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tolA Tol-Pal system protein TolA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946625, updated on 11-Apr-2024

Summary

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Gene symbol
tolA
Gene description
Tol-Pal system protein TolA
Primary source
ASAP:ABE-0002515
Locus tag
b0739
See related
ECOCYC:EG11007
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
cim; ECK0728; excC; lky; tol-2
Summary
Cell envelope integrity is stabilized by the Tol-Pal system linking inner and outer membranes. [More information is available at EcoGene: EG11007]. TolA is one of three (along with TolQ and TolR) inner membrane protein members of the YbgC-YbgF-TolQ-R-A-B-Pal Cell Envelope Complex, also known as the Tol-Pal system. [More information is available at EcoCyc: EG11007].
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Genomic context

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Sequence:
NC_000913.3 (776342..777607)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene esterase/thioesterase Neighboring gene Tol-Pal system protein TolQ Neighboring gene Tol-Pal system protein TolR Neighboring gene Tol-Pal system periplasmic protein TolB Neighboring gene peptidoglycan-associated outer membrane lipoprotein Pal

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The role for TolA in enterohemorrhagic Escherichia coli pathogenesis and virulence gene transcription. Morgan JK, et al. Microb Pathog, 2014 Dec. PMID 25448467
  2. Variation of intragenic tandem repeat tract of tolA modulates Escherichia coli stress tolerance. Zhou K, et al. PLoS One, 2012. PMID 23094082, Free PMC Article
  3. Structural evidence that colicin A protein binds to a novel binding site of TolA protein in Escherichia coli periplasm. Li C, et al. J Biol Chem, 2012 Jun 1. PMID 22493500, Free PMC Article
  4. Characterisation of the interaction of colicin A with its co-receptor TolA. Hecht O, et al. FEBS Lett, 2010 Jun 3. PMID 20433837
  5. Energy-dependent immunity protein release during tol-dependent nuclease colicin translocation. Vankemmelbeke M, et al. J Biol Chem, 2009 Jul 10. PMID 19458090, Free PMC Article

See all (74) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Colicin N and M13 phage protein G3p compete with TolB for a binding site on TolA.
    Title: Antibacterial toxin colicin N and phage protein G3p compete with TolB for a binding site on TolA.
  2. Despite elevated virulence gene transcription, in tolA deleted strains virulence of enterohemorrhagic Escherichia coli in the Galleria mellonella wax worm model was substantially attenuated.
    Title: The role for TolA in enterohemorrhagic Escherichia coli pathogenesis and virulence gene transcription.
  3. Variation of intragenic tandem repeat tract of tolA modulates Escherichia coli stress tolerance.
    Title: Variation of intragenic tandem repeat tract of tolA modulates Escherichia coli stress tolerance.
  4. Using a combination of in vivo coimmunoprecipitations and in vitro pulldown experiments, we demonstrated that KT interacts with the TolA, TolB, and TolR proteins.
    Title: Interaction of the colicin K bactericidal toxin with components of its import machinery in the periplasm of Escherichia coli.
  5. The authors show that the stable YbgF trimer undergoes a marked change in oligomeric state on binding TolA, forming a one-to-one complex with the Tol protein.
    Title: TolA modulates the oligomeric status of YbgF in the bacterial periplasm.
  6. energy transduced from the cytoplasmic membrane to the outer membrane by TolA could be the driving force for immunity protein release and concomitant translocation of the nuclease domain
    Title: Energy-dependent immunity protein release during tol-dependent nuclease colicin translocation.
  7. The Y. enterocolitica TolA supported the physiological function of the Tol system in E. coli, and conferred sensitivity to the TolA-dependent colicins.
    Title: Differential complementation of DeltatolA Escherichia coli by a Yersinia enterocolitica TolA homologue.
  8. 3D structure of free TolAIII by heteronuclear NMR spectroscopy
    Title: Solution structure of the E.coli TolA C-terminal domain reveals conformational changes upon binding to the phage g3p N-terminal domain.
  9. in vivo and in vitro coprecipitation analyses demonstrated that colicin A and g3p N-terminal domains compete for binding to TolA.
    Title: Tol-dependent macromolecule import through the Escherichia coli cell envelope requires the presence of an exposed TolA binding motif.
  10. absence of TolA and Pal elicits a sustained extracytoplasmic stress response that in turn reduces O-antigen polymerization but does not affect the stability of the Wzy O-antigen polymerase
    Title: Defective O-antigen polymerization in tolA and pal mutants of Escherichia coli in response to extracytoplasmic stress.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables disordered domain specific binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein domain specific binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables toxin transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables virion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in bacteriocin transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within bacteriocin transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cell cycle IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell division IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cell division IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell division IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cell division NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within cellular response to bacteriocin IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within protein import IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of membrane invagination NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within symbiont entry into host cell IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within symbiont entry into host cell IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cell division site IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

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Preferred Names
Tol-Pal system protein TolA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    776342..777607
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415267.1 Tol-Pal system protein TolA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415267.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8I0CBZ0
    Conserved Domains (1) summary
    PRK09510
    Location:1 → 421
    tolA; cell envelope integrity inner membrane protein TolA; Provisional
Nucleotide Protein
Heading Accession and Version

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