NIFK nucleolar protein interacting with the FHA domain of MKI67 [Homo sapiens (human)] - Gene

Summary

Official Symbol: NIFKprovided by HGNC
Official Full Name: nucleolar protein interacting with the FHA domain of MKI67provided by HGNC
Primary source: HGNC:HGNC:17838
See related: Ensembl:ENSG00000155438 MIM:611970; AllianceGenome:HGNC:17838
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: Nopp34; MKI67IP
Summary: This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009]
Expression: Ubiquitous expression in lymph node (RPKM 19.8), appendix (RPKM 17.9) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 2q14.3

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	2	NC_000002.12 (121726945..121736875, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	2	NC_060926.1 (122162220..122172150, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	2	NC_000002.11 (122484521..122494451, complement)

Chromosome 2 - NC_000002.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NIFK as a potential prognostic biomarker in colorectal cancer correlating with immune infiltrates.
Title: NIFK as a potential prognostic biomarker in colorectal cancer correlating with immune infiltrates.
NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation.
Title: The nucleolar protein NIFK promotes cancer progression via CK1α/β-catenin in metastasis and Ki-67-dependent cell proliferation.
The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
Title: The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
Potential encounter complexes of the Ki67FHA receptor and hNIFK peptide are misregistered states of the beta-sheet.
Title: Direct observations of shifts in the β-sheet register of a protein-peptide complex using explicit solvent simulations.
nmr analysis of the solution structure of the FHA domain of human Ki67 and mapping of the binding surface for NIFK binding
Title: Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Pr55(Gag)	gag	Tandem affinity purification and mass spectrometry analysis identify Nopp34 associated HIV-1 Gag in 293T cells	PubMed
retropepsin	gag-pol	Positional proteomics analysis identifies the cleavage of human MKI67 (FHA domain) interacting nucleolar phosphoprotein (MKI67IP) at amino acid residues 188-189 by the HIV-1 protease	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

A004C36 (e-PCR)
- UniSTS:25050

RH12564 (e-PCR)
- UniSTS:76540

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables RNA binding	HDA more info	PubMed
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein-containing complex assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in rRNA metabolic process	NAS Non-traceable Author Statement more info	PubMed
involved_in rRNA transcription	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IDA Inferred from Direct Assay more info
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: MKI67 FHA domain-interacting nucleolar phosphoprotein

Names: hNIFK; nucleolar phosphoprotein Nopp34; nucleolar protein interacting with the FHA domain of pKi-67

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_032390.5 → NP_115766.3 MKI67 FHA domain-interacting nucleolar phosphoprotein

See identical proteins and their annotated locations for NP_115766.3

Status: REVIEWED

Source sequence(s)

BC022990, BC024238, BE671589

Consensus CDS

CCDS2135.1

UniProtKB/Swiss-Prot

A8K788, Q8TB66, Q96ED4, Q9BYG3

Related

ENSP00000285814.4, ENST00000285814.9

Conserved Domains (3) summary

cd12307
Location:46 → 119

RRM_NIFK_like; RNA recognition motif in nucleolar protein interacting with the FHA domain of pKI-67 (NIFK) and similar proteins

pfam12196
Location:227 → 266

hNIFK_binding; FHA Ki67 binding domain of hNIFK

cl25887
Location:2 → 151

RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]