U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

BUB3 Bub3p [ Saccharomyces cerevisiae S288C ]

Gene ID: 854191, updated on 13-Apr-2024

Summary

Go to the top of the page Help
Gene symbol
BUB3
Gene description
Bub3p
Primary source
SGD:S000005552
Locus tag
YOR026W
See related
AllianceGenome:SGD:S000005552
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
PAC9
Summary
Enables ubiquitin binding activity. Involved in mitotic DNA integrity checkpoint signaling and positive regulation of protein autoubiquitination. Located in kinetochore. Part of mitotic checkpoint complex. Orthologous to human BUB3 (BUB3 mitotic checkpoint protein). [provided by Alliance of Genome Resources, Apr 2022]
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
chromosome: XV
Exon count:
1
Sequence:
Chromosome: XV; NC_001147.6 (379781..380806)

Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene Ahc1p Neighboring gene NAD-dependent histone deacetylase HST3 Neighboring gene Hsp90 cochaperone STI1 Neighboring gene Cin5p

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Crystal structure of the spindle assembly checkpoint protein Bub3. Larsen NA, et al. J Mol Biol, 2004 Dec 3. PMID 15544799
  2. Recognizing chromosomes in trouble: association of the spindle checkpoint protein Bub3p with altered kinetochores and a unique defective centromere. Kerscher O, et al. Mol Cell Biol, 2003 Sep. PMID 12944469, Free PMC Article
  3. The anti-malarial artesunate is also active against cancer. Efferth T, et al. Int J Oncol, 2001 Apr. PMID 11251172
  4. Bub3 reads phosphorylated MELT repeats to promote spindle assembly checkpoint signaling. Primorac I, et al. Elife, 2013 Sep 24. PMID 24066227, Free PMC Article
  5. Structural analysis of Bub3 interactions in the mitotic spindle checkpoint. Larsen NA, et al. Proc Natl Acad Sci U S A, 2007 Jan 23. PMID 17227844, Free PMC Article

See all (131) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Yeast Fin1-PP1 dephosphorylates an Ipl1 substrate, Ndc80, to remove Bub1-Bub3 checkpoint proteins from the kinetochore during anaphase.
    Title: Yeast Fin1-PP1 dephosphorylates an Ipl1 substrate, Ndc80, to remove Bub1-Bub3 checkpoint proteins from the kinetochore during anaphase.
  2. Differential requirement for Bub1 and Bub3 in regulation of meiotic versus mitotic chromosome segregation.
    Title: Differential requirement for Bub1 and Bub3 in regulation of meiotic versus mitotic chromosome segregation.
  3. Bub3 has a previously unknown function at the kinetochore in activating APC/C-Cdc20 for normal mitotic progression.
    Title: Bub3 promotes Cdc20-dependent activation of the APC/C in S. cerevisiae.
  4. Results describe the crystal structure of Bub3p from Saccharomyces cerevisiae at 1.1 angstrom and a crystallographic R-factor of 15.3%, revealing seven authentic repeats.
    Title: The 1.1-angstrom structure of the spindle checkpoint protein Bub3p reveals functional regions.
  5. Data describe the crystal structure of the Saccharomyces cerevisiae protein Bub3p at 2.35 angstrom resolution.
    Title: Crystal structure of the spindle assembly checkpoint protein Bub3.
Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ubiquitin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in cell cycle IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic DNA integrity checkpoint signaling IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitotic spindle assembly checkpoint signaling IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of protein autoubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of bub1-bub3 complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in intracellular organelle IEA
Inferred from Electronic Annotation
more info
  
is_active_in kinetochore IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in kinetochore IDA
Inferred from Direct Assay
more info
 PubMed 
part_of mitotic checkpoint complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of mitotic checkpoint complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in nucleoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
Bub3p
NP_014669.1
  • Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001147.6 Reference assembly

    Range
    379781..380806
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183445.1 → NP_014669.1  TPA: Bub3p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014669.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W292, P26449
    UniProtKB/TrEMBL
    A0A8H4BWU3, G2WMQ7
    Conserved Domains (2) summary
    COG2319
    Location:2 → 317
    WD40; WD40 repeat [General function prediction only]
    sd00039
    Location:14 → 55
    7WD40; WD40 repeat [structural motif]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P26449.1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Other Literature Sources
Molecular Biology Databases
Research Materials
Tools
Miscellaneous