gapA glyceraldehyde-3-phosphate dehydrogenase A [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: gapA
Gene description: glyceraldehyde-3-phosphate dehydrogenase A
Primary source: EcoGene:EG10367
Locus tag: b1779
See related: ASAP:ABE-0005920; ECOCYC:EG10367
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1777; gad; gap1
Summary: A gapA mutant is not in the Keio collection; gapA mutants were not expected to grow on the medium used to attempt to isolate them; gapA is not an essential gene (Baba, 2006). [More information is available at EcoGene: EG10367]. Escherichia coli is unusual in having two glyceraldehyde-3-phosphate dehydrogenases. [More information is available at EcoCyc: EG10367].

Genomic context

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Sequence:: NC_000913.3 (1862771..1863766)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

The authors report that GAPDH of these pathogens is ADP-ribosylated either in the cytoplasm or in the extracellular medium. GAPDH catalyzes its own modification, which involves Cys-149 at the active site.
Title: NAD+-dependent post-translational modification of Escherichia coli glyceraldehyde-3-phosphate dehydrogenase.
N-terminus verified by Edman degradation on mature peptide
Title: Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins.
Data indicate that exported glyceraldehyde-3-phosphate dehydrogenase may act as a virulence factor which could contribute to bacterial pathogenesis.
Title: Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli: interaction of the extracellular enzyme with human plasminogen and fibrinogen.
promoter gapA P1 is a very efficient promoter sharing common properties with both extended -10 and non-extended -10 promoters
Title: The strong efficiency of the Escherichia coli gapA P1 promoter depends on a complex combination of functional determinants.

