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hisS histidine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946989, updated on 2-May-2024

Summary

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Gene symbol
hisS
Gene description
histidine--tRNA ligase
Primary source
ASAP:ABE-0008278
Locus tag
b2514
See related
ECOCYC:EG10453
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2510
Summary
Histidyl-tRNA synthetase (HisRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10453].
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Genomic context

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Sequence:
NC_000913.3 (2639301..2640575, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene outer membrane protein assembly factor BamB Neighboring gene ancillary SecYEG translocon subunit Neighboring gene ncRNA Neighboring gene (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin)

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Orientation and distance dependent chiral discrimination in the first step of the aminoacylation reaction: integrated molecular orbital and semi-empirical method (ONIOM) based calculation. Banik SD, et al. Colloids Surf B Biointerfaces, 2009 Dec 1. PMID 19682871
  2. Asymmetric amino acid activation by class II histidyl-tRNA synthetase from Escherichia coli. Guth E, et al. J Biol Chem, 2009 Jul 31. PMID 19487703, Free PMC Article
  3. Kinetic discrimination of tRNA identity by the conserved motif 2 loop of a class II aminoacyl-tRNA synthetase. Guth EC, et al. Mol Cell, 2007 Feb 23. PMID 17317626, Free PMC Article
  4. G-1:C73 recognition by an arginine cluster in the active site of Escherichia coli histidyl-tRNA synthetase. Connolly SA, et al. Biochemistry, 2004 Feb 3. PMID 14744140
  5. Recognition of G-1:C73 atomic groups by Escherichia coli histidyl-tRNA synthetase. Rosen AE, et al. J Am Chem Soc, 2004 Jan 14. PMID 14709061

See all (36) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Primary structure of histidine-tRNA synthetase and characterization of hisS transcripts.
  2. Surprising conclusion from study of concerted, substrate assisted mechanism of aminoacylation by histidyl-tRNA synthetase is that the overall rate of aminoacylation is slower than individual rates of either amino acid activation or aminoacyl transfer.
    Title: A substrate-assisted concerted mechanism for aminoacylation by a class II aminoacyl-tRNA synthetase.
  3. By use of a rapid kinetics analysis employing mutants in tRNA(His) and its cognate aminoacyl-tRNA synthetase, the role of tRNA identity in aminoacylation was investigated.
    Title: Kinetic discrimination of tRNA identity by the conserved motif 2 loop of a class II aminoacyl-tRNA synthetase.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables histidine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables histidine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables histidine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in histidyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within histidyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in histidyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
histidine--tRNA ligase
NP_417009.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2639301..2640575 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417009.1 histidine--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417009.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2W6PA59
    Conserved Domains (1) summary
    PRK00037
    Location:4 → 424
    hisS; histidyl-tRNA synthetase; Reviewed
Nucleotide Protein
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Gene LinkOut

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Other Literature Sources
Molecular Biology Databases
Research Materials