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HSI2 B3 domain-containing transcription repressor VAL1 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 817597, updated on 7-Sep-2023

Summary

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Gene symbol
HSI2
Gene description
B3 domain-containing transcription repressor VAL1
Primary source
Araport:AT2G30470
Locus tag
AT2G30470
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
high-level expression of sugar-inducible gene 2; T6B20.17; T6B20_17; VAL1; VP1/ABI3-LIKE 1
Summary
HSI2 is a member of a novel family of B3 domain proteins with a sequence similar to the ERF-associated amphiphilic repression (EAR) motif. It functions as an active repressor of the Spo minimal promoter (derived from a gene for sweet potato sporamin A1) through the EAR motif. It contains a plant-specific B3 DNA-binding domain. The Arabidopsis genome contains 42 genes with B3 domains which could be classified into three families that are represented by ABI3, ARF1 and RAV1. HSI2 belongs to the ABI3 family. It is expressed at similar levels in all organs. Treatment with 6% sucrose showed a slight increase in transcript levels after 24 h. No changes were observed after treatment with 50##M ABA. It is localized in the nucleus via a nuclear localization sequence located in the fourth conserved region of the C-terminal B3 domain.
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Genomic context

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See HSI2 in Genome Data Viewer
Location:
chromosome: 2
Exon count:
12
Sequence:
Chromosome: 2; NC_003071.7 (12980428..12985277, complement)

Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene tRNA-Gly Neighboring gene Nucleotide/sugar transporter family protein Neighboring gene uncharacterized protein Neighboring gene cinnamate-4-hydroxylase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. EAR domain-containing transcription factors trigger PRC2-mediated chromatin marking in Arabidopsis. Baile F, et al. Plant Cell, 2021 Aug 31. PMID 34003929, Free PMC Article
  2. Structural basis of DNA target recognition by the B3 domain of Arabidopsis epigenome reader VAL1. Sasnauskas G, et al. Nucleic Acids Res, 2018 May 4. PMID 29660015, Free PMC Article
  3. Arabidopsis transcriptional repressor VAL1 triggers Polycomb silencing at FLC during vernalization. Qüesta JI, et al. Science, 2016 Jul 29. PMID 27471304
  4. HSI2/VAL1 PHD-like domain promotes H3K27 trimethylation to repress the expression of seed maturation genes and complex transgenes in Arabidopsis seedlings. Veerappan V, et al. BMC Plant Biol, 2014 Nov 1. PMID 25367506, Free PMC Article
  5. High-level expression of sugar inducible gene2 (HSI2) is a negative regulator of drought stress tolerance in Arabidopsis. Sharma N, et al. BMC Plant Biol, 2013 Oct 29. PMID 24168327, Free PMC Article

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Brassinosteroids repress the seed maturation program during the seed-to-seedling transition.
    Title: Brassinosteroids repress the seed maturation program during the seed-to-seedling transition.
  2. EAR domain-containing transcription factors trigger PRC2-mediated chromatin marking in Arabidopsis.
    Title: EAR domain-containing transcription factors trigger PRC2-mediated chromatin marking in Arabidopsis.
  3. VAL genes regulate vegetative phase change via miR156-dependent and independent mechanisms.
    Title: VAL genes regulate vegetative phase change via miR156-dependent and independent mechanisms.
  4. The transcriptional repressors VAL1 and VAL2 recruit PRC2 for genome-wide Polycomb silencing in Arabidopsis.
    Title: The transcriptional repressors VAL1 and VAL2 recruit PRC2 for genome-wide Polycomb silencing in Arabidopsis.
  5. The B3-Domain Transcription Factor VAL1 Regulates the Floral Transition by Repressing FLOWERING LOCUS T.
    Title: The B3-Domain Transcription Factor VAL1 Regulates the Floral Transition by Repressing FLOWERING LOCUS T.
  6. Structural basis for the Sph/RY sequence recognition by VAL1/2 transcriptional repressor.
    Title: Structural basis of DNA target recognition by the B3 domain of Arabidopsis epigenome reader VAL1.
  7. VAL1 crystal structure provides insight into the important implication of VAL1-B3 domain in flowering process.
    Title: Structural insight into the role of VAL1 B3 domain for targeting to FLC locus in Arabidopsis thaliana.
  8. The results suggest that HSI2 recruits MED13 and HDA6 to suppress directly a subset of seed maturation genes post-germination.
    Title: HSI2 Repressor Recruits MED13 and HDA6 to Down-Regulate Seed Maturation Gene Expression Directly During Arabidopsis Early Seedling Growth.
  9. LEC1, LEC2 , and FUSCA3 transcripts are candidate targets of VAL1, acting through epigenetic and/or transcriptional repression.
    Title: Potential targets of VIVIPAROUS1/ABI3-LIKE1 (VAL1) repression in developing Arabidopsis thaliana embryos.
  10. VAL1 localizes to the nucleation region in vivo, promoting histone deacetylation and FLOWERING LOCUS C transcriptional silencing, and interacts with components of the conserved apoptosis- and splicing-associated protein complex.
    Title: Arabidopsis transcriptional repressor VAL1 triggers Polycomb silencing at FLC during vernalization.
Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

NM_179815.3

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables DNA-binding transcription factor activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within negative regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within positive regulation of seed germination IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within regulation of seed maturation IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within response to abscisic acid TAS
Traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to sucrose TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in mitochondrion HDA PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISM
Inferred from Sequence Model
more info
  

General protein information

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Preferred Names
B3 domain-containing transcription repressor VAL1
NP_850146.1
  • high-level expression of sugar-inducible gene 2 (HSI2); CONTAINS InterPro DOMAIN/s: Transcriptional factor B3 (InterPro:IPR003340), Zinc finger, CW-type (InterPro:IPR011124); BEST Arabidopsis thaliana protein match is: HSI2-like 1 (TAIR:AT4G32010.1); Has 1263 Blast hits to 1242 proteins in 97 species: Archae - 0; Bacteria - 5; Metazoa - 31; Fungi - 0; Plants - 1168; Viruses - 0; Other Eukaryotes - 59 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003071.7 Reference assembly

    Range
    12980428..12985277 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_179815.3 → NP_850146.1  B3 domain-containing transcription repressor VAL1 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_850146.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    O04346, Q8W4L5
    UniProtKB/TrEMBL
    A0A178VNU8, A0A5S9X2U9
    Conserved Domains (2) summary
    cd10017
    Location:293 → 390
    B3_DNA; Plant-specific B3-DNA binding domain
    pfam07496
    Location:544 → 585
    zf-CW; CW-type Zinc Finger
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8W4L5.1

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