ALG7 UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ALG7
Gene description: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Primary source: SGD:S000000447
Locus tag: YBR243C
See related: AllianceGenome:SGD:S000000447
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TUR1
Summary: Enables UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity. Involved in aerobic respiration and protein N-linked glycosylation. Located in endoplasmic reticulum. Part of UDP-N-acetylglucosamine transferase complex. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation Ij and congenital myasthenic syndrome 13. Orthologous to human DPAGT1 (dolichyl-phosphate N-acetylglucosaminephosphotransferase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (705447..706793, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The N- and C-termini of Alg14 coordinate recruitment of catalytic Alg7 and Alg13 to the endoplasmic reticulum membrane for initiating lipid-linked oligosaccharide biosynthesis.
Title: Alg14 organizes the formation of a multiglycosyltransferase complex involved in initiation of lipid-linked oligosaccharide biosynthesis.
Data show that a conditional lethal mutation in the alg7 gene was successfully complemented with Mv1751 gene from Methanococcus voltae.
Title: Identification of the archaeal alg7 gene homolog (encoding N-acetylglucosamine-1-phosphate transferase) of the N-linked glycosylation system by cross-domain complementation in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity	IEA Inferred from Electronic Annotation more info
enables UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in aerobic respiration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dolichol-linked oligosaccharide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in protein N-linked glycosylation	IEA Inferred from Electronic Annotation more info
involved_in protein N-linked glycosylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein glycosylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of UDP-N-acetylglucosamine transferase complex	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

NP_009802.3

EC 2.7.8.15

UDP-N-acetyl-glucosamine-1-P transferase; transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin; human homolog DPAGT1 can complement yeast ALG7 mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

705447..706793 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178591.3 → NP_009802.3 TPA: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009802.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQN9, P07286

UniProtKB/TrEMBL

A0A6A5Q258, A6ZLI2, B3LMR9, B5VEJ3, C7GMY8, D3UEZ1, G2W9P6, N1P9M0

Conserved Domains (1) summary

cd06855
Location:43 → 352

GT_GPT_euk; UDP-GlcNAc:dolichol-P GlcNAc-1-P transferase (GPT) catalyzes the transfer of GlcNAc-1-P from UDP-GlcNAc to dolichol-P to form GlcNAc-P-P-dolichol. The reaction is the first step in the assembly of dolichol-linked oligosaccharide intermediates and is ...