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glmS L-glutamine--D-fructose-6-phosphate aminotransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948241, updated on 4-Apr-2024

Summary

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Gene symbol
glmS
Gene description
L-glutamine--D-fructose-6-phosphate aminotransferase
Primary source
ASAP:ABE-0012198
Locus tag
b3729
See related
ECOCYC:EG10382
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3722
Summary
GlmS synthesis s negatively feedback regulated by glucosamine-6-phosphate, which probably causes YhbJ to cleave and inactivate the GlmZ sRNA; GlmZ upregulates glmS mRNA post-translationally (Kalamorz, 2007). [More information is available at EcoGene: EG10382]. L-glutamine:D-fructose-6-phosphate aminotransferase, or GFAT, catalyzes the first step in hexosamine biosynthesis. [More information is available at EcoCyc: EG10382].
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Genomic context

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Sequence:
NC_000913.3 (3911839..3913668, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene phosphate ABC transporter membrane subunit PstA Neighboring gene phosphate ABC transporter membrane subunit PstC Neighboring gene phosphate ABC transporter periplasmic binding protein Neighboring gene fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase Neighboring gene ATP synthase F1 complex subunit epsilon

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Application of GFAT as a novel selection marker to mediate gene expression. Wu G, et al. PLoS One, 2011 Feb 14. PMID 21340036, Free PMC Article
  2. Ordering of C-terminal loop and glutaminase domains of glucosamine-6-phosphate synthase promotes sugar ring opening and formation of the ammonia channel. Mouilleron S, et al. J Mol Biol, 2008 Apr 4. PMID 18295797
  3. Ammonia channeling in bacterial glucosamine-6-phosphate synthase (Glms): molecular dynamics simulations and kinetic studies of protein mutants. Floquet N, et al. FEBS Lett, 2007 Jun 26. PMID 17559838
  4. Directed evolution and characterization of Escherichia coli glucosamine synthase. Deng MD, et al. Biochimie, 2006 May. PMID 16871653
  5. Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase. Mouilleron S, et al. J Biol Chem, 2006 Feb 17. PMID 16339762

See all (56) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. accumulation of GlmZ* prevents complete GlmZ turnover. This mechanism may serve to adjust a robust glmS basal expression level that is buffered against fluctuations in RapZ availability.
    Title: Feedback regulation of small RNA processing by the cleavage product.
  2. An effective technique for the construction of GM (genetically modified) bacteria without an antibiotic resistant marker, and the construction of GM yeasts to be applied to complex media.
    Title: Application of GFAT as a novel selection marker to mediate gene expression.
  3. Analysis of how GlmZ, GlmY and PAP I act together in the regulation of glmS expression.
    Title: The small RNA GlmY acts upstream of the sRNA GlmZ in the activation of glmS expression and is subject to regulation by polyadenylation in Escherichia coli.
  4. The biological function of GlmZ is to positively control the glmS mRNA in response to GlcN-6-P concentrations and that YhbJ negatively regulates GlmZ. As in yhbJ mutants GlcN-6-P has no effect, YhbJ is essential for sensing this metabolite.
    Title: Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli.
  5. N-terminus verified by Edman degradation on mature peptide
    Title: Glucosamine synthetase from Escherichia coli: purification, properties, and glutamine-utilizing site location.
  6. Ammonia channeling in bacterial glucosamine-6-phosphate synthase (Glms).
    Title: Ammonia channeling in bacterial glucosamine-6-phosphate synthase (Glms): molecular dynamics simulations and kinetic studies of protein mutants.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables carbohydrate derivative binding IEA
Inferred from Electronic Annotation
more info
  
enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transaminase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within UDP-N-acetylglucosamine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in UDP-N-acetylglucosamine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in carbohydrate derivative biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in carbohydrate derivative metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in fructose 6-phosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glutamine metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in protein N-linked glycosylation IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
L-glutamine--D-fructose-6-phosphate aminotransferase
NP_418185.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3911839..3913668 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418185.1 L-glutamine--D-fructose-6-phosphate aminotransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418185.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    Q0P6L2
    Conserved Domains (1) summary
    PRK00331
    Location:1 → 609
    PRK00331; isomerizing glutamine--fructose-6-phosphate transaminase
Nucleotide Protein
Heading Accession and Version

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