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nrdB ribonucleoside-diphosphate reductase 1 subunit beta [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946732, updated on 4-Feb-2024

Summary

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Gene symbol
nrdB
Gene description
ribonucleoside-diphosphate reductase 1 subunit beta
Primary source
ASAP:ABE-0007386
Locus tag
b2235
See related
ECOCYC:EG10661
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2227; ftsB
Summary
Reducing equivalents provided by glutaredoxin or thioredoxin. [More information is available at EcoGene: EG10661]. The B2 protein of ribonucleoside-diphosphate reductase contains the tyrosyl radical-dinuclear iron center, which is thought to initiate catalysis by long-range electron transfer. [More information is available at EcoCyc: EG10661].
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Genomic context

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Sequence:
NC_000913.3 (2347384..2348514)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YpaB Neighboring gene ribonucleoside-diphosphate reductase 1 subunit alpha Neighboring gene protein YoaM Neighboring gene ferredoxin-like diferric-tyrosyl radical cofactor maintenance protein YfaE Neighboring gene putative lipopolysaccharide kinase InaA Neighboring gene pseudo

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Redox-linked structural changes in ribonucleotide reductase. Offenbacher AR, et al. J Am Chem Soc, 2009 Jun 10. PMID 19489635, Free PMC Article
  2. Cation mediation of radical transfer between Trp48 and Tyr356 during O2 activation by protein R2 of Escherichia coli ribonucleotide reductase: relevance to R1-R2 radical transfer in nucleotide reduction? Saleh L, et al. Biochemistry, 2006 Jul 25. PMID 16846225
  3. Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122. Sommerhalter M, et al. Acta Crystallogr D Biol Crystallogr, 2005 Dec. PMID 16301799
  4. Local and global effects of metal binding within the small subunit of ribonucleotide reductase. Pierce BS, et al. J Am Chem Soc, 2005 Mar 16. PMID 15755183
  5. Characterization of NO adducts of the diiron center in protein R2 of Escherichia coli ribonucleotide reductase and site-directed variants; implications for the O2 activation mechanism. Lu S, et al. J Biol Inorg Chem, 2004 Oct. PMID 15311337

See all (113) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Elevated Levels of the Escherichia coli nrdAB-Encoded Ribonucleotide Reductase Counteract the Toxicity Caused by an Increased Abundance of the beta Clamp.
    Title: Elevated Levels of the Escherichia coli nrdAB-Encoded Ribonucleotide Reductase Counteract the Toxicity Caused by an Increased Abundance of the β Clamp.
  2. Subunit Interaction Dynamics of Class Ia Ribonucleotide Reductases: In Search of a Robust Assay.
    Title: Subunit Interaction Dynamics of Class Ia Ribonucleotide Reductases: In Search of a Robust Assay.
  3. The present results identifying a large, new set of E. coli mutator strains with mutations of nrdAB ribonucleotide reductase(RNR)confirm the important control that RNR exerts on the cellular mutation rate and provide novel insights into the RNR feedback regulatory mechanisms.
    Title: Novel mutator mutants of E. coli nrdAB ribonucleotide reductase: insight into allosteric regulation and control of mutation rates.
  4. N-terminus verified by Edman degradation on mature peptide
    Title: Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase.
  5. analysis of key residues in the dimerization process of ribonucleotide reductase protein R1
    Title: Nucleotide-dependent formation of catalytically competent dimers from engineered monomeric ribonucleotide reductase protein R1.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables iron ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
contributes_to ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in 2'-deoxyribonucleotide biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within deoxyribonucleotide biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in deoxyribonucleotide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within nucleobase-containing small molecule interconversion IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ribonucleoside diphosphate metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of ribonucleoside-diphosphate reductase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of ribonucleoside-diphosphate reductase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
ribonucleoside-diphosphate reductase 1 subunit beta
NP_416738.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2347384..2348514
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416738.1 ribonucleoside-diphosphate reductase 1 subunit beta [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416738.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T5YJX1
    Conserved Domains (1) summary
    PRK09101
    Location:1 → 376
    nrdB; ribonucleotide-diphosphate reductase subunit beta; Reviewed
Nucleotide Protein
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