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mutL DNA mismatch repair protein MutL [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948691, updated on 4-Apr-2024

Summary

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Gene symbol
mutL
Gene description
DNA mismatch repair protein MutL
Primary source
ASAP:ABE-0013655
Locus tag
b4170
See related
ECOCYC:EG11281
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4166
Summary
MutL DNA-binding is required for methyl-directed mismatch repair (Robertson, 2006). [More information is available at EcoGene: EG11281]. MutL is one of the components of the MutHLS complex. [More information is available at EcoCyc: EG11281].
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Genomic context

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Sequence:
NC_000913.3 (4397412..4399259)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene NAD(P)HX epimerase/NAD(P)HX dehydratase Neighboring gene N(6)-L-threonylcarbamoyladenine synthase, TsaE subunit Neighboring gene N-acetylmuramoyl-L-alanine amidase B Neighboring gene tRNA dimethylallyltransferase Neighboring gene RNA-binding protein Hfq Neighboring gene ribosome rescue factor HflX

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. MutL binds to 3' resected DNA ends and blocks DNA polymerase access. Borsellini A, et al. Nucleic Acids Res, 2022 Jun 24. PMID 35670670, Free PMC Article
  2. Probing the DNA-binding center of the MutL protein from the Escherichia coli mismatch repair system via crosslinking and Förster resonance energy transfer. Monakhova M, et al. Biochimie, 2020 Apr-May. PMID 32061805
  3. Insights from a decade of biophysical studies on MutL: Roles in strand discrimination and mismatch removal. Guarné A, et al. Prog Biophys Mol Biol, 2015 Mar. PMID 25701376
  4. Characterization of the defects in the ATP lid of E. coli MutL that cause transient hypermutability. Pillon MC, et al. DNA Repair (Amst), 2013 Oct. PMID 23916559
  5. Evidence for ATP-dependent structural rearrangement of nuclease catalytic site in DNA mismatch repair endonuclease MutL. Yamamoto T, et al. J Biol Chem, 2011 Dec 9. PMID 21953455, Free PMC Article

See all (116) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MutL binds to 3' resected DNA ends and blocks DNA polymerase access.
    Title: MutL binds to 3' resected DNA ends and blocks DNA polymerase access.
  2. The selection process of licensing a DNA mismatch for repair.
    Title: The selection process of licensing a DNA mismatch for repair.
  3. Responses of DNA Mismatch Repair Proteins to a Stable G-Quadruplex Embedded into a DNA Duplex Structure.
    Title: Responses of DNA Mismatch Repair Proteins to a Stable G-Quadruplex Embedded into a DNA Duplex Structure.
  4. Probing the DNA-binding center of the MutL protein from the Escherichia coli mismatch repair system via crosslinking and Forster resonance energy transfer.
    Title: Probing the DNA-binding center of the MutL protein from the Escherichia coli mismatch repair system via crosslinking and Förster resonance energy transfer.
  5. We further show that MutL is unable to activate the helicase activity of a chimeric UvrD containing the 2B subdomain of the structurally similar Rep helicase. Hence, MutL activation of the monomeric UvrD helicase is regulated specifically by its 2B subdomain.
    Title: UvrD helicase activation by MutL involves rotation of its 2B subdomain.
  6. Observations are consistent with an exonuclease-independent MMR strand excision mechanism that relies on EcMutL-EcUvrD helicase-driven displacement of ssDNA segments between adjacent EcMutH-GATC incisions.
    Title: MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair.
  7. In Bacillus subtilis, the interaction stimulates the endonuclease activity of MutL and it is critical for DNA mismatch repair. Conversely, disrupting the interaction between Escherichia coli MutL and the b-clamp only causes a mild mutator phenotype. Here, we determined the structures of the regulatory domains of E. coli and B. subtilis MutL bound to their respective b-clamps
    Title: Binding of the regulatory domain of MutL to the sliding β-clamp is species specific.
  8. The assembly of an EcMutS-EcMutL-EcMutH search complex illustrates how sequential stable sliding clamps can modulate one-dimensional diffusion mechanics along the DNA to direct mismatch repair
    Title: Cascading MutS and MutL sliding clamps control DNA diffusion to activate mismatch repair.
  9. The structure captures MutS in the sliding clamp conformation, where tilting of the MutS subunits across each other pushes DNA into a new channel, and reorientation of the connector domain creates an interface for MutL with both MutS subunits.
    Title: MutS/MutL crystal structure reveals that the MutS sliding clamp loads MutL onto DNA.
  10. In this review, we describe biochemical, biophysical and structural analyses that have clarified how MutL aids at discriminating the newly synthesized strand from its template and marking it for removal. [review]
    Title: Insights from a decade of biophysical studies on MutL: Roles in strand discrimination and mismatch removal.
Submit: New GeneRIF Correction See all GeneRIFs (32)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATP hydrolysis activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables ATP-dependent DNA damage sensor activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables mismatched DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in mismatch repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mismatch repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mismatch repair IEA
Inferred from Electronic Annotation
more info
  
involved_in mismatch repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in nucleotide-excision repair, DNA duplex unwinding IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within regulation of DNA recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA recombination NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of mismatch repair complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mismatch repair complex IC
Inferred by Curator
more info
 PubMed 
part_of mismatch repair complex IEA
Inferred from Electronic Annotation
more info
  
part_of mismatch repair complex NAS
Non-traceable Author Statement
more info
 PubMed 
part_of single-stranded DNA-dependent ATP-dependent DNA helicase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
DNA mismatch repair protein MutL

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4397412..4399259
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418591.1 DNA mismatch repair protein MutL [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418591.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7X036
    Conserved Domains (1) summary
    PRK00095
    Location:1 → 613
    mutL; DNA mismatch repair endonuclease MutL
Nucleotide Protein
Heading Accession and Version

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Other Literature Sources
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Research Materials