CYP94B3 cytochrome P450, family 94, subfamily B, polypeptide 3 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Gene symbol: CYP94B3
Gene description: cytochrome P450, family 94, subfamily B, polypeptide 3
Primary source: Araport:AT3G48520
Locus tag: AT3G48520
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: cytochrome P450; family 94; polypeptide 3; subfamily B
Summary: CYP94B3 is a jasmonoyl-isoleucine-12-hydroxylase that catalyzes the formation of 12-OH-JA-Ile from JA-Ile. By reducing the levels of this the biologically active phytohormone, CYP94B3 attenuates the jasmonic acid signaling cascade. CYP94B3 transcript levels rise in response to wounding.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See CYP94B3 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 1

Sequence:: Chromosome: 3; NC_003074.8 (17974853..17976774, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

WRKY9 transcription factor regulates cytochrome P450 genes CYP94B3 and CYP86B1, leading to increased root suberin and salt tolerance in Arabidopsis.
Title: WRKY9 transcription factor regulates cytochrome P450 genes CYP94B3 and CYP86B1, leading to increased root suberin and salt tolerance in Arabidopsis.
we created the double and triple knock-out mutants of three CYP94 genes, CYP94B1, CYP94B3, and CYP94C1. The mutations blocked the oxidation steps and caused JA-Ile to accumulate 3-4-fold the WT levels in the wounded leaves.
Title: Mutations in jasmonoyl-L-isoleucine-12-hydroxylases suppress multiple JA-dependent wound responses in Arabidopsis thaliana.
CYP94B3 and CYP94C1 are responsible for the production of oxidized derivatives of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine in wounded plants.
Title: Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates.
These results highlight the dual function of CYP94B3/C1 in antimicrobial defence.
Title: CYP94-mediated jasmonoyl-isoleucine hormone oxidation shapes jasmonate profiles and attenuates defence responses to Botrytis cinerea infection.
It was found that CYP94B3 also catalyzes the hydroxylation of jasmonoyl-L-valine and jasmonoyl-L-phenylalanine, and that these hydroxyl compounds accumulated after wounding and possessed lower activity than non-hydroxylated compounds.(cytohrome P-450 94B3)
Title: CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-β-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite.
These results identify CYP94B3/C1-mediated oxidation as a major catabolic route for turning over the JA-Ile hormone.
Title: Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone Jasmonoyl-isoleucine for catabolic turnover.
CYP94B3 plays a major regulatory role in controlling the level of jasmonyl-L-isoleucine in plants.
Title: Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

NM_114710.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables heme binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables jasmonoyl-isoleucine-12-hydroxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within anther development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to insect	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within fruit development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within jasmonic acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pollen development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to wounding	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within stigma development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	ISM Inferred from Sequence Model more info

General protein information

Go to the top of the page Help

Preferred Names: cytochrome P450, family 94, subfamily B, polypeptide 3

NP_190421.1

''cytochrome P450, family 94, subfamily B, polypeptide 3'' (CYP94B3); FUNCTIONS IN: electron carrier activity, monooxygenase activity, iron ion binding, oxygen binding, heme binding; INVOLVED IN: oxidation reduction; LOCATED IN: endomembrane system; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 94, subfamily B, polypeptide 1 (TAIR:AT5G63450.1); Has 27655 Blast hits to 27536 proteins in 1435 species: Archae - 44; Bacteria - 2616; Metazoa - 10459; Fungi - 5693; Plants - 7745; Viruses - 3; Other Eukaryotes - 1095 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

17974853..17976774 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_114710.3 → NP_190421.1 cytochrome P450, family 94, subfamily B, polypeptide 3 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_190421.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9SMP5

UniProtKB/TrEMBL

A0A178V8H3, A0A5S9XJ92

Conserved Domains (1) summary

cl12078
Location:46 → 501

p450; Cytochrome P450