Submit: New GeneRIF Correction

Pathways from BioSystems

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Biosynthesis of amino acids, organism-specific biosystem (from KEGG)
Biosynthesis of amino acids, organism-specific biosystemThis map presents a modular architecture of the biosynthesis pathways of twenty amino acids, which may be viewed as consisting of the core part and its extensions. The core part is the KEGG module fo...
Biosynthesis of amino acids, conserved biosystem (from KEGG)
Biosynthesis of amino acids, conserved biosystemThis map presents a modular architecture of the biosynthesis pathways of twenty amino acids, which may be viewed as consisting of the core part and its extensions. The core part is the KEGG module fo...
Biosynthesis of antibiotics, organism-specific biosystem (from KEGG)
Biosynthesis of antibiotics, organism-specific biosystem
Biosynthesis of antibiotics
Biosynthesis of secondary metabolites, organism-specific biosystem (from KEGG)
Biosynthesis of secondary metabolites, organism-specific biosystem
Biosynthesis of secondary metabolites
Carbon metabolism, organism-specific biosystem (from KEGG)
Carbon metabolism, organism-specific biosystemCarbon metabolism is the most basic aspect of life. This map presents an overall view of central carbon metabolism, where the number of carbons is shown for each compound denoted by a circle, excludi...
Carbon metabolism, conserved biosystem (from KEGG)
Carbon metabolism, conserved biosystemCarbon metabolism is the most basic aspect of life. This map presents an overall view of central carbon metabolism, where the number of carbons is shown for each compound denoted by a circle, excludi...
D-galactonate degradation, De Ley-Doudoroff pathway, D-galactonate => glycerate-3P, organism-specific biosystem (from KEGG)
D-galactonate degradation, De Ley-Doudoroff pathway, D-galactonate => glycerate-3P, organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Other carbohydrate metabolism
D-galactonate degradation, De Ley-Doudoroff pathway, D-galactonate => glycerate-3P, conserved biosystem (from KEGG)
D-galactonate degradation, De Ley-Doudoroff pathway, D-galactonate => glycerate-3P, conserved biosystemPathway module; Carbohydrate and lipid metabolism; Other carbohydrate metabolism
Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem (from KEGG)
Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem (from KEGG)
Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Glycolysis (Embden-Meyerhof pathway), glucose => pyruvate, organism-specific biosystem (from KEGG)
Glycolysis (Embden-Meyerhof pathway), glucose => pyruvate, organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Glycolysis (Embden-Meyerhof pathway), glucose => pyruvate, conserved biosystem (from KEGG)
Glycolysis (Embden-Meyerhof pathway), glucose => pyruvate, conserved biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Glycolysis / Gluconeogenesis, organism-specific biosystem (from KEGG)
Glycolysis / Gluconeogenesis, organism-specific biosystemGlycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabol...
Glycolysis / Gluconeogenesis, conserved biosystem (from KEGG)
Glycolysis / Gluconeogenesis, conserved biosystemGlycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabol...
Glycolysis, core module involving three-carbon compounds, organism-specific biosystem (from KEGG)
Glycolysis, core module involving three-carbon compounds, organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Glycolysis, core module involving three-carbon compounds, conserved biosystem (from KEGG)
Glycolysis, core module involving three-carbon compounds, conserved biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Metabolic pathways, organism-specific biosystem (from KEGG)
Metabolic pathways, organism-specific biosystem
Metabolic pathways
Microbial metabolism in diverse environments, organism-specific biosystem (from KEGG)
Microbial metabolism in diverse environments, organism-specific biosystem
Microbial metabolism in diverse environments
gluconeogenesis I, organism-specific biosystem (from BIOCYC)
gluconeogenesis I, organism-specific biosystemEscherichia coli uses gluconeogenesis to synthesize glucose 6-phosphate from non-carbohydrate carbon substrates when sufficient amounts of exogenous glucose or other sugars are unavailable for transp...
gluconeogenesis I, conserved biosystem (from BIOCYC)
gluconeogenesis I, conserved biosystemGluconeogenesis is the generation of glucose from non-sugar carbon substrates such as |FRAME: PYRUVATE|, |FRAME: L-LACTATE|, |FRAME: GLYCEROL|, and glucogenic amino acids (primarily |FRAME: L-ALPHA-A...
glycolysis I (from glucose 6-phosphate), organism-specific biosystem (from BIOCYC)
glycolysis I (from glucose 6-phosphate), organism-specific biosystemGeneral Background Glycolysis, which was first studied as a pathway for the utilization of glucose, is one of the components of central metabolism, the other two being the :PENTOSE-P-PWY and the :TC...
glycolysis I (from glucose 6-phosphate), conserved biosystem (from BIOCYC)
glycolysis I (from glucose 6-phosphate), conserved biosystemGeneral Background Glycolysis, which was first studied as a pathway for the utilization of glucose, is one of the major pathways of central metabolism, the other two being the |FRAME:PENTOSE-P-PWY| ...
glycolysis II (from fructose 6-phosphate), organism-specific biosystem (from BIOCYC)
glycolysis II (from fructose 6-phosphate), organism-specific biosystemGeneral Background Glycolysis, which was first studied as a pathway for the utilization of glucose, is one of the components of central metabolism, the other two being the :PENTOSE-P-PWY and the :TC...
glycolysis II (from fructose 6-phosphate), conserved biosystem (from BIOCYC)
glycolysis II (from fructose 6-phosphate), conserved biosystemGeneral Background Glycolysis, which was first studied as a pathway for the utilization of glucose, is one of the major pathways of central metabolism, the other two being the |FRAME:PENTOSE-P-PWY| ...
superpathway of N-acetylneuraminate degradation, conserved biosystem (from BIOCYC)
superpathway of N-acetylneuraminate degradation, conserved biosystemSeveral viridans streptococci, such as |FRAME: TAX-1303|, are able to enter the bloodstream through dental caries and cause several serious infections, including endocarditis, brain abscesses and, in...
superpathway of glycolysis and Entner-Doudoroff, organism-specific biosystem (from BIOCYC)
superpathway of glycolysis and Entner-Doudoroff, organism-specific biosystem
superpathway of glycolysis and Entner-Doudoroff
superpathway of glycolysis and Entner-Doudoroff, conserved biosystem (from BIOCYC)
superpathway of glycolysis and Entner-Doudoroff, conserved biosystem
superpathway of glycolysis and Entner-Doudoroff
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass, organism-specific biosystem (from BIOCYC)
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass, organism-specific biosystem
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass, conserved biosystem (from BIOCYC)
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass, conserved biosystem
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
superpathway of hexitol degradation (bacteria), organism-specific biosystem (from BIOCYC)
superpathway of hexitol degradation (bacteria), organism-specific biosystemOf the ten existing hexitols (: CPD-15703, : CPD-12811, : CPD-15702, : SORBITOL, : CPD-12810, : MANNITOL, : CPD-15701, : CPD-357, : CPD-369 and : GALACTITOL) only three occur naturally - : MANNITOL, ...
superpathway of hexitol degradation (bacteria), conserved biosystem (from BIOCYC)
superpathway of hexitol degradation (bacteria), conserved biosystemOf the ten existing hexitols (|FRAME: CPD-15703|, |FRAME: CPD-12811|, |FRAME: CPD-15702|, |FRAME: SORBITOL|, |FRAME: CPD-12810|, |FRAME: MANNITOL|, |FRAME: CPD-15701|, |FRAME: CPD-357|, |FRAME: CPD-3...

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by GO

Function	Evidence Code	Pubs
NAD binding	IDA Inferred from Direct Assay more info	PubMed
NAD binding	IPI Inferred from Physical Interaction more info	PubMed
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity	IDA Inferred from Direct Assay more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
glycolytic process	IBA Inferred from Biological aspect of Ancestor more info	PubMed
glycolytic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
cytosol	IBA Inferred from Biological aspect of Ancestor more info	PubMed
cytosol	IDA Inferred from Direct Assay more info	PubMed
membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: glyceraldehyde-3-phosphate dehydrogenase A

NP_416293.1

EC 1.2.1.12

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1862771..1863766

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416293.1 glyceraldehyde-3-phosphate dehydrogenase A [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416293.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P0A9B2

Conserved Domains (1) summary

PRK15425
Location:1 → 331

gapA; glyceraldehyde-3-phosphate dehydrogenase A; Provisional