP_017445260.1 protein kinase C-binding protein NELL1 isoform X1

UniProtKB/TrEMBL

A0A8I6A059

Related

ENSRNOP00000083529.1
XM_039092192.2 → XP_038948120.1 protein kinase C-binding protein NELL1 isoform X4

UniProtKB/TrEMBL

A6JBG9

Conserved Domains (3) summary

smart00179
Location:434 → 466

EGF_CA; Calcium-binding EGF-like domain

smart00210
Location:29 → 213

TSPN; Thrombospondin N-terminal -like domains

pfam00093
Location:273 → 331

VWC; von Willebrand factor type C domain
XM_039092195.2 → XP_038948123.1 protein kinase C-binding protein NELL1 isoform X5

UniProtKB/TrEMBL

A6JBG9

Conserved Domains (2) summary

smart00210
Location:29 → 213

TSPN; Thrombospondin N-terminal -like domains

pfam00093
Location:273 → 331

VWC; von Willebrand factor type C domain
XM_039092200.2 → XP_038948128.1 protein kinase C-binding protein NELL1 isoform X6

UniProtKB/TrEMBL

A6JBG9

Conserved Domains (2) summary

smart00210
Location:29 → 213

TSPN; Thrombospondin N-terminal -like domains

pfam00093
Location:273 → 331

VWC; von Willebrand factor type C domain