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SKI8 SKI complex subunit WD repeat protein SKI8 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852659, updated on 13-Apr-2024

Summary

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Gene symbol
SKI8
Gene description
SKI complex subunit WD repeat protein SKI8
Primary source
SGD:S000003181
Locus tag
YGL213C
See related
AllianceGenome:SGD:S000003181
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
REC103
Summary
Involved in nuclear-transcribed mRNA catabolic process; protein-DNA complex assembly; and reciprocal meiotic recombination. Located in nuclear chromosome. Part of Ski complex. Orthologous to human WDR61 (WD repeat domain 61). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (90054..91247, complement)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene tubulin-dependent ATPase KIP3 Neighboring gene Clg1p Neighboring gene Vam7p Neighboring gene Ncs6p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure. Madrona AY, et al. Protein Sci, 2004 Jun. PMID 15152089, Free PMC Article
  2. A yeast antiviral protein, SKI8, shares a repeated amino acid sequence pattern with beta-subunits of G proteins and several other proteins. Matsumoto Y, et al. Yeast, 1993 Jan. PMID 8442386
  3. Gene disruption indicates that the only essential function of the SKI8 chromosomal gene is to protect Saccharomyces cerevisiae from viral cytopathology. Sommer SS, et al. Virology, 1987 Mar. PMID 3029964
  4. The yeast Ski complex is a hetero-tetramer. Synowsky SA, et al. Protein Sci, 2008 Jan. PMID 18042677, Free PMC Article
  5. The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif. Dunckley T, et al. EMBO J, 1999 Oct 1. PMID 10508173, Free PMC Article

See all (80) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Ski2, Ski3, and Ski8 assemble in a tetramer with 1:1:2 stoichiometry; 370 kDa core complex shows that Ski3 forms an array of 33 tetratricopeptide repeat motifs organized in N-terminal and C-terminal arms. The C-terminal arm of Ski3 and the two Ski8 subunits position the helicase core of Ski2 centrally within the complex, enhancing RNA binding.
    Title: The yeast ski complex: crystal structure and RNA channeling to the exosome complex.
  2. Ski8p has seven WD repeats, demonstrating that many WD repeats have not yet been identified in sequences and also raises the possibility that the seven-bladed propeller may be the predominant fold for this family of proteins
    Title: The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure.
  3. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p
    Title: Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination.
  4. Ski3p serves as a scaffold protein with its C terminus interacting with Ski8p.
    Title: Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in defense response to virus IEA
Inferred from Electronic Annotation
more info
  
involved_in meiotic cell cycle IEA
Inferred from Electronic Annotation
more info
  
involved_in nuclear-transcribed mRNA catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nuclear-transcribed mRNA catabolic process, non-stop decay IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein-DNA complex assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein-containing complex assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in reciprocal meiotic recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in reciprocal meiotic recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in reciprocal meiotic recombination IPI
Inferred from Physical Interaction
more info
 PubMed 
Component Evidence Code Pubs
part_of Ski complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of Ski complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nuclear chromosome IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
SKI complex subunit WD repeat protein SKI8
NP_011302.1
  • Ski complex component and WD-repeat protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    90054..91247 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181078.1 → NP_011302.1  TPA: SKI complex subunit WD repeat protein SKI8 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011302.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VTU2, Q02793
    UniProtKB/TrEMBL
    A0A8H4FCS3, B3LHQ9, C8Z842, N1P427
    Conserved Domains (2) summary
    sd00039
    Location:19 → 60
    7WD40; WD40 repeat [structural motif]
    cl29593
    Location:13 → 351
    WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
4BUJ_C.1
4BUJ_D.1
4BUJ_G.1
4BUJ_H.1
Q02793.1

Gene LinkOut

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