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pflB pyruvate formate-lyase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945514, updated on 4-Apr-2024

Summary

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Gene symbol
pflB
Gene description
pyruvate formate-lyase
Primary source
ASAP:ABE-0003071
Locus tag
b0903
See related
ECOCYC:EG10701
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0894; pfl
Summary
HT_Cmplx8_Cyt: [More information is available at EcoGene: EG10701].
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Genomic context

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Sequence:
NC_000913.3 (951272..953554, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative oxidoreductase YcaK Neighboring gene pyruvate formate-lyase activating enzyme Neighboring gene formate channel FocA Neighboring gene ribosomal protein S12 methylthiotransferase accessory factor YcaO

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Computer-aided design of the stability of pyruvate formate-lyase from Escherichia coli by site-directed mutagenesis. Yang DF, et al. Biosci Biotechnol Biochem, 2007 Mar. PMID 17341838
  2. Structure of Escherichia coli pyruvate formate-lyase with pyruvate. Lehtiö L, et al. Acta Crystallogr D Biol Crystallogr, 2002 Dec. PMID 12454503
  3. X-ray structure of pyruvate formate-lyase in complex with pyruvate and CoA. How the enzyme uses the Cys-418 thiyl radical for pyruvate cleavage. Becker A, et al. J Biol Chem, 2002 Oct 18. PMID 12163496
  4. Pyruvate formate lyase is structurally homologous to type I ribonucleotide reductase. Leppänen VM, et al. Structure, 1999 Jul 15. PMID 10425676
  5. Expression of pyruvate formate-lyase of Escherichia coli from the cloned structural gene. Pecher A, et al. Arch Microbiol, 1982 Oct. PMID 6758723

See all (42) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. FocA has a strong preference for formate as a substrate in vivo and not other acidic fermentation products and the tight coupling between FocA and PflB synthesis ensures adequate substrate delivery to the appropriate formate dehydrogenase.
    Title: Coordination of FocA and pyruvate formate-lyase synthesis in Escherichia coli demonstrates preferential translocation of formate over other mixed-acid fermentation products.
  2. The gene encoding malate dehydrogenase (MDH) was overexpressed in a pflB ldhA double mutant, NZN111, for succinic acid production.
    Title: Production of succinate by a pflB ldhA double mutant of Escherichia coli overexpressing malate dehydrogenase.
  3. The authors found that the MG1655 pflB deletion mutant is able to grow in mineral media with glucose as the sole carbon source under fermentative conditions.
    Title: ATP limitation in a pyruvate formate lyase mutant of Escherichia coli MG1655 increases glycolytic flux to D-lactate.
  4. Deletion of the E. coli plfB gene (together with deletions of other ferementation disruption genes) increased production of succinate
    Title: Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C.
  5. N-terminus verified by Edman degradation on mature peptide
    Title: Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis.
  6. The pyruvate formate-lyase pathway is the major route for acetyl-CoA and formate production under anaerobic conditions.
    Title: Expression of the pfl gene and resulting metabolite flux distribution in nuo and ackA-pta E. coli mutant strains.
  7. essential for anaerobic growth of E. coli on xylose due to energetic constraints
    Title: Pyruvate formate lyase and acetate kinase are essential for anaerobic growth of Escherichia coli on xylose.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables formate C-acetyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables formate C-acetyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables formate C-acetyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glucose metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 

General protein information

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Preferred Names
pyruvate formate-lyase
NP_415423.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    951272..953554 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415423.1 pyruvate formate-lyase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415423.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T6BI39
    Conserved Domains (1) summary
    cd01678
    Location:11 → 756
    PFL1; Pyruvate formate lyase 1
Nucleotide Protein
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